O2VC1_CONVC
ID O2VC1_CONVC Reviewed; 103 AA.
AC W4VSF6;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=O2 contryphan Vc1 {ECO:0000303|PubMed:24505301};
DE Short=O2_Vc1 {ECO:0000303|PubMed:24505301};
DE AltName: Full=Contryphan Vc1 {ECO:0000303|PubMed:28437072};
DE Short=Con-Vc1 {ECO:0000303|PubMed:28437072};
DE Flags: Precursor;
OS Conus victoriae (Queen Victoria cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX NCBI_TaxID=319920;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=24505301; DOI=10.1371/journal.pone.0087648;
RA Robinson S.D., Safavi-Hemami H., McIntosh L.D., Purcell A.W., Norton R.S.,
RA Papenfuss A.T.;
RT "Diversity of conotoxin gene superfamilies in the venomous snail, Conus
RT victoriae.";
RL PLoS ONE 9:E87648-E87648(2014).
RN [2]
RP PROTEIN SEQUENCE OF 68-98, IDENTIFICATION BY MASS SPECTROMETRY, SYNTHESIS
RP OF 68-98, STRUCTURE BY NMR OF 69-98, PYROGLUTAMATE FORMATION AT GLN-68, AND
RP DISULFIDE BOND.
RX PubMed=26774129; DOI=10.1016/j.str.2015.11.015;
RA Robinson S.D., Chhabra S., Belgi A., Chittoor B., Safavi-Hemami H.,
RA Robinson A.J., Papenfuss A.T., Purcell A.W., Norton R.S.;
RT "A naturally occurring peptide with an elementary single disulfide-directed
RT beta-hairpin fold.";
RL Structure 24:293-299(2016).
RN [3]
RP STRUCTURE BY NMR OF 68-89, AND DISULFIDE BOND.
RX PubMed=28437072; DOI=10.1021/acs.biochem.7b00120;
RA Chittoor B., Krishnarjuna B., Morales R.A.V., MacRaild C.A., Sadek M.,
RA Leung E.W.W., Robinson S.D., Pennington M.W., Norton R.S.;
RT "The single disulfide-directed beta-hairpin fold. dynamics, stability, and
RT engineering.";
RL Biochemistry 56:2455-2466(2017).
CC -!- FUNCTION: Unknown. Intracranial injection of the peptide into mice does
CC not produce toxic effects (PubMed:26774129). In addition, the pepide
CC does not produce any observable changes to normal or depolarization-
CC induced intracellular calcium levels in mouse dorsal root ganglion
CC cells (PubMed:26774129). {ECO:0000269|PubMed:26774129}.
CC -!- DOMAIN: Adopts the 'single disulfide-directed beta hairpin' (SDH) fold.
CC {ECO:0000305|PubMed:26774129, ECO:0000305|PubMed:28437072}.
CC -!- DOMAIN: The secondary structural features of this peptide are highly
CC resistant to thermal denaturation (PubMed:26774129). In addition, this
CC peptide can be truncated (90-103 DEL) without loss of ordered structure
CC (PubMed:28437072). {ECO:0000269|PubMed:26774129,
CC ECO:0000269|PubMed:28437072}.
CC -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC -!- PTM: Pyrrolidone carboxylic acid at position 1 has no significant
CC effect on the structure of contryphan-Vc1.
CC {ECO:0000269|PubMed:28437072}.
CC -!- SIMILARITY: Belongs to the O2 superfamily. {ECO:0000305}.
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DR EMBL; GAIH01000059; JAB84658.1; -; mRNA.
DR PDB; 2N24; NMR; -; A=68-98.
DR PDB; 5KKM; NMR; -; A=68-89.
DR PDBsum; 2N24; -.
DR PDBsum; 5KKM; -.
DR AlphaFoldDB; W4VSF6; -.
DR BMRB; W4VSF6; -.
DR SMR; W4VSF6; -.
PE 1: Evidence at protein level;
KW 3D-structure; Cleavage on pair of basic residues;
KW Direct protein sequencing; Disulfide bond; Pyrrolidone carboxylic acid;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..67
FT /evidence="ECO:0000305|PubMed:26774129"
FT /id="PRO_0000445679"
FT PEPTIDE 68..98
FT /note="O2 contryphan Vc1"
FT /evidence="ECO:0000269|PubMed:26774129"
FT /id="PRO_5004852236"
FT PROPEP 99..103
FT /evidence="ECO:0000305|PubMed:26774129"
FT /id="PRO_0000445680"
FT REGION 25..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 68
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:26774129"
FT DISULFID 70..83
FT /evidence="ECO:0000269|PubMed:28437072,
FT ECO:0007744|PDB:2N24, ECO:0007744|PDB:5KKM"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:2N24"
FT TURN 78..80
FT /evidence="ECO:0007829|PDB:2N24"
FT STRAND 81..85
FT /evidence="ECO:0007829|PDB:2N24"
FT HELIX 87..90
FT /evidence="ECO:0007829|PDB:2N24"
FT TURN 93..96
FT /evidence="ECO:0007829|PDB:2N24"
SQ SEQUENCE 103 AA; 11629 MW; A7FC1B4A010517C6 CRC64;
MGKLTILFLV AAALLSTQVM VQGDGAHERT EAEEPQHHGA KRQDGTGGYP VDDVDMMQRI
FRTPLKRQWC QPGYAYNPVL GICTITLSRI EHPGNYDYRR GRQ
