ID   O27A_CONPE              Reviewed;          80 AA.
AC   P56711; Q9BPA8;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Gamma-conotoxin PnVIIA;
DE   AltName: Full=PnMEKL-011;
DE   Flags: Precursor;
OS   Conus pennaceus (Feathered cone) (Conus episcopus).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Darioconus.
OX   NCBI_TaxID=37335;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11158371; DOI=10.1093/oxfordjournals.molbev.a003786;
RA   Conticello S.G., Gilad Y., Avidan N., Ben-Asher E., Levy Z., Fainzilber M.;
RT   "Mechanisms for evolving hypervariability: the case of conopeptides.";
RL   Mol. Biol. Evol. 18:120-131(2001).
RN   [2]
RP   PROTEIN SEQUENCE OF 46-77, FUNCTION, HYDROXYLATION AT PRO-76,
RP   GAMMA-CARBOXYGLUTAMATION AT GLU-59 AND GLU-71, SUBCELLULAR LOCATION, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=9484216; DOI=10.1021/bi971571f;
RA   Fainzilber M., Nakamura T., Lodder J.C., Zlotkin E., Kits K.S.,
RA   Burlingame A.L.;
RT   "Gamma-conotoxin-PnVIIA, a gamma-carboxyglutamate-containing peptide
RT   agonist of neuronal pacemaker cation currents.";
RL   Biochemistry 37:1470-1477(1998).
CC   -!- FUNCTION: Gamma-conotoxins may act on voltage-gated non-specific cation
CC       pacemaker channels (HCN). Triggers depolarization and firing of action
CC       potential bursts in the caudodorsal neurons of lymnaea. This effect is
CC       due to activation or enhancement of a slow inward cation current that
CC       may underlie endogenous bursting activity of these neurons.
CC       {ECO:0000269|PubMed:9484216}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9484216}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:9484216}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=3718.4; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:9484216};
CC   -!- SIMILARITY: Belongs to the conotoxin O2 superfamily. {ECO:0000305}.
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DR   EMBL; AF215029; AAG60457.1; -; mRNA.
DR   AlphaFoldDB; P56711; -.
DR   SMR; P56711; -.
DR   TCDB; 8.B.16.2.1; the maurocalcine (maca) family.
DR   ConoServer; 1636; PnVIIA.
DR   ConoServer; 716; PnVIIA precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Gamma-carboxyglutamic acid; Hydroxylation;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..43
FT                   /evidence="ECO:0000305|PubMed:9484216"
FT                   /id="PRO_0000392707"
FT   PEPTIDE         46..77
FT                   /note="Gamma-conotoxin PnVIIA"
FT                   /evidence="ECO:0000269|PubMed:9484216"
FT                   /id="PRO_0000044877"
FT   PROPEP          78..80
FT                   /evidence="ECO:0000305|PubMed:9484216"
FT                   /id="PRO_0000392708"
FT   MOD_RES         59
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:9484216"
FT   MOD_RES         71
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:9484216"
FT   MOD_RES         76
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:9484216"
FT   DISULFID        47..61
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..65
FT                   /evidence="ECO:0000250"
FT   DISULFID        60..70
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   80 AA;  9189 MW;  758ACDD9692E18D7 CRC64;
     MEKLTILLLV AAVLMSTQAQ NQEQRQQAKI NFLSKRKPSA ERWRRDCTSW FGRCTVNSEC
     CSNSCDQTYC ELYAFPSFGA