ID   O262_CONIM              Reviewed;          90 AA.
AC   A0A125S9F2; M9PQB5;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   13-APR-2016, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Conotoxin Im6.2 {ECO:0000312|EMBL:AGG19141.1};
DE   AltName: Full=Conopeptide im018 {ECO:0000303|PubMed:30893765, ECO:0000312|EMBL:AME17676.1};
DE   Flags: Precursor;
OS   Conus imperialis (Imperial cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Stephanoconus.
OX   NCBI_TaxID=35631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=30893765; DOI=10.3390/md17030177;
RA   Jin A.H., Dutertre S., Dutt M., Lavergne V., Jones A., Lewis R.J.,
RA   Alewood P.F.;
RT   "Transcriptomic-proteomic correlation in the predation-evoked venom of the
RT   cone snail, Conus imperialis.";
RL   Mar. Drugs 17:0-0(2019).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Wu C., Liu Z., Dai Q.;
RL   Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable neurotoxin. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30893765}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:30893765}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the conotoxin O2 superfamily. {ECO:0000305}.
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DR   EMBL; KT377412; AME17676.1; -; mRNA.
DR   EMBL; JX000439; AGG19141.1; -; mRNA.
DR   AlphaFoldDB; A0A125S9F2; -.
DR   SMR; A0A125S9F2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Disulfide bond; Neurotoxin;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   PROPEP          19..29
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000451004"
FT   CHAIN           30..89
FT                   /note="Conotoxin Im6.2"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000451032"
FT   MOD_RES         89
FT                   /note="Glutamic acid 1-amide"
FT                   /evidence="ECO:0000305"
FT   DISULFID        61..75
FT                   /evidence="ECO:0000305"
FT   DISULFID        68..79
FT                   /evidence="ECO:0000305"
FT   DISULFID        74..84
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1
FT                   /note="M -> ME (in Ref. 2; AGG19141)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   90 AA;  10536 MW;  3DD2EC2E7BB72AEB CRC64;
     MKLTILLLVA ALLVLTQART ERRRVKSRKT SSTYDDEMAT FCWSYWNEFQ YSYPYTYVQP
     CLTLGKACTT NSDCCSKYCN TKMCKINWEG