NYAP2_HUMAN
ID NYAP2_HUMAN Reviewed; 653 AA.
AC Q9P242; A2RRN4; Q96NL2;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 3.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2;
GN Name=NYAP2; Synonyms=KIAA1486;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10819331; DOI=10.1093/dnares/7.2.143;
RA Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XVII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:143-150(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18220336; DOI=10.1021/pr0705441;
RA Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III;
RT "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient
RT phosphoproteomic analysis.";
RL J. Proteome Res. 7:1346-1351(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-434, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Activates PI3K and concomitantly recruits the WAVE1 complex
CC to the close vicinity of PI3K and regulates neuronal morphogenesis.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ACOT9, ARHGAP26 and PIK3R2. Interacts with
CC components of the WAVE1 complex, CYFIP1 and NCKAP1; this interaction
CC mediates PI3K-WAVE1 association and actin cytoskeleton remodeling (By
CC similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q9P242; P52594: AGFG1; NbExp=3; IntAct=EBI-10269689, EBI-996560;
CC Q9P242; Q8NDC4: MORN4; NbExp=3; IntAct=EBI-10269689, EBI-10269566;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9P242-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9P242-2; Sequence=VSP_032432, VSP_032433;
CC Name=3;
CC IsoId=Q9P242-3; Sequence=VSP_032431, VSP_032434, VSP_032435;
CC -!- PTM: Phosphorylated on tyrosine residues by FYN upon stimulation with
CC CNTN5. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NYAP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA96010.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB040919; BAA96010.1; ALT_INIT; mRNA.
DR EMBL; AK055226; BAB70880.1; -; mRNA.
DR EMBL; AC016717; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC019231; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC067961; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC106890; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC131727; AAI31728.1; -; mRNA.
DR CCDS; CCDS46529.1; -. [Q9P242-1]
DR RefSeq; NP_065915.1; NM_020864.1. [Q9P242-1]
DR RefSeq; XP_005246765.1; XM_005246708.3. [Q9P242-1]
DR AlphaFoldDB; Q9P242; -.
DR BioGRID; 121670; 15.
DR IntAct; Q9P242; 5.
DR STRING; 9606.ENSP00000272907; -.
DR GlyGen; Q9P242; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9P242; -.
DR PhosphoSitePlus; Q9P242; -.
DR BioMuta; NYAP2; -.
DR DMDM; 296439320; -.
DR jPOST; Q9P242; -.
DR MassIVE; Q9P242; -.
DR MaxQB; Q9P242; -.
DR PaxDb; Q9P242; -.
DR PeptideAtlas; Q9P242; -.
DR PRIDE; Q9P242; -.
DR ProteomicsDB; 83723; -. [Q9P242-1]
DR ProteomicsDB; 83724; -. [Q9P242-2]
DR ProteomicsDB; 83725; -. [Q9P242-3]
DR Antibodypedia; 49421; 42 antibodies from 13 providers.
DR DNASU; 57624; -.
DR Ensembl; ENST00000272907.7; ENSP00000272907.6; ENSG00000144460.12. [Q9P242-1]
DR Ensembl; ENST00000636099.1; ENSP00000490942.1; ENSG00000144460.12. [Q9P242-1]
DR GeneID; 57624; -.
DR KEGG; hsa:57624; -.
DR MANE-Select; ENST00000272907.8; ENSP00000272907.7; NM_001371273.1; NP_001358202.1.
DR UCSC; uc002voe.3; human. [Q9P242-1]
DR CTD; 57624; -.
DR DisGeNET; 57624; -.
DR GeneCards; NYAP2; -.
DR HGNC; HGNC:29291; NYAP2.
DR HPA; ENSG00000144460; Tissue enhanced (brain).
DR MIM; 615478; gene.
DR neXtProt; NX_Q9P242; -.
DR OpenTargets; ENSG00000144460; -.
DR PharmGKB; PA165696738; -.
DR VEuPathDB; HostDB:ENSG00000144460; -.
DR eggNOG; ENOG502QTY4; Eukaryota.
DR GeneTree; ENSGT00890000139453; -.
DR HOGENOM; CLU_012558_1_0_1; -.
DR InParanoid; Q9P242; -.
DR OMA; PWLCGDA; -.
DR OrthoDB; 295010at2759; -.
DR PhylomeDB; Q9P242; -.
DR PathwayCommons; Q9P242; -.
DR SignaLink; Q9P242; -.
DR BioGRID-ORCS; 57624; 13 hits in 1064 CRISPR screens.
DR ChiTaRS; NYAP2; human.
DR GenomeRNAi; 57624; -.
DR Pharos; Q9P242; Tdark.
DR PRO; PR:Q9P242; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9P242; protein.
DR Bgee; ENSG00000144460; Expressed in cortical plate and 55 other tissues.
DR Genevisible; Q9P242; HS.
DR GO; GO:0048812; P:neuron projection morphogenesis; ISS:UniProtKB.
DR GO; GO:0014065; P:phosphatidylinositol 3-kinase signaling; ISS:UniProtKB.
DR InterPro; IPR026722; NYAP1/NYAP2.
DR InterPro; IPR026723; NYAP2.
DR InterPro; IPR029353; NYAP_C.
DR InterPro; IPR039482; NYAP_N.
DR PANTHER; PTHR22633; PTHR22633; 1.
DR PANTHER; PTHR22633:SF1; PTHR22633:SF1; 1.
DR Pfam; PF15452; NYAP_C; 1.
DR Pfam; PF15439; NYAP_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Methylation; Phosphoprotein; Reference proteome.
FT CHAIN 1..653
FT /note="Neuronal tyrosine-phosphorylated phosphoinositide-3-
FT kinase adapter 2"
FT /id="PRO_0000325830"
FT REGION 99..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..161
FT /note="Involved in CYFIP1- and NCKAP1-binding"
FT /evidence="ECO:0000250"
FT REGION 373..653
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 171..192
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..445
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..478
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 499..527
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 529..572
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 590..653
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 81
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q8BM65"
FT MOD_RES 120
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BM65"
FT MOD_RES 206
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BM65"
FT MOD_RES 268
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q8BM65"
FT MOD_RES 434
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT VAR_SEQ 1..486
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_032431"
FT VAR_SEQ 76..145
FT /note="GGEVGRGHEGSYVGKHFRMGFMTMPAPQDRLPHPCSSGFSVRSQSLHSVGGT
FT DDDSSCGSRRQPPPKPKR -> NRGTESPKPQHGAITKVGQQGKRPPWGVFLQRACQSS
FT GMGWNTRATCGHQSTRKMLCEPHLVSGKPQFRA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032432"
FT VAR_SEQ 146..653
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032433"
FT VAR_SEQ 645
FT /note="V -> I (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_032434"
FT VAR_SEQ 646..653
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_032435"
FT VARIANT 205
FT /note="T -> A (in dbSNP:rs7422680)"
FT /id="VAR_039933"
FT VARIANT 586
FT /note="T -> P (in dbSNP:rs3748993)"
FT /id="VAR_039934"
SQ SEQUENCE 653 AA; 70548 MW; 78451871EC7822C6 CRC64;
MISSKMMSSN PEEDPLDTFL QYIEDMGMKA YDGLVIQNAS DIARENDRLR NETNLAYLKE
KNEKRRRQEE AIKRIGGEVG RGHEGSYVGK HFRMGFMTMP APQDRLPHPC SSGFSVRSQS
LHSVGGTDDD SSCGSRRQPP PKPKRDPSTK LSTSSETVSS TAASKSGKTP ERTEASAKPR
PHSDEYSKKI PPPKPKRNPN TQLSTSFDET YIKKHGPRRT SLPRDSSLSQ MGSPAGDPEE
EEPVYIEMVG NILRDFRKED DDQSEAVYEE MKYPIFDDLG QDAKCDFDHH SCSSQCATPT
VPDLDFAKAS VPCPPKGLLC DIPPPFPNLL SHRPPLLVFP PAPVHCSPNS DESPLTPLEV
TKLPVLENVS YMKQPAGASP STLPSHVPGH AKLEKEQAAA LGPASATPAL SSSPPPPSTL
YRTQSPHGYP KSHSTSPSPV SMGRSLTPLS LKRPPPYDAV HSGSLSRSSP SVPHSTPRPV
SQDGAKMVNA AVNTYGAAPG GSRSRTPTSP LEELTSLFSS GRSLLRKSSS GRRSKEPAEK
STEELKVRSH STEPLPKLDN KERGHHGASS SREPVKAQEW DGTPGTPVVT SRLGRCSVSP
TLLAGNHSSE PKVSCKLGRS ASTSGVPPPS VTPLRQSSDL QQSQVPSSLA NRD
