ID   ARSB1_ECOLX             Reviewed;         429 AA.
AC   P08691;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Arsenical pump membrane protein;
GN   Name=arsB;
OS   Escherichia coli.
OG   Plasmid R773.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3021763; DOI=10.1016/s0021-9258(18)66824-3;
RA   Chen C.-M., Misra T.K., Silver S., Rosen B.P.;
RT   "Nucleotide sequence of the structural genes for an anion pump. The
RT   plasmid-encoded arsenical resistance operon.";
RL   J. Biol. Chem. 261:15030-15038(1986).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=2523997; DOI=10.1111/j.1365-2958.1989.tb00098.x;
RA   San Francisco M.J., Tisa L.S., Rosen B.P.;
RT   "Identification of the membrane component of the anion pump encoded by the
RT   arsenical resistance operon of R-factor R773.";
RL   Mol. Microbiol. 3:15-21(1989).
RN   [3]
RP   FUNCTION.
RX   PubMed=1688427; DOI=10.1016/s0021-9258(19)40214-7;
RA   Tisa L.S., Rosen B.P.;
RT   "Molecular characterization of an anion pump. The ArsB protein is the
RT   membrane anchor for the ArsA protein.";
RL   J. Biol. Chem. 265:190-194(1990).
RN   [4]
RP   REVIEW.
RX   PubMed=1704144; DOI=10.1016/0923-2508(90)90008-e;
RA   Rosen B.P.;
RT   "The plasmid-encoded arsenical resistance pump: an anion-translocating
RT   ATPase.";
RL   Res. Microbiol. 141:336-341(1990).
CC   -!- FUNCTION: Involved in arsenical resistance. Thought to form the channel
CC       of an arsenite pump. {ECO:0000269|PubMed:1688427}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:2523997};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:2523997}.
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DR   EMBL; J02591; AAA21095.1; -; Genomic_DNA.
DR   PIR; B25937; B25937.
DR   RefSeq; WP_063116263.1; NZ_QOXP01000024.1.
DR   AlphaFoldDB; P08691; -.
DR   SMR; P08691; -.
DR   DIP; DIP-16996N; -.
DR   PATRIC; fig|562.10854.peg.1380; -.
DR   BioCyc; MetaCyc:MON-21685; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015105; F:arsenite transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
DR   CDD; cd01118; ArsB_permease; 1.
DR   InterPro; IPR000802; Arsenical_pump_ArsB.
DR   Pfam; PF02040; ArsB; 1.
DR   PRINTS; PR00758; ARSENICPUMP.
DR   TIGRFAMs; TIGR00935; 2a45; 1.
PE   4: Predicted;
KW   Arsenical resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Plasmid; Transmembrane; Transmembrane helix.
FT   CHAIN           1..429
FT                   /note="Arsenical pump membrane protein"
FT                   /id="PRO_0000064660"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        316..335
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   429 AA;  45599 MW;  60626D3B8269955F CRC64;
     MLLAGAIFIL TIVLVIWQPK GLGIGWSATL GAVLALASGV IHIADIPVVW NIVWNATATF
     IAVIIISLLL DESGFFEWAA LHVSRWGNGR GRLLFTYIVL LGAAVAALFA NDGAALILTP
     IVIAMLLALG FSKSTTLAFV MAAGFISDTA SLPLIVSNLV NIVSADFFKL GFTEYASVMV
     PVDIAAIIAT LVMLHLFFRK DIPPTYELAR LKEPAKAIKD PATFRTGWVV LLLLLVGFFV
     LEPMGIPVSA IAAVGAAVLF AVAKKGHGIN TGKVLRGAPW QIVIFSLGMY LVIYGLRNAG
     LTDYLSDVLN ELADKGLWAA TLGTGFLTAL LSSIMNNMPT VLIGALSIDG STATGVIKEA
     MIYANVIGCD LGPKITPIGS LATLLWLHVL SQKNMTITWG YYFRTGIVMT LPVLFVTLAA
     LALRLSVTL