O26_CONTE
ID O26_CONTE Reviewed; 70 AA.
AC Q9BPA9;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 47.
DE RecName: Full=TxMEKL-P2 {ECO:0000303|PubMed:23031820, ECO:0000312|EMBL:AAG60456.1};
DE AltName: Full=Conotoxin 6 {ECO:0000305};
DE Flags: Precursor;
OS Conus textile (Cloth-of-gold cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX NCBI_TaxID=6494;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11158371; DOI=10.1093/oxfordjournals.molbev.a003786;
RA Conticello S.G., Gilad Y., Avidan N., Ben-Asher E., Levy Z., Fainzilber M.;
RT "Mechanisms for evolving hypervariability: the case of conopeptides.";
RL Mol. Biol. Evol. 18:120-131(2001).
RN [2]
RP PROTEIN SEQUENCE OF 42-70, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP BROMINATION AT TRP-60.
RC TISSUE=Venom;
RX PubMed=19380747; DOI=10.1073/pnas.0900745106;
RA Ueberheide B.M., Fenyo D., Alewood P.F., Chait B.T.;
RT "Rapid sensitive analysis of cysteine rich peptide venom components.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6910-6915(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, HYDROXYLATION AT
RP PRO-49, AND BROMINATION AT TRP-59 AND TRP-60.
RC TISSUE=Venom;
RX PubMed=22709442; DOI=10.1021/pr300312h;
RA Bhatia S., Kil Y.J., Ueberheide B., Chait B.T., Tayo L., Cruz L., Lu B.,
RA Yates J.R. III, Bern M.;
RT "Constrained de novo sequencing of conotoxins.";
RL J. Proteome Res. 11:4191-4200(2012).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, POSITION IN VENOM
RP DUCT, AND BROMINATION AT TRP-59 AND TRP-60.
RC TISSUE=Venom;
RX PubMed=23031820; DOI=10.1016/j.toxicon.2012.09.013;
RA Dobson R., Collodoro M., Gilles N., Turtoi A., De Pauw E., Quinton L.;
RT "Secretion and maturation of conotoxins in the venom ducts of Conus
RT textile.";
RL Toxicon 60:1370-1379(2012).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19380747}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct. All three forms are
CC mostly present in part 5 of venom duct (distal part near the pharynx)
CC and less abundantly present in part 4. {ECO:0000305|PubMed:19380747,
CC ECO:0000305|PubMed:23031820}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:P0C834}.
CC -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC -!- PTM: Contains 3 disulfide bonds. {ECO:0000269|PubMed:19380747}.
CC -!- PTM: Exists in 5 different forms, depending on bromination and
CC hydroxylation. Form P2 does not contain brominated tryptophan
CC (PubMed:23031820), form P2-WBr has one brominated tryptophan at
CC position 60 (PubMed:22709442, PubMed:23031820), form P2-2WBr has two
CC brominated tryptophans at position 59 and 60 (PubMed:23031820), form
CC hydroxyPro-P2-WBr has a hydroxyproline at position 49 and a brominated
CC tryptophan at position 59 (PubMed:22709442), and form hydroxyPro-P2-
CC 2WBr has an hydroxyproline at position 49 and two brominated tryptophan
CC at position 59 and 60 (PubMed:22709442). {ECO:0000269|PubMed:22709442,
CC ECO:0000269|PubMed:23031820}.
CC -!- MASS SPECTROMETRY: Mass=3302.092; Mass_error=0.02; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:19380747};
CC -!- SIMILARITY: Belongs to the conotoxin O2 superfamily. {ECO:0000305}.
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DR EMBL; AF215028; AAG60456.1; -; mRNA.
DR AlphaFoldDB; Q9BPA9; -.
DR SMR; Q9BPA9; -.
DR ConoServer; 715; TxMEKL-P2 precursor.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR004214; Conotoxin.
DR Pfam; PF02950; Conotoxin; 1.
PE 1: Evidence at protein level;
KW Bromination; Direct protein sequencing; Disulfide bond; Hydroxylation;
KW Secreted; Signal; Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..41
FT /evidence="ECO:0000305"
FT /id="PRO_0000371265"
FT PEPTIDE 42..70
FT /note="TxMEKL-P2"
FT /evidence="ECO:0000269|PubMed:19380747"
FT /id="PRO_0000371266"
FT MOD_RES 49
FT /note="4-hydroxyproline; partial"
FT /evidence="ECO:0000269|PubMed:22709442"
FT MOD_RES 59
FT /note="6'-bromotryptophan; partial"
FT /evidence="ECO:0000269|PubMed:22709442,
FT ECO:0000269|PubMed:23031820"
FT MOD_RES 60
FT /note="6'-bromotryptophan; partial"
FT /evidence="ECO:0000269|PubMed:19380747,
FT ECO:0000269|PubMed:22709442, ECO:0000269|PubMed:23031820"
FT DISULFID 43..57
FT /evidence="ECO:0000250|UniProtKB:P0C834"
FT DISULFID 50..62
FT /evidence="ECO:0000250|UniProtKB:P0C834"
FT DISULFID 56..69
FT /evidence="ECO:0000250|UniProtKB:P0C834"
SQ SEQUENCE 70 AA; 7701 MW; C7B96B8E5ECE45AF CRC64;
MEKLTILLLV AAVLTSTQAL IQGGGDERQK AKINFLSRSD RDCRGYDAPC SSGAPCCDWW
TCSARTNRCF