O2AT4_HUMAN
ID O2AT4_HUMAN Reviewed; 320 AA.
AC A6NND4; B9EGZ8;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Olfactory receptor 2AT4;
DE AltName: Full=Olfactory receptor OR11-265;
GN Name=OR2AT4 {ECO:0000303|PubMed:24999593, ECO:0000312|HGNC:HGNC:19620};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION.
RX PubMed=14983052; DOI=10.1073/pnas.0307882100;
RA Malnic B., Godfrey P.A., Buck L.B.;
RT "The human olfactory receptor gene family.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:2584-2589(2004).
RN [4]
RP ERRATUM OF PUBMED:14983052.
RA Malnic B., Godfrey P.A., Buck L.B.;
RL Proc. Natl. Acad. Sci. U.S.A. 101:7205-7205(2004).
RN [5]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=24999593; DOI=10.1038/jid.2014.273;
RA Busse D., Kudella P., Gruening N.M., Gisselmann G., Staender S., Luger T.,
RA Jacobsen F., Steinstraesser L., Paus R., Gkogkolou P., Boehm M., Hatt H.,
RA Benecke H.;
RT "A synthetic sandalwood odorant induces wound-healing processes in human
RT keratinocytes via the olfactory receptor OR2AT4.";
RL J. Invest. Dermatol. 134:2823-2832(2014).
RN [6]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=30228264; DOI=10.1038/s41467-018-05973-0;
RA Cheret J., Bertolini M., Ponce L., Lehmann J., Tsai T., Alam M., Hatt H.,
RA Paus R.;
RT "Olfactory receptor OR2AT4 regulates human hair growth.";
RL Nat. Commun. 9:3624-3624(2018).
CC -!- FUNCTION: Olfactory receptor (PubMed:24999593, PubMed:30228264).
CC Activated by the synthetic sandalwood odorant sandalore
CC (PubMed:24999593, PubMed:30228264). Endogenous ligand is unknown
CC (Probable). The activity of this receptor is probably mediated by G
CC proteins which induce elevation of intracellular Ca(2+), a cAMP-
CC dependent pathway and phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and p38
CC MAPKs (PubMed:24999593, PubMed:30228264). Activation of OR2AT4 induces
CC proliferation, migration, and re-epithelialization during wound-healing
CC processes of keratinocytes (PubMed:24999593). Stimulation of OR2AT4 by
CC sandalore promotes hair growth by decreasing apoptosis and increasing
CC production of the anagen-prolonging growth factor IGF1 as well as other
CC pathways involving various kinases (PubMed:30228264).
CC {ECO:0000269|PubMed:24999593, ECO:0000269|PubMed:30228264,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24999593};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Detected in the keratinocytes of the epidermis (at
CC protein level) (PubMed:24999593). Detected in hair follicles in
CC proximal outer root sheath and hair matrix keratinocytes (at protein
CC level) (PubMed:30228264). {ECO:0000269|PubMed:24999593,
CC ECO:0000269|PubMed:30228264}.
CC -!- INDUCTION: Down-regulated during spontaneous, apoptosis-driven hair
CC follicles regression (catagen). {ECO:0000269|PubMed:30228264}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- WEB RESOURCE: Name=Human Olfactory Receptor Data Exploratorium (HORDE);
CC URL="http://genome.weizmann.ac.il/horde/card/index/symbol:OR2AT4";
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DR EMBL; AP003175; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC136958; AAI36959.1; -; mRNA.
DR EMBL; BC136959; AAI36960.1; -; mRNA.
DR EMBL; BK004820; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS31639.1; -.
DR RefSeq; NP_001005285.1; NM_001005285.1.
DR RefSeq; XP_016873144.1; XM_017017655.1.
DR RefSeq; XP_016873145.1; XM_017017656.1.
DR RefSeq; XP_016873146.1; XM_017017657.1.
DR RefSeq; XP_016873147.1; XM_017017658.1.
DR AlphaFoldDB; A6NND4; -.
DR SMR; A6NND4; -.
DR STRING; 9606.ENSP00000304846; -.
DR GlyGen; A6NND4; 1 site.
DR PhosphoSitePlus; A6NND4; -.
DR BioMuta; OR2AT4; -.
DR PaxDb; A6NND4; -.
DR PeptideAtlas; A6NND4; -.
DR PRIDE; A6NND4; -.
DR Antibodypedia; 56326; 101 antibodies from 20 providers.
DR DNASU; 341152; -.
DR Ensembl; ENST00000305159.3; ENSP00000304846.3; ENSG00000171561.4.
DR Ensembl; ENST00000641504.1; ENSP00000493318.1; ENSG00000171561.4.
DR Ensembl; ENST00000641541.1; ENSP00000493299.1; ENSG00000171561.4.
DR Ensembl; ENST00000641593.1; ENSP00000493416.1; ENSG00000171561.4.
DR Ensembl; ENST00000641931.1; ENSP00000493460.1; ENSG00000171561.4.
DR GeneID; 341152; -.
DR KEGG; hsa:341152; -.
DR UCSC; uc010rro.3; human.
DR CTD; 341152; -.
DR GeneCards; OR2AT4; -.
DR HGNC; HGNC:19620; OR2AT4.
DR HPA; ENSG00000171561; Tissue enhanced (brain, retina).
DR neXtProt; NX_A6NND4; -.
DR PharmGKB; PA134941860; -.
DR VEuPathDB; HostDB:ENSG00000171561; -.
DR eggNOG; ENOG502SJUD; Eukaryota.
DR GeneTree; ENSGT00940000163113; -.
DR HOGENOM; CLU_012526_0_1_1; -.
DR InParanoid; A6NND4; -.
DR OMA; AITKITY; -.
DR OrthoDB; 1202809at2759; -.
DR PhylomeDB; A6NND4; -.
DR TreeFam; TF337194; -.
DR PathwayCommons; A6NND4; -.
DR Reactome; R-HSA-381753; Olfactory Signaling Pathway.
DR Reactome; R-HSA-9752946; Expression and translocation of olfactory receptors.
DR BioGRID-ORCS; 341152; 8 hits in 677 CRISPR screens.
DR ChiTaRS; OR2AT4; human.
DR GenomeRNAi; 341152; -.
DR Pharos; A6NND4; Tbio.
DR PRO; PR:A6NND4; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; A6NND4; protein.
DR Bgee; ENSG00000171561; Expressed in sural nerve and 1 other tissue.
DR ExpressionAtlas; A6NND4; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0004984; F:olfactory receptor activity; IDA:UniProtKB.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IMP:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000725; Olfact_rcpt.
DR Pfam; PF13853; 7tm_4; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00245; OLFACTORYR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Olfaction; Receptor; Reference proteome; Sensory transduction;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..320
FT /note="Olfactory receptor 2AT4"
FT /id="PRO_0000310866"
FT TOPO_DOM 1..31
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..102
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..145
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..200
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..276
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..320
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 102..184
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 320 AA; 35503 MW; 62E0FB03CCB8EADB CRC64;
MDATACNESV DGSPVFYLLG IPSLPETFFL PVFFIFLLFY LLILMGNALI LVAVVAEPSL
HKPMYFFLIN LSTLDILFTT TTVPKMLSLF LLGDRFLSFS SCLLQMYLFQ SFTCSEAFIL
VVMAYDRYVA ICHPLHYPVL MNPQTNATLA ASAWLTALLL PIPAVVRTSQ MAYNSIAYIY
HCFCDHLAVV QASCSDTTPQ TLMGFCIAMV VSFLPLLLVL LSYVHILASV LRISSLEGRA
KAFSTCSSHL LVVGTYYSSI AIAYVAYRAD LPLDFHIMGN VVYAILTPIL NPLIYTLRNR
DVKAAITKIM SQDPGCDRSI
