ID   O27A_CONTE              Reviewed;          76 AA.
AC   P24160; Q9BPB5; Q9BPB6;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 3.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Gamma-conotoxin-like TxVIIA {ECO:0000303|PubMed:8868490};
DE   AltName: Full=TxIIA {ECO:0000303|PubMed:1761058};
DE   Flags: Precursor;
OS   Conus textile (Cloth-of-gold cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX   NCBI_TaxID=6494;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11158371; DOI=10.1093/oxfordjournals.molbev.a003786;
RA   Conticello S.G., Gilad Y., Avidan N., Ben-Asher E., Levy Z., Fainzilber M.;
RT   "Mechanisms for evolving hypervariability: the case of conopeptides.";
RL   Mol. Biol. Evol. 18:120-131(2001).
RN   [2]
RP   PROTEIN SEQUENCE OF 49-75, GAMMA-CARBOXYGLUTAMATION AT GLU-57 AND GLU-61,
RP   AND SUBCELLULAR LOCATION.
RC   STRAIN=Neovicarius; TISSUE=Venom;
RX   PubMed=1761058; DOI=10.1111/j.1432-1033.1991.tb16412.x;
RA   Fainzilber M., Gordon D., Hasson A., Spira M.E., Zlotkin E.;
RT   "Mollusc-specific toxins from the venom of Conus textile neovicarius.";
RL   Eur. J. Biochem. 202:589-595(1991).
RN   [3]
RP   SEQUENCE REVISION TO 49; 75 AND 76, AMIDATION AT PHE-75, AND MASS
RP   SPECTROMETRY.
RX   PubMed=8868490; DOI=10.1002/pro.5560050315;
RA   Nakamura T., Yu Z., Fainzilber M., Burlingame A.L.;
RT   "Mass spectrometric-based revision of the structure of a cysteine-rich
RT   peptide toxin with gamma-carboxyglutamic acid, TxVIIA, from the sea snail,
RT   Conus textile.";
RL   Protein Sci. 5:524-530(1996).
CC   -!- FUNCTION: Gamma-conotoxins may act on voltage-gated non-specific cation
CC       pacemaker channels (HCN) (By similarity). Potent neurotoxin.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1761058}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:1761058}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=3088.9; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:8868490};
CC   -!- SIMILARITY: Belongs to the conotoxin O2 superfamily. {ECO:0000305}.
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DR   EMBL; AF215019; AAG60447.1; -; mRNA.
DR   EMBL; AF215020; AAG60448.1; -; mRNA.
DR   PIR; A58175; A58175.
DR   AlphaFoldDB; P24160; -.
DR   ConoServer; 1517; TxVIIA.
DR   ConoServer; 706; TxVIIA precursor.
DR   ConoServer; 707; TxVIIA precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Gamma-carboxyglutamic acid; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..46
FT                   /evidence="ECO:0000269|PubMed:1761058"
FT                   /id="PRO_0000392713"
FT   PEPTIDE         49..75
FT                   /note="Gamma-conotoxin-like TxVIIA"
FT                   /evidence="ECO:0000269|PubMed:1761058"
FT                   /id="PRO_0000044485"
FT   MOD_RES         57
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:1761058"
FT   MOD_RES         61
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:1761058"
FT   MOD_RES         75
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:8868490"
FT   DISULFID        49..63
FT                   /evidence="ECO:0000250"
FT   DISULFID        56..67
FT                   /evidence="ECO:0000250"
FT   DISULFID        62..72
FT                   /evidence="ECO:0000250"
FT   CONFLICT        23..25
FT                   /note="Missing (in Ref. 1; AAG60448)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   76 AA;  8529 MW;  197F229C146A475B CRC64;
     MEKLTILLLV AAVLMSTQAM FQGDGEKSRK AEINFSETRK LARNKQKRCG GYSTYCEVDS
     ECCSDNCVRS YCTLFG