ID   O16A_CONVX              Reviewed;          74 AA.
AC   Q86RA3;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Conotoxin VxVIA;
DE   AltName: Full=Conotoxin vx6a;
DE   Flags: Precursor;
OS   Conus vexillum (Flag cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Rhizoconus.
OX   NCBI_TaxID=89431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-74, SYNTHESIS OF 48-74,
RP   FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=16457862; DOI=10.1016/j.toxicon.2005.12.007;
RA   Jiang H., Xu C.-Q., Wang C.-Z., Fan C.-X., Zhao T.-Y., Chen J.-S.,
RA   Chi C.-W.;
RT   "Two novel O-superfamily conotoxins from Conus vexillum.";
RL   Toxicon 47:425-436(2006).
CC   -!- FUNCTION: When injected intracranially in mice, induces a series of
CC       symptoms such as quivering, climbing, scratching, barrel rolling and
CC       paralysis of limbs. Unexpectedly, no effect is observed on ionic
CC       currents when tested on locust DUM neuron.
CC       {ECO:0000269|PubMed:16457862}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- MASS SPECTROMETRY: Mass=2775.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16457862};
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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DR   EMBL; AF548000; AAO21703.1; -; mRNA.
DR   AlphaFoldDB; Q86RA3; -.
DR   SMR; Q86RA3; -.
DR   ConoServer; 824; VxVIA precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   InterPro; IPR018072; Conotoxin_a-typ_CS.
DR   Pfam; PF02950; Conotoxin; 1.
DR   PROSITE; PS60014; ALPHA_CONOTOXIN; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Knottin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..47
FT                   /evidence="ECO:0000269|PubMed:16457862"
FT                   /id="PRO_0000234823"
FT   PEPTIDE         48..74
FT                   /note="Conotoxin VxVIA"
FT                   /id="PRO_0000234824"
FT   DISULFID        48..62
FT                   /evidence="ECO:0000250"
FT   DISULFID        55..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        61..73
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   74 AA;  8118 MW;  3ED38876EC76E459 CRC64;
     MKLTCVLIIA VLFLTAYQLA TAASHAKGKQ KHRALRPADK HFRFTKRCNN RGGGCSQHPH
     CCSGTCNKTF GVCL