ID   ARSA_HALSA              Reviewed;         644 AA.
AC   O52027;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Putative arsenical pump-driving ATPase;
DE            EC=7.3.2.7;
DE   AltName: Full=Arsenical resistance ATPase;
DE   AltName: Full=Arsenite-translocating ATPase;
DE   AltName: Full=Arsenite-transporting ATPase;
GN   Name=arsA; Synonyms=arsA2; OrderedLocusNames=VNG_5180G;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OG   Plasmid pNRC100.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=9847077; DOI=10.1101/gr.8.11.1131;
RA   Ng W.V., Ciufo S.A., Smith T.M., Bumgarner R.E., Baskin D., Faust J.,
RA   Hall B., Loretz C., Seto J., Slagel J., Hood L., DasSarma S.;
RT   "Snapshot of a large dynamic replicon in a halophilic archaeon: megaplasmid
RT   or minichromosome?";
RL   Genome Res. 8:1131-1141(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
RN   [3]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=15126481; DOI=10.1128/jb.186.10.3187-3194.2004;
RA   Wang G., Kennedy S.P., Fasiludeen S., Rensing C., DasSarma S.;
RT   "Arsenic resistance in Halobacterium sp. strain NRC-1 examined by using an
RT   improved gene knockout system.";
RL   J. Bacteriol. 186:3187-3194(2004).
CC   -!- FUNCTION: Anion-transporting ATPase. Catalyzes the extrusion of
CC       arsenite (By similarity). Involved in resistance to arsenite and
CC       antimonite but not to arsenate. {ECO:0000250,
CC       ECO:0000269|PubMed:15126481}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=arsenite(in) + ATP + H2O = ADP + arsenite(out) + H(+) +
CC         phosphate; Xref=Rhea:RHEA:11348, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.7;
CC   -!- INDUCTION: By arsenite and antimonite. {ECO:0000269|PubMed:15126481}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the arsADRC operon results in
CC       increased sensitivity to arsenite and antimonite but not arsenate.
CC       {ECO:0000269|PubMed:15126481}.
CC   -!- SIMILARITY: Belongs to the arsA ATPase family. {ECO:0000305}.
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DR   EMBL; AF016485; AAC82907.1; -; Genomic_DNA.
DR   PIR; T08340; T08340.
DR   RefSeq; WP_010890458.1; NZ_BK010830.1.
DR   AlphaFoldDB; O52027; -.
DR   SMR; O52027; -.
DR   EnsemblBacteria; AAC82907; AAC82907; AAC82907.
DR   GeneID; 5954920; -.
DR   GeneID; 62888362; -.
DR   KEGG; hal:arsA; -.
DR   HOGENOM; CLU_021619_0_0_2; -.
DR   InParanoid; O52027; -.
DR   OMA; KFVVMDT; -.
DR   OrthoDB; 84406at2157; -.
DR   PhylomeDB; O52027; -.
DR   Proteomes; UP000000554; Plasmid pNRC100.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0015446; F:ATPase-coupled arsenite transmembrane transporter activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR025723; Anion-transp_ATPase-like_dom.
DR   InterPro; IPR027541; Ars_ATPase.
DR   InterPro; IPR016300; ATPase_ArsA/GET3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10803; PTHR10803; 3.
DR   Pfam; PF02374; ArsA_ATPase; 3.
DR   PIRSF; PIRSF001327; Arsenical_pump-driving_ATPase; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00345; GET3_arsA_TRC40; 2.
PE   2: Evidence at transcript level;
KW   Arsenical resistance; ATP-binding; Nucleotide-binding; Plasmid;
KW   Reference proteome; Translocase.
FT   CHAIN           1..644
FT                   /note="Putative arsenical pump-driving ATPase"
FT                   /id="PRO_0000152259"
FT   BINDING         26..33
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         350..357
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   644 AA;  70532 MW;  4D93DDE76828C8C0 CRC64;
     MTATQTPAKE VVEPNSEDTE FVFFSGKGGV GKSTVSCATA TWLADNDYDT LLVTTDPAPN
     LSDIFNQDIG HEVTAIDDVP NLSAIEIDPD VAAEEYRQET IEPMRALLGD EEIQTVEEQL
     NSPCVEEIAA FDNFVDFMDS PEYDVVVFDT APTGHTIRLM ELPSDWNAEL EKGGSTCIGP
     AASMDDKKAD YERAIDTLSD ESRTSFAFVG KPESSSIDEI ERSASDLAEL GISSQLLVVN
     GYLPESVCED PFFEGKRADE QAVIDRVEST FDQQALATYP LQPGEIAGLE LLSDVGGVLY
     DGEEATVDVD AATRRATNED TVDFDTFTDA DAVAEELVPV EETRYLFFTG KGGVGKSTIA
     STTAVSLAEA GYETLVVTTD PAAHLADIFE QPVGHEPTSV GQANLDAARI DQERALEEYR
     TQVLDHVREM YDEKDDTQID VEAAVANVEE ELESPCAEEM AALEKFVSYF EEDGYDIVVF
     DTAPTGHTLR LLELPSDWKG FMDLGSLTKG AAPANGGKYD EVIETMQDPS RSSFAFVMYP
     EFTPMMEAYR AAMDLQDQVG IETSVVVANY LLPEDYGDNA FFENRRAQQA EYLEEISERF
     DVPMMLAPLR QEEPVGLDDL REFGADVTGL DGVGEDDREE VTVS