OBE4_ARATH
ID OBE4_ARATH Reviewed; 1162 AA.
AC Q84TI3; Q9LY65;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Protein OBERON 4 {ECO:0000305};
DE AltName: Full=Protein TITANIA 1 {ECO:0000303|PubMed:22378640};
GN Name=OBE4 {ECO:0000305};
GN OrderedLocusNames=At3g63500 {ECO:0000312|Araport:AT3G63500};
GN ORFNames=MAA21.130 {ECO:0000312|EMBL:CAB87803.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH OBE1 AND OBE2.
RX PubMed=19392692; DOI=10.1111/j.1365-313x.2009.03874.x;
RA Thomas C.L., Schmidt D., Bayer E.M., Dreos R., Maule A.J.;
RT "Arabidopsis plant homeodomain finger proteins operate downstream of auxin
RT accumulation in specifying the vasculature and primary root meristem.";
RL Plant J. 59:426-436(2009).
RN [6]
RP FUNCTION, INTERACTION WITH OBE1; OBE2 AND OBE3, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=22378640; DOI=10.1242/dev.074492;
RA Saiga S., Moeller B., Watanabe-Taneda A., Abe M., Weijers D., Komeda Y.;
RT "Control of embryonic meristem initiation in Arabidopsis by PHD-finger
RT protein complexes.";
RL Development 139:1391-1398(2012).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27196372; DOI=10.1371/journal.pone.0155657;
RA Lin T.F., Saiga S., Abe M., Laux T.;
RT "OBE3 and WUS interaction in shoot meristem stem cell regulation.";
RL PLoS ONE 11:E0155657-E0155657(2016).
CC -!- FUNCTION: Probable transcription factor that functions redundantly with
CC OBE3 in specification of the hypophysis and establishment of the
CC embryonic root (PubMed:22378640). Involved in the activation of
CC ARF5/MP-dependent gene expression during embryonic root meristem
CC initiation (PubMed:22378640). Involved in shoot meristem homeostasis
CC (PubMed:27196372). {ECO:0000269|PubMed:22378640,
CC ECO:0000269|PubMed:27196372}.
CC -!- SUBUNIT: Self-interacts (PubMed:22378640). Interacts with OBE1 and OBE2
CC (PubMed:19392692, PubMed:22378640). Interacts with OBE3
CC (PubMed:22378640). {ECO:0000269|PubMed:19392692,
CC ECO:0000269|PubMed:22378640}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9S736}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q84TI3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q84TI3-2; Sequence=VSP_041312, VSP_041313;
CC -!- DEVELOPMENTAL STAGE: Expressed in the two-cell stage embryo
CC (PubMed:22378640). At early globular embryo stage, expressed both in
CC the basal region of embryo proper and suspensor cells
CC (PubMed:22378640). At heart and torpedo embryo stages expressed in the
CC basal region apical regions of the embryo proper (PubMed:22378640).
CC {ECO:0000269|PubMed:22378640}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but the double mutants tta1 and tta2 exhibit a rootless
CC phenotype and seedling lethality (PubMed:22378640). No visible
CC phenotype under normal growth conditions, but the double mutants obe3-2
CC and obe4-2 exhibit broad growth defects and developmental arrest of
CC seedlings (PubMed:27196372). {ECO:0000269|PubMed:22378640,
CC ECO:0000269|PubMed:27196372}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB87803.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL163818; CAB87803.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80495.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64027.1; -; Genomic_DNA.
DR EMBL; BT005768; AAO64172.1; -; mRNA.
DR EMBL; AK228553; BAF00474.1; -; mRNA.
DR PIR; T49191; T49191.
DR RefSeq; NP_001326078.1; NM_001340219.1. [Q84TI3-1]
DR RefSeq; NP_191909.1; NM_116215.4. [Q84TI3-1]
DR AlphaFoldDB; Q84TI3; -.
DR SMR; Q84TI3; -.
DR BioGRID; 10839; 6.
DR STRING; 3702.AT3G63500.2; -.
DR iPTMnet; Q84TI3; -.
DR PaxDb; Q84TI3; -.
DR PRIDE; Q84TI3; -.
DR ProteomicsDB; 250854; -. [Q84TI3-1]
DR EnsemblPlants; AT3G63500.2; AT3G63500.2; AT3G63500. [Q84TI3-1]
DR EnsemblPlants; AT3G63500.3; AT3G63500.3; AT3G63500. [Q84TI3-1]
DR GeneID; 825525; -.
DR Gramene; AT3G63500.2; AT3G63500.2; AT3G63500. [Q84TI3-1]
DR Gramene; AT3G63500.3; AT3G63500.3; AT3G63500. [Q84TI3-1]
DR KEGG; ath:AT3G63500; -.
DR Araport; AT3G63500; -.
DR TAIR; locus:2087403; AT3G63500.
DR eggNOG; ENOG502QPTA; Eukaryota.
DR HOGENOM; CLU_006737_1_0_1; -.
DR InParanoid; Q84TI3; -.
DR OrthoDB; 183360at2759; -.
DR PRO; PR:Q84TI3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q84TI3; baseline and differential.
DR Genevisible; Q84TI3; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0001708; P:cell fate specification; IGI:TAIR.
DR GO; GO:0090421; P:embryonic meristem initiation; IGI:TAIR.
DR GO; GO:0009880; P:embryonic pattern specification; IGI:TAIR.
DR InterPro; IPR004082; OBERON.
DR InterPro; IPR032535; Oberon_cc.
DR InterPro; IPR032881; Oberon_PHD.
DR PANTHER; PTHR21736; PTHR21736; 1.
DR Pfam; PF16312; Oberon_cc; 1.
DR Pfam; PF07227; PHD_Oberon; 1.
DR PRINTS; PR01544; ARATH130DUF.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..1162
FT /note="Protein OBERON 4"
FT /id="PRO_0000399749"
FT ZN_FING 835..899
FT /note="PHD-type"
FT REGION 1..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 251..307
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 441..485
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1065..1161
FT /evidence="ECO:0000255"
FT COMPBIAS 1..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..102
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..137
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..183
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 253..267
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..297
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 441..460
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..187
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.4"
FT /id="VSP_041312"
FT VAR_SEQ 188..255
FT /note="KSEEEVQGKSSTTSSEMEEGELEPEPQPETASGLAHQTKHDCKLPSCSADDH
FT KNARIDRSFQEIGKSA -> MIRMLTENLPTRTVRPLPPTGASSRATTVTAGKMLPASP
FT GLFPVGDPIVTWIIIGQMPGTIDLRAPVS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.4"
FT /id="VSP_041313"
SQ SEQUENCE 1162 AA; 130732 MW; 5B65878EC17FCC7D CRC64;
MKRLRSSDDL DFCNDKNVDG EPPNSDRPAS SSHRGFFSGN NRDRGEDAAG FSRAFSRRRS
NRDLDNHRPD ARYHRSESAC FSRRAFPKGF RSERERPNRD ASVSSWRRFG GPGNDFGVDD
RDRRLRDAER DRSLKSPSWS RDSPNELSKF KPLDSRNSRS RSKSLASPTW SKDSGSEQSK
SVGNVVKKSE EEVQGKSSTT SSEMEEGELE PEPQPETASG LAHQTKHDCK LPSCSADDHK
NARIDRSFQE IGKSAQLDAN TESNRELSHV GGNREMETTD SMTDKKSVED AENVPEHATE
SMHVSQNNVN DTSTALAIEH DHRDGTITAS ANKITDTVDE KGDKDEDYKE NLHGVKLEET
LYPDVPERLE ELKEVKGNDG DANKAEVEGP ECVEENALAN RTPAEYISSV SDSSVHKCKD
KGKNSDVPLT HLVGNALFSE SKTEDLHDKD KDEKDDNFGG PSIRGFELFS SSPVRRAKKT
EQSGVNKHKD EKLLLEPLDL SLSLPDVLLP IGGQDTNQLG SPVRSGSVRS LTDTFCTNSD
GFTMSMSFSG SRSFNHNPSC SLSHNIGDNE QSVHSRPIFQ GIDWQALSHN DSKYNENTVY
QRLMENGNGS VQPRAMKGNL ISGQADEEHL RLPDGSSKAA NILEKQLSFQ KSVDVRSACP
RTGSLENGSK FTVEKKTAKD FYSGSNSWIT GLEAGGHDFV ETVIRYILSD SMPVMTKRFH
EMPTRNITSL KENIRQMMLN MDKNVQLGAF QDALQNRTDI TLELLTKSHR AQLEILVALK
SGRSDFLLLD NSISSSHLAE IFMNMRCKNL SCRVLLPVDE CDCRVCSRKD GFCSACMCLV
CSNFDMASNT CSWVGCDVCL HWCHTDCGIK ESYIRNGINA SGAPGMTEMQ FHCVACNHPS
EMFGFVKEVF LNFAREWKFE RFCKELEYVN KIFSSSKDSR GKQLRQAADA MLASLKSKLI
GLPEACNRIL GFISDCDSST PAETSAPFIY EQPKPRHERG SPSQDTAWLR SVCSDNPHNQ
LKRSASVADA FHRERQVEIC AVEMELERGS PKEPRFEELE SIVRMKQAEA EMFQGRADDA
RREAEGLKRI AIAKKEKIEE EYNRRMGKLS MEDAQERRRR RYEELEAMQR GQREFYEMKM
RMEEEMRGLL TKMEMTKQSL AL