ID   ARPIN_BOVIN             Reviewed;         226 AA.
AC   Q17QU3;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Arpin;
GN   Name=ARPIN;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hippocampus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulates actin polymerization by inhibiting the actin-
CC       nucleating activity of the Arp2/3 complex; the function is competitive
CC       with nucleation promoting factors. Participates in an incoherent
CC       feedforward loop at the lamellipodium tip where it inhibits the ARP2/2
CC       complex in response to Rac signaling and where Rac also stimulates
CC       actin polymerization through the WAVE complex. Involved in steering
CC       cell migration by controlling its directional persistence (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Associates with the Arp2/3 complex. Interacts with ARPC2;
CC       enhanced by activated RAC1. Interacts with ARPC5; the interaction is
CC       dependent on RAC1 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, lamellipodium {ECO:0000250}.
CC       Note=Colocalized with the WAVE complex at lamelliupodium tip.
CC       {ECO:0000250}.
CC   -!- DOMAIN: The acidic C-terminus is necessary and sufficient to inhibit
CC       ARP2/3 complex activity. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Arpin family. {ECO:0000305}.
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DR   EMBL; BC118178; AAI18179.1; -; mRNA.
DR   RefSeq; NP_001069772.1; NM_001076304.1.
DR   AlphaFoldDB; Q17QU3; -.
DR   STRING; 9913.ENSBTAP00000038580; -.
DR   PaxDb; Q17QU3; -.
DR   PRIDE; Q17QU3; -.
DR   Ensembl; ENSBTAT00000038770; ENSBTAP00000038580; ENSBTAG00000027059.
DR   GeneID; 613997; -.
DR   KEGG; bta:613997; -.
DR   CTD; 348110; -.
DR   VEuPathDB; HostDB:ENSBTAG00000027059; -.
DR   VGNC; VGNC:108040; ARPIN.
DR   eggNOG; ENOG502R4IG; Eukaryota.
DR   GeneTree; ENSGT00530000064251; -.
DR   HOGENOM; CLU_106544_0_0_1; -.
DR   InParanoid; Q17QU3; -.
DR   OMA; HLMSSYK; -.
DR   OrthoDB; 1431350at2759; -.
DR   Proteomes; UP000009136; Chromosome 21.
DR   Bgee; ENSBTAG00000027059; Expressed in myometrium and 104 other tissues.
DR   ExpressionAtlas; Q17QU3; baseline.
DR   GO; GO:0030027; C:lamellipodium; ISS:UniProtKB.
DR   GO; GO:0033058; P:directional locomotion; ISS:UniProtKB.
DR   GO; GO:0051126; P:negative regulation of actin nucleation; ISS:UniProtKB.
DR   GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR   GO; GO:2000393; P:negative regulation of lamellipodium morphogenesis; ISS:UniProtKB.
DR   InterPro; IPR018889; Arpin.
DR   PANTHER; PTHR31199; PTHR31199; 1.
DR   Pfam; PF10574; UPF0552; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Reference proteome.
FT   CHAIN           1..226
FT                   /note="Arpin"
FT                   /id="PRO_0000327661"
FT   REGION          187..226
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          211..226
FT                   /note="Necessary and sufficient for interaction with ARPC2"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        195..211
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   226 AA;  24886 MW;  9A851BCB9EAEED5C CRC64;
     MSRIYQDSAL RNKAVRSARL PGAWDPAAHQ RGDGVLLEGE LLDVSRHSIL DAHGRKERYY
     VLYIRPSRIH RRKFDPKGNE IEPNFSATRK VNTGFLMSSY KVEAKGDSDR LTPEALKGLV
     NKPELLALTE SLTPAETVAF WMPESEMEAM ELELGAGVRL KTRGDGPFLD SLAKLEAGTV
     TKCNFAGDGK TGASWTDNIM AQKSSEGAAS ETREQGDGAA DEEWDD