OATA_STAAN
ID OATA_STAAN Reviewed; 603 AA.
AC Q7A3D6;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=O-acetyltransferase OatA;
DE EC=2.3.1.- {ECO:0000250|UniProtKB:Q2FV54};
GN Name=oatA; OrderedLocusNames=SA2354;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N315;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=N315;
RA Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT aureus strain N315.";
RL Submitted (OCT-2007) to UniProtKB.
CC -!- FUNCTION: Responsible for O-acetylation at the C(6)-hydroxyl group of
CC N-acetylmuramyl residues, forming the corresponding N,6-O-
CC diacetylmuramic acid of the peptidoglycan. O-acetylation of the
CC peptidoglycan is the major determinant for lysozyme resistance.
CC {ECO:0000250|UniProtKB:Q2FV54}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the acyltransferase 3 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000018; BAB43658.1; -; Genomic_DNA.
DR PIR; H90061; H90061.
DR RefSeq; WP_000379821.1; NC_002745.2.
DR AlphaFoldDB; Q7A3D6; -.
DR SMR; Q7A3D6; -.
DR EnsemblBacteria; BAB43658; BAB43658; BAB43658.
DR KEGG; sau:SA2354; -.
DR HOGENOM; CLU_005679_11_2_9; -.
DR OMA; NRWLTNP; -.
DR Proteomes; UP000000751; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR002656; Acyl_transf_3_dom.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR Pfam; PF01757; Acyl_transf_3; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Cell membrane; Membrane; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..603
FT /note="O-acetyltransferase OatA"
FT /id="PRO_0000208087"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 453
FT /evidence="ECO:0000250|UniProtKB:Q2FV54"
FT ACT_SITE 575
FT /evidence="ECO:0000250|UniProtKB:Q2FV54"
FT ACT_SITE 578
FT /evidence="ECO:0000250|UniProtKB:Q2FV54"
SQ SEQUENCE 603 AA; 69099 MW; B9050E0DE20B8DF1 CRC64;
MDTKDFKRLE KMYSPRYLPG LDGLRAFAVI GIIIYHLNAQ WLSGGFLGVD TFFVISGYLI
TSLLISEYYR TQKIDLLEFW KRRLKRLIPA VLFLICVVLT FTLIFKPELI IQMKRDAIAA
IFYVSNWWYI SQNVDYFNQF AIEPLKHLWS LAIEEQFYLL FPLVITFLLH RFKPRNIIQT
LFIVSLISLG LMIVIHFITG DNSRVYFGTD TRLQTLLLGC ILAFIWPPFA LKKDISKKIV
VSLDIIGISG FAVLMTLFFI VGDQDQWIYN GGFYIISFAT LFIIAIAVHP SSLFAKFLSM
KPLLIIGKRS YSLYLWHYPI IVFVNSYYVQ GQIPVYVYII EILLTALMAE ISYRFIETPI
RKKGFKAFAF LPKKKGQFAR TVLVILLLVP SIVVLSGQFD ALGKQHEAEK KEKKTEFKTT
KKKVVKKDKQ EDKQTANSKE DIKKSSPLLI GDSVMVDIGN VFTKKIPNAQ IDGKVGRQLV
DATPIVKSQY KDYAKKGQKV VVELGTNGAF TKDQLNELLD SFGKADIYLV SIRVPRDYEG
RINKLIYEAA EKRSNVHLVD WYKASAGHPE YFAYDGIHLE YAGSKALTDL IVKTMETHAT
NKK