ARR12_ARATH
ID ARR12_ARATH Reviewed; 596 AA.
AC P62598; F4IRL1;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Two-component response regulator ARR12;
GN Name=ARR12; OrderedLocusNames=At2g25180; ORFNames=F13D4.140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 309-442.
RX PubMed=11115876; DOI=10.1104/pp.124.4.1582;
RA White J.A., Todd J., Newman T., Focks N., Girke T., de Ilarduya O.M.,
RA Jaworski J.G., Ohlrogge J.B., Benning C.;
RT "A new set of Arabidopsis expressed sequence tags from developing seeds.
RT The metabolic pathway from carbohydrates to seed oil.";
RL Plant Physiol. 124:1582-1594(2000).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=15173562; DOI=10.1104/pp.103.038109;
RA Mason M.G., Li J., Mathews D.E., Kieber J.J., Schaller G.E.;
RT "Type-B response regulators display overlapping expression patterns in
RT Arabidopsis.";
RL Plant Physiol. 135:927-937(2004).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=17363254; DOI=10.1016/j.cub.2007.02.047;
RA Dello Ioio R., Linhares F.S., Scacchi E., Casamitjana-Martinez E.,
RA Heidstra R., Costantino P., Sabatini S.;
RT "Cytokinins determine Arabidopsis root-meristem size by controlling cell
RT differentiation.";
RL Curr. Biol. 17:678-682(2007).
RN [6]
RP FUNCTION.
RX PubMed=20605455; DOI=10.1016/j.cub.2010.05.035;
RA Moubayidin L., Perilli S., Dello Ioio R., Di Mambro R., Costantino P.,
RA Sabatini S.;
RT "The rate of cell differentiation controls the Arabidopsis root meristem
RT growth phase.";
RL Curr. Biol. 20:1138-1143(2010).
CC -!- FUNCTION: Transcriptional activator that binds specifically to the DNA
CC sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in
CC His-to-Asp phosphorelay signal transduction system. Phosphorylation of
CC the Asp residue in the receiver domain activates the ability of the
CC protein to promote the transcription of target genes. Could directly
CC activate some type-A response regulators in response to cytokinins.
CC Involved in the root-meristem size determination through the regulation
CC of cell differentiation (PubMed:17363254). Involved in activating SHY2
CC during meristem growth and controls PIN expression via activation of
CC SHY2 (PubMed:20605455). {ECO:0000250|UniProtKB:Q940D0,
CC ECO:0000269|PubMed:17363254, ECO:0000269|PubMed:20605455}.
CC -!- SUBUNIT: Binds the target DNA as a monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Detected in the whole plant. Predominantly
CC expressed in leaves. Expressed at the root transition zone
CC (PubMed:17363254). {ECO:0000269|PubMed:15173562,
CC ECO:0000269|PubMed:17363254}.
CC -!- DEVELOPMENTAL STAGE: Detected immediately after seed germination.
CC {ECO:0000269|PubMed:17363254}.
CC -!- PTM: Two-component system major event consists of a His-to-Asp
CC phosphorelay between a sensor histidine kinase (HK) and a response
CC regulator (RR). In plants, the His-to-Asp phosphorelay involves an
CC additional intermediate named Histidine-containing phosphotransfer
CC protein (HPt). This multistep phosphorelay consists of a His-Asp-His-
CC Asp sequential transfer of a phosphate group between first an His and
CC an Asp of the HK protein, followed by the transfer to a conserved His
CC of the HPt protein and finally the transfer to an Asp in the receiver
CC domain of the RR protein.
CC -!- SIMILARITY: Belongs to the ARR family. Type-B subfamily. {ECO:0000305}.
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DR EMBL; CP002685; AEC07667.1; -; Genomic_DNA.
DR EMBL; BE522295; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_180090.6; NM_128075.7.
DR AlphaFoldDB; P62598; -.
DR SMR; P62598; -.
DR BioGRID; 2408; 8.
DR IntAct; P62598; 2.
DR STRING; 3702.AT2G25180.1; -.
DR PaxDb; P62598; -.
DR PRIDE; P62598; -.
DR ProteomicsDB; 246895; -.
DR EnsemblPlants; AT2G25180.1; AT2G25180.1; AT2G25180.
DR GeneID; 817056; -.
DR Gramene; AT2G25180.1; AT2G25180.1; AT2G25180.
DR KEGG; ath:AT2G25180; -.
DR Araport; AT2G25180; -.
DR TAIR; locus:2040194; AT2G25180.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_024359_0_0_1; -.
DR InParanoid; P62598; -.
DR OMA; MPLEFDQ; -.
DR OrthoDB; 332559at2759; -.
DR PRO; PR:P62598; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P62598; baseline and differential.
DR Genevisible; P62598; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000156; F:phosphorelay response regulator activity; ISS:TAIR.
DR GO; GO:1990110; P:callus formation; IGI:TAIR.
DR GO; GO:0071368; P:cellular response to cytokinin stimulus; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IGI:TAIR.
DR GO; GO:0010492; P:maintenance of shoot apical meristem identity; IGI:TAIR.
DR GO; GO:0080022; P:primary root development; IGI:TAIR.
DR GO; GO:0031537; P:regulation of anthocyanin metabolic process; IGI:TAIR.
DR GO; GO:0010380; P:regulation of chlorophyll biosynthetic process; IGI:TAIR.
DR GO; GO:0080036; P:regulation of cytokinin-activated signaling pathway; IMP:CACAO.
DR GO; GO:0010082; P:regulation of root meristem growth; IMP:CACAO.
DR GO; GO:0080113; P:regulation of seed growth; IMP:TAIR.
DR GO; GO:0009735; P:response to cytokinin; IGI:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IGI:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0048367; P:shoot system development; IGI:TAIR.
DR InterPro; IPR045279; ARR-like.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR006447; Myb_dom_plants.
DR InterPro; IPR017053; Response_reg_B-typ_pln.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR PANTHER; PTHR43874; PTHR43874; 1.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PIRSF; PIRSF036392; RR_ARR_type-B; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 2: Evidence at transcript level;
KW Activator; Cytokinin signaling pathway; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..596
FT /note="Two-component response regulator ARR12"
FT /id="PRO_0000132297"
FT DOMAIN 18..133
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 197..247
FT /note="Myb-like GARP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 138..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..467
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 194..197
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 147..165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 437..461
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 69
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 414
FT /note="G -> S (in Ref. 3; BE522295)"
FT /evidence="ECO:0000305"
FT CONFLICT 426
FT /note="H -> T (in Ref. 3; BE522295)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 596 AA; 65626 MW; C9E0D3F5EEE1B149 CRC64;
MTVEQNLEAL DQFPVGMRVL AVDDDQTCLK ILESLLRHCQ YHVTTTNQAQ KALELLRENK
NKFDLVISDV DMPDMDGFKL LELVGLEMDL PVIMLSAHSD PKYVMKGVTH GACDYLLKPV
RIEELKNIWQ HVVRSRFDKN RGSNNNGDKR DGSGNEGVGN SDQNNGKGNR KRKDQYNEDE
DEDRDDNDDS CAQKKQRVVW TVELHKKFVA AVNQLGYEKA MPKKILDLMN VEKLTRENVA
SHLQKFRLYL KRISGVANQQ AIMANSELHF MQMNGLDGFH HRPIPVGSGQ YHGGAPAMRS
FPPNGILGRL NTPSGIGVRS LSSPPAGMFL QNQTDIGKFH HVSSLPLNHS DGGNILQGLP
MPLEFDQLQT NNNKSRNMNS NKSIAGTSMA FPSFSTQQNS LISAPNNNVV VLEGHPQATP
PGFPGHQINK RLEHWSNAVS SSTHPPPPAH NSNSINHQFD VSPLPHSRPD PLEWNNVSSS
YSIPFCDSAN TLSSPALDTT NPRAFCRNTD FDSNTNVQPG VFYGPSTDAM ALLSSSNPKE
GFVVGQQKLQ SGGFMVADAG SLDDIVNSTM KQEQSQGDLS GGDLGYGGFS SLRTCI