ARSA_METTH
ID ARSA_METTH Reviewed; 324 AA.
AC O27555;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Putative arsenical pump-driving ATPase;
DE EC=7.3.2.7;
DE AltName: Full=Arsenical resistance ATPase;
DE AltName: Full=Arsenite-translocating ATPase;
DE AltName: Full=Arsenite-transporting ATPase;
GN OrderedLocusNames=MTH_1511;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Anion-transporting ATPase. Catalyzes the extrusion of
CC arsenite (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=arsenite(in) + ATP + H2O = ADP + arsenite(out) + H(+) +
CC phosphate; Xref=Rhea:RHEA:11348, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.7;
CC -!- SIMILARITY: Belongs to the arsA ATPase family. {ECO:0000305}.
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DR EMBL; AE000666; AAB85986.1; -; Genomic_DNA.
DR PIR; F69068; F69068.
DR RefSeq; WP_010877121.1; NC_000916.1.
DR PDB; 3ZQ6; X-ray; 2.11 A; A/B/C/D=1-324.
DR PDBsum; 3ZQ6; -.
DR AlphaFoldDB; O27555; -.
DR SMR; O27555; -.
DR STRING; 187420.MTH_1511; -.
DR EnsemblBacteria; AAB85986; AAB85986; MTH_1511.
DR GeneID; 24854616; -.
DR KEGG; mth:MTH_1511; -.
DR PATRIC; fig|187420.15.peg.1474; -.
DR HOGENOM; CLU_040761_4_0_2; -.
DR OMA; MDAPYEF; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0015446; F:ATPase-coupled arsenite transmembrane transporter activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR025723; Anion-transp_ATPase-like_dom.
DR InterPro; IPR016300; ATPase_ArsA/GET3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10803; PTHR10803; 1.
DR Pfam; PF02374; ArsA_ATPase; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00345; GET3_arsA_TRC40; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Arsenical resistance; ATP-binding; Nucleotide-binding;
KW Reference proteome; Translocase.
FT CHAIN 1..324
FT /note="Putative arsenical pump-driving ATPase"
FT /id="PRO_0000152258"
FT BINDING 21..28
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 3..6
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 15..22
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 27..40
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 45..49
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 56..60
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 82..92
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 107..114
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 121..136
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 140..145
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 149..180
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT TURN 181..186
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 193..217
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT TURN 220..222
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 223..229
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 233..248
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 253..261
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 269..288
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT TURN 289..291
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT STRAND 292..298
FT /evidence="ECO:0007829|PDB:3ZQ6"
FT HELIX 307..318
FT /evidence="ECO:0007829|PDB:3ZQ6"
SQ SEQUENCE 324 AA; 36544 MW; B7D0687E4E7D50C6 CRC64;
MAFKDLFKFN KGKTTFVFIG GKGGVGKTTI SAATALWMAR SGKKTLVIST DPAHSLSDSL
EREIGHTPTK ITENLYAVEI DPEVAMEEYQ AKLQEQAAMN PGMGLDMLQD QMDMASMSPG
IDEAAAFDQF LRYMTTDEYD IVIFDTAPTG HTLRLLSFPE IMDSWVGKMI KIRRQIGSMA
KAFKNILPFM GDEEEEDRAL QDMEATKKQI NAAREVMSDP ERTSFKMVVI PEEMSIYESE
RAMKALEKYS IHADGVIVNQ VLPEESDCEF CNARRKLQQE RLKQIREKFS DKVVAEVPLL
KKEAKGIETL EKIAEQLYGE PEPE
