ID   O1614_CONPE             Reviewed;          83 AA.
AC   Q9U657;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Mu-conotoxin-like PnMKLT1-014;
DE   Flags: Precursor;
OS   Conus pennaceus (Feathered cone) (Conus episcopus).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Darioconus.
OX   NCBI_TaxID=37335;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=11158371; DOI=10.1093/oxfordjournals.molbev.a003786;
RA   Conticello S.G., Gilad Y., Avidan N., Ben-Asher E., Levy Z., Fainzilber M.;
RT   "Mechanisms for evolving hypervariability: the case of conopeptides.";
RL   Mol. Biol. Evol. 18:120-131(2001).
CC   -!- FUNCTION: Mu-conotoxins block voltage-gated sodium channels (Nav).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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DR   EMBL; AF193259; AAF07970.1; -; mRNA.
DR   AlphaFoldDB; Q9U657; -.
DR   ConoServer; 1092; Pn6.14 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   2: Evidence at transcript level;
KW   Cleavage on pair of basic residues; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Signal; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..50
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000404742"
FT   PEPTIDE         53..83
FT                   /note="Mu-conotoxin-like PnMKLT1-014"
FT                   /id="PRO_0000404743"
FT   DISULFID        54..72
FT                   /evidence="ECO:0000250"
FT   DISULFID        61..77
FT                   /evidence="ECO:0000250"
FT   DISULFID        71..82
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   83 AA;  9259 MW;  4848C6A905649C09 CRC64;
     MNLTCMMIVA VLFLTAWTFV MADDSNNGLA NLFSKSRYEM EDPEPSKLEK RKTCQRRWDF
     CPGALVGVIT CCGGLICLGV MCI