ID   O17A_CONCF              Reviewed;          31 AA.
AC   P0C1C8;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Gamma-conotoxin-like As7a;
OS   Conus cancellatus (Cancellate cone) (Conus austini).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Dauciconus.
OX   NCBI_TaxID=289020;
RN   [1]
RP   PROTEIN SEQUENCE, GAMMA-CARBOXYGLUTAMATION AT GLU-14, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=16325964; DOI=10.1016/j.peptides.2005.07.021;
RA   Zugasti-Cruz A., Maillo M., Lopez-Vera E., Falcon A.,
RA   Heimer de la Cotera E.P., Olivera B.M., Aguilar M.B.;
RT   "Amino acid sequence and biological activity of a gamma-conotoxin-like
RT   peptide from the worm-hunting snail Conus austini.";
RL   Peptides 27:506-511(2006).
CC   -!- FUNCTION: Gamma-conotoxins may act on voltage-gated non-specific cation
CC       pacemaker channels (HCN) (By similarity). Elicits toxic effects in the
CC       freshwater snail Pomacea paludosa after intramuscular injection, but it
CC       has no effect when injected intracerebrally into mice. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- MASS SPECTROMETRY: Mass=3236.16; Method=MALDI; Note=Decarboxylated.;
CC       Evidence={ECO:0000269|PubMed:16325964};
CC   -!- SIMILARITY: Belongs to the conotoxin O1 superfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C1C8; -.
DR   SMR; P0C1C8; -.
DR   ConoServer; 1473; AsVIIA.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Gamma-carboxyglutamic acid;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..31
FT                   /note="Gamma-conotoxin-like As7a"
FT                   /id="PRO_0000234827"
FT   MOD_RES         14
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:16325964"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000250"
FT   DISULFID        9..20
FT                   /evidence="ECO:0000250"
FT   DISULFID        15..31
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   31 AA;  3245 MW;  BCD55A992F2F0281 CRC64;
     TCKQKGEGCS LDVECCSSSC KPGGPLFDFD C