ARR14_ARATH
ID ARR14_ARATH Reviewed; 382 AA.
AC Q8L9Y3; Q9ZUA4;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Two-component response regulator ARR14;
GN Name=ARR14; OrderedLocusNames=At2g01760; ORFNames=T8O11.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=15173562; DOI=10.1104/pp.103.038109;
RA Mason M.G., Li J., Mathews D.E., Kieber J.J., Schaller G.E.;
RT "Type-B response regulators display overlapping expression patterns in
RT Arabidopsis.";
RL Plant Physiol. 135:927-937(2004).
CC -!- FUNCTION: Transcriptional activator that binds specifically to the DNA
CC sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in
CC His-to-Asp phosphorelay signal transduction system. Phosphorylation of
CC the Asp residue in the receiver domain activates the ability of the
CC protein to promote the transcription of target genes. Could directly
CC activate some type-A response regulators in response to cytokinins (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Binds the target DNA as a monomer. {ECO:0000250}.
CC -!- INTERACTION:
CC Q8L9Y3; Q9C5U2: AHK2; NbExp=3; IntAct=EBI-1100737, EBI-1100634;
CC Q8L9Y3; Q9ZNV9: AHP1; NbExp=2; IntAct=EBI-1100737, EBI-1100673;
CC Q8L9Y3; Q9ZNV8: AHP2; NbExp=3; IntAct=EBI-1100737, EBI-1100687;
CC Q8L9Y3; Q9SAZ5: AHP3; NbExp=3; IntAct=EBI-1100737, EBI-1100711;
CC Q8L9Y3; Q8L9Y3: ARR14; NbExp=2; IntAct=EBI-1100737, EBI-1100737;
CC Q8L9Y3; O80931: AS1; NbExp=3; IntAct=EBI-1100737, EBI-763232;
CC Q8L9Y3; Q67XQ1: At1g03430; NbExp=3; IntAct=EBI-1100737, EBI-1100725;
CC Q8L9Y3; O80575: At2g44050; NbExp=3; IntAct=EBI-1100737, EBI-4473692;
CC Q8L9Y3; Q17TI5: BRX; NbExp=3; IntAct=EBI-1100737, EBI-4426649;
CC Q8L9Y3; Q8VYD2: GPL1; NbExp=3; IntAct=EBI-1100737, EBI-4426914;
CC Q8L9Y3; Q38830: IAA12; NbExp=3; IntAct=EBI-1100737, EBI-617608;
CC Q8L9Y3; O24410: IAA20; NbExp=3; IntAct=EBI-1100737, EBI-632272;
CC Q8L9Y3; Q8LAL2: IAA26; NbExp=3; IntAct=EBI-1100737, EBI-3947418;
CC Q8L9Y3; Q9XFM0: IAA28; NbExp=3; IntAct=EBI-1100737, EBI-3133404;
CC Q8L9Y3; Q38822: IAA3; NbExp=3; IntAct=EBI-1100737, EBI-307174;
CC Q8L9Y3; Q9M1R4: IAA30; NbExp=3; IntAct=EBI-1100737, EBI-3946710;
CC Q8L9Y3; Q8H174: IAA31; NbExp=3; IntAct=EBI-1100737, EBI-3946408;
CC Q8L9Y3; O81313: IND; NbExp=3; IntAct=EBI-1100737, EBI-4446992;
CC Q8L9Y3; Q9FDW1: MYB44; NbExp=3; IntAct=EBI-1100737, EBI-15192813;
CC Q8L9Y3; O22179: MYB70; NbExp=5; IntAct=EBI-1100737, EBI-1238013;
CC Q8L9Y3; O23160: MYB73; NbExp=5; IntAct=EBI-1100737, EBI-25506855;
CC Q8L9Y3; O81127: RSZ21; NbExp=3; IntAct=EBI-1100737, EBI-927172;
CC Q8L9Y3; Q9LQF0: TCP23; NbExp=3; IntAct=EBI-1100737, EBI-15192297;
CC Q8L9Y3; Q84MB2: TIFY8; NbExp=6; IntAct=EBI-1100737, EBI-4426557;
CC Q8L9Y3; Q9SHZ6: UBA1A; NbExp=5; IntAct=EBI-1100737, EBI-346271;
CC Q8L9Y3; Q8GWF1: WRKY38; NbExp=3; IntAct=EBI-1100737, EBI-1993263;
CC Q8L9Y3; Q9SK33: WRKY60; NbExp=3; IntAct=EBI-1100737, EBI-2112777;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in young leaf tissue.
CC {ECO:0000269|PubMed:15173562}.
CC -!- PTM: Two-component system major event consists of a His-to-Asp
CC phosphorelay between a sensor histidine kinase (HK) and a response
CC regulator (RR). In plants, the His-to-Asp phosphorelay involves an
CC additional intermediate named Histidine-containing phosphotransfer
CC protein (HPt). This multistep phosphorelay consists of a His-Asp-His-
CC Asp sequential transfer of a phosphate group between first an His and
CC an Asp of the HK protein, followed by the transfer to a conserved His
CC of the HPt protein and finally the transfer to an Asp in the receiver
CC domain of the RR protein.
CC -!- SIMILARITY: Belongs to the ARR family. Type-B subfamily. {ECO:0000305}.
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DR EMBL; AC006069; AAD12696.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05495.1; -; Genomic_DNA.
DR EMBL; AY099726; AAM20577.1; -; mRNA.
DR EMBL; AY128887; AAM91287.1; -; mRNA.
DR EMBL; AY088149; AAM65694.1; -; mRNA.
DR PIR; G84428; G84428.
DR RefSeq; NP_178285.1; NM_126237.3.
DR AlphaFoldDB; Q8L9Y3; -.
DR SMR; Q8L9Y3; -.
DR BioGRID; 110; 76.
DR IntAct; Q8L9Y3; 75.
DR STRING; 3702.AT2G01760.1; -.
DR PaxDb; Q8L9Y3; -.
DR PRIDE; Q8L9Y3; -.
DR EnsemblPlants; AT2G01760.1; AT2G01760.1; AT2G01760.
DR GeneID; 814707; -.
DR Gramene; AT2G01760.1; AT2G01760.1; AT2G01760.
DR KEGG; ath:AT2G01760; -.
DR Araport; AT2G01760; -.
DR TAIR; locus:2065398; AT2G01760.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_061273_2_1_1; -.
DR InParanoid; Q8L9Y3; -.
DR PhylomeDB; Q8L9Y3; -.
DR PRO; PR:Q8L9Y3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8L9Y3; baseline and differential.
DR Genevisible; Q8L9Y3; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000156; F:phosphorelay response regulator activity; ISS:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; TAS:TAIR.
DR InterPro; IPR045279; ARR-like.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR006447; Myb_dom_plants.
DR InterPro; IPR017053; Response_reg_B-typ_pln.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR PANTHER; PTHR43874; PTHR43874; 1.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PIRSF; PIRSF036392; RR_ARR_type-B; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Cytokinin signaling pathway; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..382
FT /note="Two-component response regulator ARR14"
FT /id="PRO_0000132299"
FT DOMAIN 12..128
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 202..252
FT /note="Myb-like GARP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 171..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 199..202
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOD_RES 63
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 354
FT /note="D -> N (in Ref. 4; AAM65694)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 382 AA; 43099 MW; 0374BB7AF011CE49 CRC64;
MPINDQFPSG LRILVVDDDT SCLFILEKML LRLMYQVTIC SQADVALTIL RERKDSFDLV
LSDVHMPGMN GYNLLQQVGL LEMDLPVIMM SVDGRTTTVM TGINHGACDY LIKPIRPEEL
KNIWQHVVRR KCVMKKELRS SQALEDNKNS GSLETVVVSV SECSEESLMK CRNKKKKKKR
SVDRDDNEDD LLLDPGNSKK SRVVWSIELH QQFVNAVNKL GIDKAVPKRI LELMNVPGLS
RENVASHLQK FRLYLKRLSG EASQSNDSES TKRYENIQAL VSSGQLHPQT LAALFGQPID
NHHSASFGVW IPNDNLGRSQ NEHFSVDVSS ASNRPVSVAV HGLSSSANFR QRGDVNNNRI
RQGYGSNVNE ESWILERSSR QR
