ID   O3_VACCW                Reviewed;          35 AA.
AC   P0CK21;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   12-AUG-2020, entry version 17.
DE   RecName: Full=Protein O3;
GN   OrderedLocusNames=VACWR069.5; ORFNames=O3L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   CHARACTERIZATION, AND SUBCELLULAR LOCATION.
RX   PubMed=19812151; DOI=10.1128/jvi.01744-09;
RA   Satheshkumar P.S., Moss B.;
RT   "Characterization of a newly identified 35-amino-acid component of the
RT   vaccinia virus entry/fusion complex conserved in all chordopoxviruses.";
RL   J. Virol. 83:12822-12832(2009).
CC   -!- FUNCTION: Late protein which associates with the entry/fusion complex
CC       (EFC). Needed for fusion and penetration of the virus cores into host
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}. Note=Localizes in cytoplasmic virus
CC       factories. Component of the membrane of the mature virion.
CC       {ECO:0000269|PubMed:19812151}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae O3 family. {ECO:0000305}.
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DR   EMBL; AY243312; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Late protein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Viral envelope protein;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..35
FT                   /note="Protein O3"
FT                   /id="PRO_0000411966"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..35
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   35 AA;  4226 MW;  BFF68C35E436CDA0 CRC64;
     MLVVIMFFIA FAFCSWLSYS YLRPYISTKE LNKSR