ID   O161_CONEA              Reviewed;          69 AA.
AC   Q9N6A4;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Conotoxin Eb6.1;
DE   Flags: Precursor; Fragment;
GN   Name=E1;
OS   Conus ebraeus (Hebrew cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Virroconus.
OX   NCBI_TaxID=89425;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Guam, Hawaii, and Okinawa; TISSUE=Venom duct;
RX   PubMed=19606224; DOI=10.1371/journal.pone.0006245;
RA   Duda T.F. Jr., Chang D., Lewis B.D., Lee T.;
RT   "Geographic variation in venom allelic composition and diets of the
RT   widespread predatory marine gastropod Conus ebraeus.";
RL   PLoS ONE 4:E6245-E6245(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10958845; DOI=10.1093/oxfordjournals.molbev.a026412;
RA   Duda T.F. Jr., Palumbi S.R.;
RT   "Evolutionary diversification of multigene families: allelic selection of
RT   toxins in predatory cone snails.";
RL   Mol. Biol. Evol. 17:1286-1293(2000).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- MISCELLANEOUS: This precursor corresponds to allele E1a. Has not been
CC       merged with other alleles since they may differ due to geographic
CC       variation (see strains in PubMed:19606224).
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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DR   EMBL; AF174268; AAF89932.1; -; mRNA.
DR   EMBL; AF174271; AAF89935.1; -; mRNA.
DR   EMBL; AF174273; AAF89937.1; -; mRNA.
DR   EMBL; AF174274; AAF89938.1; -; mRNA.
DR   EMBL; AF174285; AAF89949.1; -; mRNA.
DR   EMBL; FJ804530; ACU56805.1; -; mRNA.
DR   AlphaFoldDB; Q9N6A4; -.
DR   ConoServer; 1242; Eb6.1 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          <1..17
FT                   /evidence="ECO:0000255"
FT   PROPEP          18..41
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000414632"
FT   PEPTIDE         42..69
FT                   /note="Conotoxin Eb6.1"
FT                   /id="PRO_0000414633"
FT   DISULFID        43..57
FT                   /evidence="ECO:0000250"
FT   DISULFID        50..61
FT                   /evidence="ECO:0000250"
FT   DISULFID        56..68
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   69 AA;  7695 MW;  ABF998A5DB20C4FA CRC64;
     VLIIAVLFLT ACQLTTAETY SRGRQKHRAR RSTDKNSKWT RECTHSGGAC NSHDQCCNAF
     CDTATRTCV