ID   O164_CONBE              Reviewed;          84 AA.
AC   M9PQ91;
DT   10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2013, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Delta-conotoxin-like Bt6.4 {ECO:0000312|EMBL:AGG19140.1};
DE   Flags: Precursor;
OS   Conus betulinus (Beech cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Dendroconus.
OX   NCBI_TaxID=89764;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Wu C., Liu Z., Dai Q.;
RL   Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This toxin activates voltage-gated sodium channels. It shifts
CC       the voltage-dependence of activation to more hyperpolarized potentials
CC       but has only little effect on channel inactivation. It is active on
CC       Nav1.3/SCN3A (EC(50)=3.98 nM), Nav1.4/SCN4A (EC(50)=4.99 nM),
CC       Nav1.6/SCN8A (EC(50)=1.27 nM) and Nav1.7/SCN9A (EC(50)=2.42 nM)
CC       voltage-gated sodium channels. In vivo, it induces nocifensive or pain-
CC       like behaviors in mice when injected intraplantarly.
CC       {ECO:0000250|UniProtKB:P0DL67}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P0DL66}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P60513}.
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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DR   EMBL; JX000438; AGG19140.1; -; mRNA.
DR   AlphaFoldDB; M9PQ91; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   3: Inferred from homology;
KW   Cleavage on pair of basic residues; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Signal; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..57
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000439830"
FT   PEPTIDE         58..84
FT                   /note="Delta-conotoxin-like Bt6.4"
FT                   /evidence="ECO:0000250|UniProtKB:A0A0F6QDA5"
FT                   /id="PRO_5004101955"
FT   DISULFID        58..74
FT                   /evidence="ECO:0000250|UniProtKB:P60513"
FT   DISULFID        65..78
FT                   /evidence="ECO:0000250|UniProtKB:P60513"
FT   DISULFID        73..82
FT                   /evidence="ECO:0000250|UniProtKB:P60513"
SQ   SEQUENCE   84 AA;  9151 MW;  38C8AEB0A7B9E953 CRC64;
     MKLTCMVIVA VLFLTAWTSV MADGSINRPD IAEGWQKFFS KARDEMKNRA ASELNKRCAG
     IGSFCGLPGL VDCCSGRCFI VCLP