ID   O163_CONLE              Reviewed;          75 AA.
AC   P0C904;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-APR-2009, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Conotoxin Leo-O3 {ECO:0000303|PubMed:18221274};
DE   Flags: Precursor;
OS   Conus leopardus (Leopard cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lithoconus.
OX   NCBI_TaxID=101306;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=18221274; DOI=10.1111/j.1365-294x.2007.03627.x;
RA   Remigio E.A., Duda T.F. Jr.;
RT   "Evolution of ecological specialization and venom of a predatory marine
RT   gastropod.";
RL   Mol. Ecol. 17:1156-1162(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:18221274}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:18221274}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EF467318; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0C904; -.
DR   ConoServer; 3719; Lp6.4 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   3: Inferred from homology;
KW   Amidation; Disulfide bond; Knottin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..42
FT                   /evidence="ECO:0000305|PubMed:18221274"
FT                   /id="PRO_0000368007"
FT   PEPTIDE         43..69
FT                   /note="Conotoxin Leo-O3"
FT                   /evidence="ECO:0000305|PubMed:18221274"
FT                   /id="PRO_0000368008"
FT   PROPEP          70..75
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000368009"
FT   MOD_RES         69
FT                   /note="Cysteine amide"
FT                   /evidence="ECO:0000250|UniProtKB:Q9XZK2"
FT   DISULFID        47..60
FT                   /evidence="ECO:0000305"
FT   DISULFID        54..63
FT                   /evidence="ECO:0000305"
FT   DISULFID        59..69
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   75 AA;  7832 MW;  086311BFB8656C32 CRC64;
     MKLTCVVIVA VLFLTACQLA TADISGGMRK HRALRSTTKL SRSPFDCSSP GAFCGLVPCC
     DSCNVLGRCG SGLHV