ID   O164_CONVR              Reviewed;          79 AA.
AC   P0DW75;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Conotoxin Vi6.4 {ECO:0000303|PubMed:26851775};
DE   Flags: Precursor;
OS   Conus virgo (Virgin cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Virgiconus.
OX   NCBI_TaxID=89427;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=26851775; DOI=10.1016/j.toxicon.2016.02.001;
RA   Espino S.S., Dilanyan T., Imperial J.S., Aguilar M.B., Teichert R.W.,
RA   Bandyopadhyay P., Olivera B.M.;
RT   "Glycine-rich conotoxins from the Virgiconus clade.";
RL   Toxicon 113:11-17(2016).
CC   -!- FUNCTION: Ion channel inhibitor that inhibits the increase in
CC       intracellular calcium upon depolarization in DRG neurons. In vivo, both
CC       intraperitoneal and intracranial injections into mice induce
CC       hyperactivity. {ECO:0000250|UniProtKB:Q5K0C7}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:26851775}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:26851775}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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PE   3: Inferred from homology;
KW   Disulfide bond; Hydroxylation; Ion channel impairing toxin; Knottin;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..47
FT                   /evidence="ECO:0000250|UniProtKB:Q5K0C7"
FT                   /id="PRO_0000456326"
FT   PEPTIDE         48..79
FT                   /note="Conotoxin Vi6.4"
FT                   /evidence="ECO:0000250|UniProtKB:Q5K0C7"
FT                   /id="PRO_0000456327"
FT   MOD_RES         60
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000250|UniProtKB:Q5K0C7"
FT   MOD_RES         63
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000250|UniProtKB:Q5K0C7"
FT   DISULFID        49..62
FT                   /evidence="ECO:0000250|UniProtKB:P60179"
FT   DISULFID        56..67
FT                   /evidence="ECO:0000250|UniProtKB:P60179"
FT   DISULFID        61..77
FT                   /evidence="ECO:0000250|UniProtKB:P60179"
SQ   SEQUENCE   79 AA;  8531 MW;  4E5710AE478FD030 CRC64;
     MKLTCVLIIT VLFLTASQLI TADYSGDKRQ YRAVRLRDEM RNFKGARDCG EQGQGCYTRP
     CCPGLHCAAG ATGGGSCQP