ID   ARSB2_ECOLX             Reviewed;         429 AA.
AC   P52146;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Arsenical pump membrane protein;
GN   Name=arsB;
OS   Escherichia coli.
OG   Plasmid IncN R46.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8674982; DOI=10.1111/j.1574-6968.1996.tb08195.x;
RA   Bruhn D.F., Li J., Silver S., Roberto F., Rosen B.P.;
RT   "The arsenical resistance operon of IncN plasmid R46.";
RL   FEMS Microbiol. Lett. 139:149-153(1996).
CC   -!- FUNCTION: Involved in arsenical resistance. Thought to form the channel
CC       of an arsenite pump.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
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DR   EMBL; U38947; AAB09627.1; -; Genomic_DNA.
DR   AlphaFoldDB; P52146; -.
DR   SMR; P52146; -.
DR   IntAct; P52146; 1.
DR   STRING; 585034.ECIAI1_3649; -.
DR   eggNOG; COG1055; Bacteria.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015105; F:arsenite transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
DR   CDD; cd01118; ArsB_permease; 1.
DR   InterPro; IPR000802; Arsenical_pump_ArsB.
DR   Pfam; PF02040; ArsB; 1.
DR   PRINTS; PR00758; ARSENICPUMP.
DR   TIGRFAMs; TIGR00935; 2a45; 1.
PE   4: Predicted;
KW   Arsenical resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Plasmid; Transmembrane; Transmembrane helix.
FT   CHAIN           1..429
FT                   /note="Arsenical pump membrane protein"
FT                   /id="PRO_0000064661"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        316..336
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   429 AA;  45447 MW;  A08E7CB735B4D0F0 CRC64;
     MLLAGAIFVL TIVLVIWQPK GLGIGWSATL GAVLALISGV VHIGDIPVVW NIVWNATATF
     IAVIIISLLL DESGFFEWAA LHVSRWGNGR GRLLFTYIVL LGAAVAALFA NDGAALILTP
     IVIAMLLALG FSKGTTLAFV MAAGFIADTA SLPLIVSNLV NIVSADFFGL GFTEYASVMV
     PVDIAAIIAT LVMLHLFFRK DIPPTYDLAL LKAPAKAIKD LATFRTGWIV LILLLVGFFV
     LEPLGIPVSA IAAVGAVILF AVANRGHAIN TGKVLRGAPW QIVIFSLGMY LVVYGLRNAG
     LTEYLSGVLN VLADKGLWAA TFGTGFLTAF LSSIMNNMPT VLVGALSIDG STATGVIKEA
     MIFANVIGCD LGPKITPIGS LATLLWLHVL SQKNMTITWG YYFRTGIVMT LPVLFVTLAA
     LALRLSFTL