ID   O164_CONBU              Reviewed;          74 AA.
AC   P0CY63;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Conotoxin Bu4;
DE   Flags: Precursor;
OS   Conus bullatus (Bubble cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Textilia.
OX   NCBI_TaxID=89438;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=21266071; DOI=10.1186/1471-2164-12-60;
RA   Hu H., Bandyopadhyay P.K., Olivera B.M., Yandell M.;
RT   "Characterization of the Conus bullatus genome and its venom-duct
RT   transcriptome.";
RL   BMC Genomics 12:60-60(2011).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0CY63; -.
DR   SMR; P0CY63; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..45
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000409941"
FT   PEPTIDE         46..74
FT                   /note="Conotoxin Bu4"
FT                   /id="PRO_0000409942"
FT   DISULFID        47..63
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        62..73
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   74 AA;  8230 MW;  642B2D02DE246492 CRC64;
     MKLTCVVIVA VLLLTACQLI IAEDSRGTQL HRALRKATKL SVSTRTCVMF GSMCDKEEHS
     ICCYECDYKK GICV