OAF3_YEAST
ID OAF3_YEAST Reviewed; 863 AA.
AC P36023; D6VXC5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Oleate activated transcription factor 3;
GN Name=OAF3; OrderedLocusNames=YKR064W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [6]
RP DOMAIN, AND PREDICTION OF FUNCTION.
RX PubMed=16959962; DOI=10.1128/mmbr.00015-06;
RA MacPherson S., Larochelle M., Turcotte B.;
RT "A fungal family of transcriptional regulators: the zinc cluster
RT proteins.";
RL Microbiol. Mol. Biol. Rev. 70:583-604(2006).
RN [7]
RP FUNCTION.
RX PubMed=17551510; DOI=10.1038/msb4100157;
RA Smith J.J., Ramsey S.A., Marelli M., Marzolf B., Hwang D., Saleem R.A.,
RA Rachubinski R.A., Aitchison J.D.;
RT "Transcriptional responses to fatty acid are coordinated by combinatorial
RT control.";
RL Mol. Syst. Biol. 3:115-115(2007).
RN [8]
RP FUNCTION.
RX PubMed=18621837; DOI=10.1529/biophysj.108.134064;
RA Ratushny A.V., Ramsey S.A., Roda O., Wan Y., Smith J.J., Aitchison J.D.;
RT "Control of transcriptional variability by overlapping feed-forward
RT regulatory motifs.";
RL Biophys. J. 95:3715-3723(2008).
CC -!- FUNCTION: Transcriptional inhibitor with a significantly increased
CC number of target genes in response to oleate.
CC {ECO:0000269|PubMed:17551510, ECO:0000269|PubMed:18621837}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Mitochondrion.
CC -!- MISCELLANEOUS: Present with 149 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the OAF3 family. {ECO:0000305}.
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DR EMBL; Z28289; CAA82143.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09215.1; -; Genomic_DNA.
DR PIR; S38140; S38140.
DR RefSeq; NP_012990.1; NM_001179854.1.
DR AlphaFoldDB; P36023; -.
DR BioGRID; 34195; 71.
DR DIP; DIP-2792N; -.
DR IntAct; P36023; 4.
DR MINT; P36023; -.
DR STRING; 4932.YKR064W; -.
DR MaxQB; P36023; -.
DR PaxDb; P36023; -.
DR PRIDE; P36023; -.
DR EnsemblFungi; YKR064W_mRNA; YKR064W; YKR064W.
DR GeneID; 853938; -.
DR KEGG; sce:YKR064W; -.
DR SGD; S000001772; OAF3.
DR VEuPathDB; FungiDB:YKR064W; -.
DR eggNOG; ENOG502QQCV; Eukaryota.
DR GeneTree; ENSGT00940000176581; -.
DR HOGENOM; CLU_018684_0_0_1; -.
DR InParanoid; P36023; -.
DR OMA; WCAPEDG; -.
DR BioCyc; YEAST:G3O-32032-MON; -.
DR PRO; PR:P36023; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36023; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Metal-binding; Mitochondrion; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation;
KW Zinc.
FT CHAIN 1..863
FT /note="Oleate activated transcription factor 3"
FT /id="PRO_0000115002"
FT DNA_BIND 18..47
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 52..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..69
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..99
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 863 AA; 101825 MW; B140465B405FAF84 CRC64;
MGYDSQVRTK KRHRITVVCT NCKKRKSKCD RTKPCGTCVR LGDVDSCVYL TDSSGQPESS
PSLNDADPLR KQSTPAERIS PGFIKKRRSS QTRQDEDHWQ RVRELENQSS LYYLPIHEET
PFFIDLIPNG FYLETKRSAD NLFGLFTDRA IENRDPYLQA MVTFRSIAIK KMMDKLGSNG
NNVKNGSLPK SFEALSTFDA DDERHISDDV VGKGNNFRMH QTIHKSLFNK FAQYRENNAK
KFSSETILAK DYLPPLKILE SEVLALFEEK IYNMIPIFDM KVLRHEITIF YQNIVEKGNP
ISIKHYDHMV FCIILLIIKI CRLSVQFSKL TPYIYPVLQE IDTSKFLALV KHYLFETKVL
RKCNLLQLQC LILLRFLHWC APEDGDGPET QYCQILMGTI ISSCKEMGIN WYCFSHPEKY
SFKINRHTRP SYDIMKPSDY ISVFRKIWSY VLFWDRKMCF ISGEECQIGK TLQCHFKEEA
DTPTWYIRML TLDNLMKKIN DTLNDDPGKV DLNLLHRLIN DLKRNFHILK SLSKNEKETM
RHFDFEMEWI IDLFSLSLLH GEMIFYEYDC NITKFYKSFQ DLWDMVIHIS EKCYNYFFNS
DALEVDSLTK FYTNRIVEIV ANKVLVIVPA FILRGDRFKT IQYADKKKMI EFLYGVSSVY
FNEFGFEYYR CFRKMFTAKI AYKILNRSCE KDAWRIILKF LLNELKLEDN GDSYIDYNDM
RLNDICPIIL EFQETVQKYD GYRPDILSIW NNEFYPIGKY NDDMTGFKFQ MRIKEMQEFL
DMEKYSDRFN IFSSFYDHAS SQLAKHTEVD TNISITNEQV AEIPQKELLQ QPLAPALPVN
DLIVSEFDVI EDIFDPVDFV SFF
