OAF_DROME
ID OAF_DROME Reviewed; 487 AA.
AC Q9NLA6; Q24556; Q86CS3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Out at first protein;
DE Contains:
DE RecName: Full=Out at first short protein;
GN Name=oaf; ORFNames=CG9884;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=7768442; DOI=10.1093/genetics/139.3.1331;
RA Bergstrom D.E., Merli C.A., Cygan J.A., Shelby R., Blackman R.K.;
RT "Regulatory autonomy and molecular characterization of the Drosophila out
RT at first gene.";
RL Genetics 139:1331-1346(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-89, AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R;
RX PubMed=8675012; DOI=10.1101/gad.10.10.1260;
RA Merli C., Bergstrom D.E., Cygan J.A., Blackman R.K.;
RT "Promoter specificity mediates the independent regulation of neighboring
RT genes.";
RL Genes Dev. 10:1260-1270(1996).
CC -!- FUNCTION: Vital for proper neuronal development and hatching.
CC -!- TISSUE SPECIFICITY: Embryonic expression is in small clusters of cells
CC along posterior margin of most segments, brain and segmentally
CC repeating pattern along midline of nerve cord. Expressed in embryonic,
CC larval and adult gonads of both sexes, and larval imaginal disks.
CC {ECO:0000269|PubMed:8675012}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development with highest levels in pupae and adults.
CC -!- MISCELLANEOUS: Readthrough of the terminator UGA may occur between the
CC codons for 332-Glu and 334-Arg.
CC -!- SIMILARITY: Belongs to the OAF family. {ECO:0000305}.
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DR EMBL; L31349; AAC37219.2; -; mRNA.
DR EMBL; AE014134; AAF51246.1; -; Genomic_DNA.
DR EMBL; AE014134; AAO41163.2; -; Genomic_DNA.
DR EMBL; U63852; AAC47551.1; -; Genomic_DNA.
DR PIR; S55016; S55016.
DR RefSeq; NP_477040.1; NM_057692.2.
DR RefSeq; NP_722815.1; NM_164489.2.
DR RefSeq; NP_722816.1; NM_164490.1.
DR RefSeq; NP_787963.2; NM_175949.1.
DR BioGRID; 59662; 1.
DR STRING; 7227.FBpp0077456; -.
DR PaxDb; Q9NLA6; -.
DR PRIDE; Q9NLA6; -.
DR DNASU; 33435; -.
DR EnsemblMetazoa; FBtr0077780; FBpp0077459; FBgn0011818.
DR GeneID; 33435; -.
DR KEGG; dme:Dmel_CG9884; -.
DR CTD; 220323; -.
DR FlyBase; FBgn0011818; oaf.
DR VEuPathDB; VectorBase:FBgn0011818; -.
DR eggNOG; ENOG502QWDA; Eukaryota.
DR GeneTree; ENSGT00390000012008; -.
DR InParanoid; Q9NLA6; -.
DR PhylomeDB; Q9NLA6; -.
DR BioGRID-ORCS; 33435; 0 hits in 1 CRISPR screen.
DR ChiTaRS; oaf; fly.
DR GenomeRNAi; 33435; -.
DR PRO; PR:Q9NLA6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011818; Expressed in cleaving embryo and 55 other tissues.
DR ExpressionAtlas; Q9NLA6; baseline and differential.
DR Genevisible; Q9NLA6; DM.
DR InterPro; IPR026315; Oaf.
DR PANTHER; PTHR13423; PTHR13423; 1.
DR Pfam; PF14941; OAF; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Reference proteome.
FT CHAIN 1..487
FT /note="Out at first protein"
FT /id="PRO_0000021871"
FT CHAIN 1..332
FT /note="Out at first short protein"
FT /id="PRO_0000021872"
FT REGION 372..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 423..487
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 391
FT /note="A -> G (in Ref. 1; AAC37219)"
FT /evidence="ECO:0000305"
FT CONFLICT 400
FT /note="R -> H (in Ref. 1; AAC37219)"
FT /evidence="ECO:0000305"
FT CONFLICT 413
FT /note="A -> T (in Ref. 1; AAC37219)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 487 AA; 53730 MW; F0D7A117BE358B3F CRC64;
MILKEEHPHQ SIETAANAAR QAQVRWRMAH LKALSRTRTP AHGNCCGRVV SKNHFFKHSR
AFLWFLLCNL VMNADAFAHS QLLINVQNQG GEVIQESITS NIGEDLITLE FQKTDGTLIT
QVIDFRNEVQ ILKALVLGEE ERGQSQYQVM CFATKFNKGD FISSAAMAKL RQKNPHTIRT
PEEDKGRETF TMSSWVQLNR SLPITRHLQG LCAEAMDATY VRDVDLKAWA ELPGSSISSL
EAATEKFPDT LSTRCNEVSS LWAPCLCNLE TCIGWYPCGL KYCKGKGVAG ADSSGAQQQA
QPTNYRCGIK TCRKCTQFTY YVRQKQQCLW DEXRRGELQL MQMRCARRRN GSEFGDDASA
TCPGGETRAA TTTATITGGG AGGSGKDTTA ATTTTTNKLR QLLLLVQQQM PFALWSFPVH
HISQSHHQSQ SQHKPSRQQK QHQHHSQVAP TSHHQSSSST PPTPSTSSSP PSSSSSSSSS
AMAAIVA
