OBE2_ARATH
ID OBE2_ARATH Reviewed; 574 AA.
AC Q9LUB7; B9DH01; Q0WU99; Q570W2;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein OBERON 2 {ECO:0000303|PubMed:18403411};
DE AltName: Full=Potyvirus VPg-interacting protein 1 {ECO:0000303|PubMed:14963126};
GN Name=OBE2 {ECO:0000303|PubMed:18403411};
GN Synonyms=PVIP1 {ECO:0000303|PubMed:14963126};
GN OrderedLocusNames=At5g48160 {ECO:0000312|Araport:AT5G48160};
GN ORFNames=MIF21.5 {ECO:0000312|EMBL:BAA96996.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH POTYVIRUS VPG PROTEIN.
RX PubMed=14963126; DOI=10.1128/jvi.78.5.2301-2309.2004;
RA Dunoyer P., Thomas C., Harrison S., Revers F., Maule A.;
RT "A cysteine-rich plant protein potentiates Potyvirus movement through an
RT interaction with the virus genome-linked protein VPg.";
RL J. Virol. 78:2301-2309(2004).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DIMERIZATION.
RX PubMed=18403411; DOI=10.1242/dev.014993;
RA Saiga S., Furumizu C., Yokoyama R., Kurata T., Sato S., Kato T., Tabata S.,
RA Suzuki M., Komeda Y.;
RT "The Arabidopsis OBERON1 and OBERON2 genes encode plant homeodomain finger
RT proteins and are required for apical meristem maintenance.";
RL Development 135:1751-1759(2008).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INDUCTION BY AUXIN, SUBUNIT, AND INTERACTION WITH OBE1; OBE3 AND OBE4.
RX PubMed=19392692; DOI=10.1111/j.1365-313x.2009.03874.x;
RA Thomas C.L., Schmidt D., Bayer E.M., Dreos R., Maule A.J.;
RT "Arabidopsis plant homeodomain finger proteins operate downstream of auxin
RT accumulation in specifying the vasculature and primary root meristem.";
RL Plant J. 59:426-436(2009).
RN [8]
RP INTERACTION WITH OBE1; OBE3 AND OBE4.
RX PubMed=22378640; DOI=10.1242/dev.074492;
RA Saiga S., Moeller B., Watanabe-Taneda A., Abe M., Weijers D., Komeda Y.;
RT "Control of embryonic meristem initiation in Arabidopsis by PHD-finger
RT protein complexes.";
RL Development 139:1391-1398(2012).
CC -!- FUNCTION: Probable transcription factor that acts together with OBE1
CC for the maintenance and/or establishment of both the shoot and root
CC meristems, probably by controlling the expression of the meristem genes
CC such as WUS, PLT1 and PLT2 and of genes required for auxin responses.
CC Promotes cell meristematic activity via the WUSCHEL-CLAVATA pathway.
CC Involved in the development of the basal pole and in auxin-mediated
CC root and vascular development in the embryo. Confers sensitivity to
CC turnip mosaic virus (TuMV) probably by promoting viral movement and
CC multiplication via interaction with TuMV VPg.
CC {ECO:0000269|PubMed:18403411, ECO:0000269|PubMed:19392692}.
CC -!- SUBUNIT: Self-interacts (PubMed:18403411, PubMed:19392692,
CC PubMed:22378640). Interacts with OBE1, OBE3 and OBE4 (PubMed:19392692,
CC PubMed:22378640). Binds to VPg of pea seed borne mosaic virus (PSbMV),
CC turnip mosaic virus (TuMV) and lettuce mosaic virus (LMV), but not with
CC VPg of tobacco etch virus (TEV), cowpea mosaic virus (CPMV), tomato
CC black ring virus (TBRV) and grapevine fan leaf virus (GFLV)
CC (PubMed:14963126). {ECO:0000269|PubMed:14963126,
CC ECO:0000269|PubMed:18403411, ECO:0000269|PubMed:19392692,
CC ECO:0000269|PubMed:22378640}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18403411}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, seedlings, stems, leaves,
CC flowers and siliques, especially in the vasculature.
CC {ECO:0000269|PubMed:18403411, ECO:0000269|PubMed:19392692}.
CC -!- DEVELOPMENTAL STAGE: First observed in the embryo proper at the four-
CC cell stage. Later expressed throughout the embryo from the eight-cell
CC to the bent-cotyledon stages. Until the torpedo stage of development,
CC mostly concentrated at the root pole. Hardly detected in the suspensor.
CC Present in seedling roots. In mature and fully differentiated young
CC roots, restricted to root tips. {ECO:0000269|PubMed:18403411,
CC ECO:0000269|PubMed:19392692}.
CC -!- INDUCTION: By auxin in the root elongation zone.
CC {ECO:0000269|PubMed:19392692}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. When associated with OBE1
CC disruption, plants exhibit premature termination of the shoot meristem
CC and impaired root apical meristem establishment, leading to a
CC diminutive phenotype characterized by an absence of roots and defective
CC development of the vasculature. {ECO:0000269|PubMed:18403411,
CC ECO:0000269|PubMed:19392692}.
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DR EMBL; AB023039; BAA96996.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95626.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95627.1; -; Genomic_DNA.
DR EMBL; AK317346; BAH20018.1; -; mRNA.
DR EMBL; AK317672; BAH20332.1; -; mRNA.
DR EMBL; AK220595; BAD94916.1; -; mRNA.
DR EMBL; AK227275; BAE99299.1; -; mRNA.
DR RefSeq; NP_001032031.1; NM_001036954.1.
DR RefSeq; NP_199627.1; NM_124190.4.
DR AlphaFoldDB; Q9LUB7; -.
DR BioGRID; 20115; 5.
DR STRING; 3702.AT5G48160.2; -.
DR iPTMnet; Q9LUB7; -.
DR PaxDb; Q9LUB7; -.
DR PRIDE; Q9LUB7; -.
DR ProteomicsDB; 250953; -.
DR EnsemblPlants; AT5G48160.1; AT5G48160.1; AT5G48160.
DR EnsemblPlants; AT5G48160.2; AT5G48160.2; AT5G48160.
DR GeneID; 834868; -.
DR Gramene; AT5G48160.1; AT5G48160.1; AT5G48160.
DR Gramene; AT5G48160.2; AT5G48160.2; AT5G48160.
DR KEGG; ath:AT5G48160; -.
DR Araport; AT5G48160; -.
DR TAIR; locus:2164713; AT5G48160.
DR eggNOG; ENOG502QSQF; Eukaryota.
DR HOGENOM; CLU_006737_2_0_1; -.
DR InParanoid; Q9LUB7; -.
DR OMA; SRETWPA; -.
DR OrthoDB; 428651at2759; -.
DR PhylomeDB; Q9LUB7; -.
DR PRO; PR:Q9LUB7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LUB7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IGI:TAIR.
DR GO; GO:0010078; P:maintenance of root meristem identity; IGI:TAIR.
DR GO; GO:0010492; P:maintenance of shoot apical meristem identity; IGI:TAIR.
DR GO; GO:0080022; P:primary root development; IGI:TAIR.
DR GO; GO:0010468; P:regulation of gene expression; IGI:TAIR.
DR GO; GO:0010071; P:root meristem specification; IGI:TAIR.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IMP:UniProtKB.
DR InterPro; IPR004082; OBERON.
DR InterPro; IPR032535; Oberon_cc.
DR InterPro; IPR032881; Oberon_PHD.
DR InterPro; IPR001965; Znf_PHD.
DR PANTHER; PTHR21736; PTHR21736; 1.
DR Pfam; PF16312; Oberon_cc; 1.
DR Pfam; PF07227; PHD_Oberon; 1.
DR PIRSF; PIRSF025218; DUF1423_pln; 1.
DR PRINTS; PR01544; ARATH130DUF.
DR SMART; SM00249; PHD; 1.
DR PROSITE; PS01359; ZF_PHD_1; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Developmental protein; Host-virus interaction; Metal-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..574
FT /note="Protein OBERON 2"
FT /id="PRO_0000399747"
FT ZN_FING 226..291
FT /note="PHD-type"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 416..524
FT /evidence="ECO:0000255"
FT COMPBIAS 1..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 136
FT /note="D -> G (in Ref. 4; BAE99299)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="L -> F (in Ref. 3; BAH20018)"
FT /evidence="ECO:0000305"
FT CONFLICT 493
FT /note="E -> G (in Ref. 4; BAE99299)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 574 AA; 65084 MW; A12D0462A0439442 CRC64;
MGTSSGSNHP HQMLPPRQQQ RSGGGLETAL SLVSSDQEPR RESPAESASS QETWPLGDTV
AGKKSMSQKT EPDSMEQTVN VMHHVSNADK VSVRDIARER VELVAERMHR LPDEFLDELK
NGLKSILEGN VAQSVDEFMF LQKVVQSRTD LSSVTLVRAH RVQLEILVAI NTGIQAFLHP
NISLSQPSLI EIFVYKRCRN IACQNQLPAD DCYCDICTNR KGFCNLCMCT ICNKFDFSVN
TCRWIGCDLC SHWTHTDCAI RDGQITTGSS AKNNTSGPGE IVFKCRACNR TSELLGWVKD
VFQHCAPNWD RESLMKELDF VSRIFRGSED QRGRKLFWKC EELIDKIKGG LAEATAAKLI
LMFFQEIESD SAKSFENGEG GRLMAPQDAC NRIAEVVQET LRKMEIVAEE KMRMFKKARM
ALETCDRELE DKAKEVSELK AERQKKKLQI DELERIVRLK QAEADMFQLK ANEAKREADR
LQRIVLAKMD KSEEEYASNY LKQRLSEAEA EKQYLFEKIK LQENSRVASQ SSGGGGDPSQ
VMMYSKIRDL LQGYNLSPKV DPQLNERNPF RSNP
