ID   NXT1_MOUSE              Reviewed;         140 AA.
AC   Q9QZV9; Q3THE3; Q9D1P7;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 2.
DT   03-AUG-2022, entry version 144.
DE   RecName: Full=NTF2-related export protein 1;
GN   Name=Nxt1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH NXF2.
RX   PubMed=10567585; DOI=10.1128/mcb.19.12.8616;
RA   Black B.E., Levesque L., Holaska J.M., Wood T.C., Paschal B.M.;
RT   "Identification of an NTF2-related factor that binds Ran-GTP and regulates
RT   nuclear protein export.";
RL   Mol. Cell. Biol. 19:8616-8624(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=BALB/cJ, and C57BL/6J; TISSUE=Embryo;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Czech II; TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Stimulator of protein export for NES-containing proteins.
CC       Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA.
CC       The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in
CC       conjunction with ALYREF/THOC4 and THOC5 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with NXF1. Forms a complex with RANGAP1,
CC       RANBP2/NUP358 and NXF1. Interacts (via NTF2 domain) with NXF1.
CC       Stabilizes the NTF2 domain of NXF1 by heterodimerization. The formation
CC       of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear
CC       mRNA export. Preferentially binds Ran-GTP. Associates with NXF2, NXF3
CC       and NXF5. Does not bind nucleoporins (NPC) directly, its association to
CC       NPC is mediated by NXF1. {ECO:0000250|UniProtKB:Q9UKK6}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Nucleus speckle {ECO:0000250}.
CC       Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm.
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DR   EMBL; AF156958; AAD54943.2; -; mRNA.
DR   EMBL; AK003254; BAB22670.1; -; mRNA.
DR   EMBL; AK168314; BAE40254.1; -; mRNA.
DR   EMBL; BC023672; AAH23672.1; -; mRNA.
DR   CCDS; CCDS16840.1; -.
DR   RefSeq; NP_001103629.1; NM_001110159.1.
DR   RefSeq; NP_062735.4; NM_019761.6.
DR   AlphaFoldDB; Q9QZV9; -.
DR   SMR; Q9QZV9; -.
DR   BioGRID; 208013; 7.
DR   IntAct; Q9QZV9; 2.
DR   MINT; Q9QZV9; -.
DR   STRING; 10090.ENSMUSP00000105587; -.
DR   PhosphoSitePlus; Q9QZV9; -.
DR   EPD; Q9QZV9; -.
DR   MaxQB; Q9QZV9; -.
DR   PaxDb; Q9QZV9; -.
DR   PeptideAtlas; Q9QZV9; -.
DR   PRIDE; Q9QZV9; -.
DR   ProteomicsDB; 287867; -.
DR   Antibodypedia; 24862; 109 antibodies from 21 providers.
DR   DNASU; 56488; -.
DR   Ensembl; ENSMUST00000047177; ENSMUSP00000038329; ENSMUSG00000036992.
DR   Ensembl; ENSMUST00000109961; ENSMUSP00000105587; ENSMUSG00000036992.
DR   GeneID; 56488; -.
DR   KEGG; mmu:56488; -.
DR   UCSC; uc008mtf.2; mouse.
DR   CTD; 29107; -.
DR   MGI; MGI:1929619; Nxt1.
DR   VEuPathDB; HostDB:ENSMUSG00000036992; -.
DR   eggNOG; KOG4353; Eukaryota.
DR   GeneTree; ENSGT00940000162041; -.
DR   HOGENOM; CLU_122448_1_1_1; -.
DR   InParanoid; Q9QZV9; -.
DR   OMA; THAFTQT; -.
DR   OrthoDB; 1450028at2759; -.
DR   PhylomeDB; Q9QZV9; -.
DR   TreeFam; TF318944; -.
DR   Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR   BioGRID-ORCS; 56488; 23 hits in 72 CRISPR screens.
DR   PRO; PR:Q9QZV9; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q9QZV9; protein.
DR   Bgee; ENSMUSG00000036992; Expressed in spermatocyte and 265 other tissues.
DR   Genevisible; Q9QZV9; MM.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005643; C:nuclear pore; IDA:MGI.
DR   GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR   GO; GO:0042272; C:nuclear RNA export factor complex; ISO:MGI.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; ISO:MGI.
DR   GO; GO:0031267; F:small GTPase binding; IDA:MGI.
DR   GO; GO:0006406; P:mRNA export from nucleus; ISO:MGI.
DR   GO; GO:0006913; P:nucleocytoplasmic transport; IBA:GO_Central.
DR   GO; GO:0006611; P:protein export from nucleus; IDA:MGI.
DR   GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR   GO; GO:0006405; P:RNA export from nucleus; IDA:MGI.
DR   CDD; cd00780; NTF2; 1.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR045875; NTF2/Mtr2.
DR   InterPro; IPR002075; NTF2_dom.
DR   InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR   PANTHER; PTHR12612; PTHR12612; 1.
DR   Pfam; PF02136; NTF2; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
DR   PROSITE; PS50177; NTF2_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cytoplasm; mRNA transport; Nucleus; Protein transport;
KW   Reference proteome; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UKK6"
FT   CHAIN           2..140
FT                   /note="NTF2-related export protein 1"
FT                   /id="PRO_0000194794"
FT   DOMAIN          16..135
FT                   /note="NTF2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00137"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UKK6"
FT   CONFLICT        100
FT                   /note="K -> N (in Ref. 2; BAB22670)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   140 AA;  15847 MW;  358EAFDC19FE4594 CRC64;
     MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN AVSGQESLSE
     FFEMLPSSEF QISVVDCQPV HDDATPSQTT VLVVICGTVK FEGNKQRDFN QNFILTAQAS
     PSNTVWKIAS DCFRFQDWAS