ARSA_METJA
ID ARSA_METJA Reviewed; 349 AA.
AC Q58542;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Putative arsenical pump-driving ATPase;
DE EC=7.3.2.7;
DE AltName: Full=Arsenical resistance ATPase;
DE AltName: Full=Arsenite-translocating ATPase;
DE AltName: Full=Arsenite-transporting ATPase;
GN OrderedLocusNames=MJ1142;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: Anion-transporting ATPase. Catalyzes the extrusion of
CC arsenite (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=arsenite(in) + ATP + H2O = ADP + arsenite(out) + H(+) +
CC phosphate; Xref=Rhea:RHEA:11348, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.7;
CC -!- INTERACTION:
CC Q58542; Q58542: MJ1142; NbExp=3; IntAct=EBI-7584233, EBI-7584233;
CC -!- SIMILARITY: Belongs to the arsA ATPase family. {ECO:0000305}.
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DR EMBL; L77117; AAB99142.1; -; Genomic_DNA.
DR PIR; E64442; E64442.
DR RefSeq; WP_010870653.1; NC_000909.1.
DR PDB; 3UG6; X-ray; 3.30 A; A/B/C/D=1-349.
DR PDB; 3UG7; X-ray; 2.90 A; A/B/C/D=1-349.
DR PDBsum; 3UG6; -.
DR PDBsum; 3UG7; -.
DR AlphaFoldDB; Q58542; -.
DR SMR; Q58542; -.
DR IntAct; Q58542; 5.
DR MINT; Q58542; -.
DR STRING; 243232.MJ_1142; -.
DR TCDB; 3.A.19.1.3; the guided entry of tail anchored protein (get) family.
DR EnsemblBacteria; AAB99142; AAB99142; MJ_1142.
DR GeneID; 1452038; -.
DR KEGG; mja:MJ_1142; -.
DR eggNOG; arCOG02849; Archaea.
DR HOGENOM; CLU_040761_4_0_2; -.
DR InParanoid; Q58542; -.
DR OMA; MDAPYEF; -.
DR OrthoDB; 84406at2157; -.
DR PhylomeDB; Q58542; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0015446; F:ATPase-coupled arsenite transmembrane transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR025723; Anion-transp_ATPase-like_dom.
DR InterPro; IPR016300; ATPase_ArsA/GET3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10803; PTHR10803; 1.
DR Pfam; PF02374; ArsA_ATPase; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00345; GET3_arsA_TRC40; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Arsenical resistance; ATP-binding; Nucleotide-binding;
KW Reference proteome; Translocase.
FT CHAIN 1..349
FT /note="Putative arsenical pump-driving ATPase"
FT /id="PRO_0000152257"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 13..21
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 27..32
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 34..36
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 39..52
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 67..72
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 77..79
FT /evidence="ECO:0007829|PDB:3UG6"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 96..110
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 111..113
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 115..130
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 134..148
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 153..157
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 165..170
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 171..199
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 211..232
FT /evidence="ECO:0007829|PDB:3UG7"
FT TURN 235..237
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 238..244
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 248..263
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 268..276
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 284..303
FT /evidence="ECO:0007829|PDB:3UG7"
FT STRAND 307..313
FT /evidence="ECO:0007829|PDB:3UG7"
FT HELIX 322..332
FT /evidence="ECO:0007829|PDB:3UG7"
SQ SEQUENCE 349 AA; 39919 MW; 00041F603DFDD5A3 CRC64;
MLSKIKDSIN SLRGITEKKL EKKDGTKYIM FGGKGGVGKT TMSAATGVYL AEKGLKVVIV
STDPAHSLRD IFEQEFGHEP TKVKGYDNLY VVEIDPQKAM EEYKEKLKAQ IEENPFLGEM
LEDQLEMAAL SPGTDESAAF DVFLKYMDSN EFDVVIFDTA PTGHTLRFLG MPEVMDKYMT
KLIKLRKQMS GFMKMMKKLL PFGGKDEDID YDKMLEELEK MKERIVRARN ILSDPERTAF
RLVVIPEEMS ILESERAMKA LQKYGIPIDA VIVNQLIPED VQCDFCRARR ELQLKRLEMI
KEKFGDKVIA YVPLLRTEAK GIETLKQIAK ILYGEEEKEE QKIEQKVGQ
