OB28A_DROME
ID OB28A_DROME Reviewed; 143 AA.
AC P54195; Q4V3T8; Q9VM03;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=General odorant-binding protein 28a;
DE AltName: Full=Odorant-binding protein 28a;
DE AltName: Full=Pheromone-binding protein-related protein 5;
DE Short=PBPRP-5;
DE Flags: Precursor;
GN Name=Obp28a; Synonyms=Pbprp5; ORFNames=CG6641;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Antenna;
RX PubMed=7545907; DOI=10.1016/0896-6273(94)90150-3;
RA Pikielny C.W., Hasan G., Rouyer F., Rosbash M.;
RT "Members of a family of Drosophila putative odorant-binding proteins are
RT expressed in different subsets of olfactory hairs.";
RL Neuron 12:35-49(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Note=Secreted in the
CC lumen of olfactory hairs. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in antenna, mostly on the medial and
CC posterior surface of the third antennal segment.
CC {ECO:0000269|PubMed:7545907}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U05985; AAC46478.1; -; mRNA.
DR EMBL; AE014134; AAF52525.1; -; Genomic_DNA.
DR EMBL; BT023268; AAY55684.1; -; mRNA.
DR RefSeq; NP_523505.1; NM_078781.3.
DR PDB; 6QQ4; X-ray; 2.00 A; A/B=23-143.
DR PDBsum; 6QQ4; -.
DR AlphaFoldDB; P54195; -.
DR SMR; P54195; -.
DR STRING; 7227.FBpp0079083; -.
DR PaxDb; P54195; -.
DR DNASU; 34031; -.
DR EnsemblMetazoa; FBtr0079455; FBpp0079083; FBgn0011283.
DR GeneID; 34031; -.
DR KEGG; dme:Dmel_CG6641; -.
DR CTD; 34031; -.
DR FlyBase; FBgn0011283; Obp28a.
DR VEuPathDB; VectorBase:FBgn0011283; -.
DR eggNOG; ENOG502T2AN; Eukaryota.
DR GeneTree; ENSGT00520000058998; -.
DR HOGENOM; CLU_148261_0_0_1; -.
DR InParanoid; P54195; -.
DR OMA; YAGKCLR; -.
DR OrthoDB; 1320220at2759; -.
DR PhylomeDB; P54195; -.
DR BioGRID-ORCS; 34031; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34031; -.
DR PRO; PR:P54195; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011283; Expressed in antennal segment and 29 other tissues.
DR Genevisible; P54195; DM.
DR GO; GO:0005576; C:extracellular region; ISM:FlyBase.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0005550; F:pheromone binding; ISS:FlyBase.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; ISS:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; IBA:GO_Central.
DR Gene3D; 1.10.238.20; -; 1.
DR InterPro; IPR006170; PBP/GOBP.
DR InterPro; IPR036728; PBP_GOBP_sf.
DR Pfam; PF01395; PBP_GOBP; 1.
DR SMART; SM00708; PhBP; 1.
DR SUPFAM; SSF47565; SSF47565; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..143
FT /note="General odorant-binding protein 28a"
FT /id="PRO_0000012590"
FT DISULFID 38..69
FT /evidence="ECO:0000250"
FT DISULFID 65..123
FT /evidence="ECO:0000250"
FT DISULFID 113..132
FT /evidence="ECO:0000250"
FT CONFLICT 108
FT /note="E -> D (in Ref. 1; AAC46478)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="D -> E (in Ref. 1; AAC46478)"
FT /evidence="ECO:0000305"
FT HELIX 24..38
FT /evidence="ECO:0007829|PDB:6QQ4"
FT HELIX 39..42
FT /evidence="ECO:0007829|PDB:6QQ4"
FT HELIX 46..53
FT /evidence="ECO:0007829|PDB:6QQ4"
FT HELIX 61..74
FT /evidence="ECO:0007829|PDB:6QQ4"
FT STRAND 75..77
FT /evidence="ECO:0007829|PDB:6QQ4"
FT STRAND 81..83
FT /evidence="ECO:0007829|PDB:6QQ4"
FT HELIX 88..96
FT /evidence="ECO:0007829|PDB:6QQ4"
FT HELIX 100..113
FT /evidence="ECO:0007829|PDB:6QQ4"
FT HELIX 122..139
FT /evidence="ECO:0007829|PDB:6QQ4"
SQ SEQUENCE 143 AA; 15152 MW; AFF9CBC367E750B3 CRC64;
MQSTPIILVA IVLLGAALVR AFDEKEALAK LMESAESCMP EVGATDADLQ EMVKKQPAST
YAGKCLRACV MKNIGILDAN GKLDTEAGHE KAKQYTGNDP AKLKIALEIG DTCAAITVPD
DHCEAAEAYG TCFRGEAKKH GLL
