ARPC5_YEAST
ID ARPC5_YEAST Reviewed; 154 AA.
AC P40518; D6VVM2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Actin-related protein 2/3 complex subunit 5;
DE AltName: Full=Arp2/3 complex 16 kDa subunit;
DE Short=p16-ARC;
GN Name=ARC15; OrderedLocusNames=YIL062C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION IN THE ARP2/3 COMPLEX.
RX PubMed=9210376; DOI=10.1016/s0960-9822(06)00223-5;
RA Winter D., Podtelejnikov A.V., Mann M., Li R.;
RT "The complex containing actin-related proteins Arp2 and Arp3 is required
RT for the motility and integrity of yeast actin patches.";
RL Curr. Biol. 7:519-529(1997).
RN [5]
RP ERRATUM OF PUBMED:9210376.
RA Winter D., Podtelejnikov A.V., Mann M., Li R.;
RL Curr. Biol. 7:R593-R593(1997).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=11248049; DOI=10.1073/pnas.051494698;
RA Boldogh I.R., Yang H.C., Nowakowski W.D., Karmon S.L., Hays L.G.,
RA Yates J.R. III, Pon L.A.;
RT "Arp2/3 complex and actin dynamics are required for actin-based
RT mitochondrial motility in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:3162-3167(2001).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-142, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Functions as component of the Arp2/3 complex which is
CC involved in regulation of actin polymerization and together with an
CC activating nucleation-promoting factor (NPF) mediates the formation of
CC branched actin networks. {ECO:0000250}.
CC -!- SUBUNIT: Component of the Arp2/3 complex composed of ARP2, ARP3,
CC ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and
CC ARC16/p16-ARC. {ECO:0000269|PubMed:9210376}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch
CC {ECO:0000269|PubMed:11248049}.
CC -!- MISCELLANEOUS: Present with 3060 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the ARPC5 family. {ECO:0000305}.
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DR EMBL; Z38060; CAA86161.1; -; Genomic_DNA.
DR EMBL; AY557850; AAS56176.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08488.1; -; Genomic_DNA.
DR PIR; S48417; S48417.
DR RefSeq; NP_012202.1; NM_001179412.1.
DR AlphaFoldDB; P40518; -.
DR SMR; P40518; -.
DR BioGRID; 34930; 424.
DR ComplexPortal; CPX-607; Arp2/3 complex.
DR DIP; DIP-2101N; -.
DR IntAct; P40518; 16.
DR MINT; P40518; -.
DR STRING; 4932.YIL062C; -.
DR iPTMnet; P40518; -.
DR MaxQB; P40518; -.
DR PaxDb; P40518; -.
DR PRIDE; P40518; -.
DR TopDownProteomics; P40518; -.
DR EnsemblFungi; YIL062C_mRNA; YIL062C; YIL062C.
DR GeneID; 854748; -.
DR KEGG; sce:YIL062C; -.
DR SGD; S000001324; ARC15.
DR VEuPathDB; FungiDB:YIL062C; -.
DR eggNOG; KOG3380; Eukaryota.
DR GeneTree; ENSGT00940000169405; -.
DR HOGENOM; CLU_101888_2_0_1; -.
DR InParanoid; P40518; -.
DR OMA; SQIQGRN; -.
DR BioCyc; YEAST:G3O-31330-MON; -.
DR Reactome; R-SCE-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR Reactome; R-SCE-5663213; RHO GTPases Activate WASPs and WAVEs.
DR Reactome; R-SCE-6798695; Neutrophil degranulation.
DR PRO; PR:P40518; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40518; protein.
DR GO; GO:0030479; C:actin cortical patch; IEA:UniProtKB-SubCell.
DR GO; GO:0015629; C:actin cytoskeleton; IC:ComplexPortal.
DR GO; GO:0005885; C:Arp2/3 protein complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031315; C:extrinsic component of mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:SGD.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IMP:SGD.
DR GO; GO:0044396; P:actin cortical patch organization; IMP:SGD.
DR GO; GO:0045010; P:actin nucleation; IC:ComplexPortal.
DR GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IBA:GO_Central.
DR GO; GO:0000001; P:mitochondrion inheritance; IMP:SGD.
DR GO; GO:0030833; P:regulation of actin filament polymerization; IEA:InterPro.
DR Gene3D; 1.25.40.190; -; 1.
DR InterPro; IPR006789; ARPC5.
DR InterPro; IPR036743; ARPC5_sf.
DR PANTHER; PTHR12644; PTHR12644; 1.
DR Pfam; PF04699; P16-Arc; 1.
DR PIRSF; PIRSF039096; p16-ARC; 1.
DR SUPFAM; SSF69103; SSF69103; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..154
FT /note="Actin-related protein 2/3 complex subunit 5"
FT /id="PRO_0000124059"
FT MOD_RES 142
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 154 AA; 17134 MW; 10E4DAF0F0801B02 CRC64;
MEADWRRIDI DAFDPESGRL TAADLVPPYE TTVTLQELQP RMNQLRSLAT SGDSLGAVQL
LTTDPPYSAD APTKEQYFKS VLEALTQVRQ ADIGNVIKNL SDSQRDVLVK YLYKGMSVPQ
GQKQGGVLLA WLERITQVSG VTPIVHYISD RRTV
