ID   O168_CONVX              Reviewed;          79 AA.
AC   Q5K0C8;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Conotoxin 8;
DE   Flags: Precursor;
OS   Conus vexillum (Flag cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Rhizoconus.
OX   NCBI_TaxID=89431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=15652641; DOI=10.1016/j.peptides.2004.10.027;
RA   Kauferstein S., Melaun C., Mebs D.;
RT   "Direct cDNA cloning of novel conopeptide precursors of the O-
RT   superfamily.";
RL   Peptides 26:361-367(2005).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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DR   EMBL; AJ851180; CAH64853.1; -; mRNA.
DR   AlphaFoldDB; Q5K0C8; -.
DR   SMR; Q5K0C8; -.
DR   ConoServer; 1069; Conotoxin-8 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..47
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000035005"
FT   PEPTIDE         48..79
FT                   /note="Conotoxin 8"
FT                   /id="PRO_0000035006"
FT   DISULFID        49..62
FT                   /evidence="ECO:0000250"
FT   DISULFID        56..67
FT                   /evidence="ECO:0000250"
FT   DISULFID        61..77
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   79 AA;  8450 MW;  9FC947620C4531E4 CRC64;
     MKLTCVLIIT VLFLTASQLI TADYSRGQRQ YRAVRLGDEM RNFKGARDCG GQGEGCYTQP
     CCPGLRCRGG GTGGGSCQP