ARSB_BACSU
ID ARSB_BACSU Reviewed; 346 AA.
AC P45946;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Arsenite resistance protein ArsB;
GN Name=arsB; Synonyms=yqcL; OrderedLocusNames=BSU25790;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=7704261; DOI=10.1099/13500872-141-2-323;
RA Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.;
RT "Complete nucleotide sequence of a skin element excised by DNA
RT rearrangement during sporulation in Bacillus subtilis.";
RL Microbiology 141:323-327(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 152.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP IDENTIFICATION.
RX PubMed=7489895; DOI=10.1016/0378-1119(95)00636-k;
RA Medigue C., Moszer I., Viari A., Danchin A.;
RT "Analysis of a Bacillus subtilis genome fragment using a co-operative
RT computer system prototype.";
RL Gene 165:GC37-GC51(1995).
RN [6]
RP ROLE IN ARSENIC RESISTANCE.
RX PubMed=9537360; DOI=10.1128/jb.180.7.1655-1661.1998;
RA Sato T., Kobayashi Y.;
RT "The ars operon in the skin element of Bacillus subtilis confers resistance
RT to arsenate and arsenite.";
RL J. Bacteriol. 180:1655-1661(1998).
CC -!- FUNCTION: Seems to confer resistance to arsenite by allowing cells to
CC extrude this compound. Could be part of an arsenite extrusion pump.
CC {ECO:0000269|PubMed:9537360}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Deletion of the arsB gene results in both an
CC aresenite- and arsenate-sensitive phenotype in the absence of IPTG.
CC -!- SIMILARITY: Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59)
CC family. {ECO:0000305}.
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DR EMBL; D32216; BAA06969.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12433.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14520.2; -; Genomic_DNA.
DR PIR; B69950; B69950.
DR RefSeq; NP_390456.2; NC_000964.3.
DR RefSeq; WP_004398718.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P45946; -.
DR SMR; P45946; -.
DR STRING; 224308.BSU25790; -.
DR TCDB; 2.A.59.1.2; the arsenical resistance-3 (acr3) family.
DR PaxDb; P45946; -.
DR PRIDE; P45946; -.
DR EnsemblBacteria; CAB14520; CAB14520; BSU_25790.
DR GeneID; 937800; -.
DR KEGG; bsu:BSU25790; -.
DR PATRIC; fig|224308.179.peg.2803; -.
DR eggNOG; COG0798; Bacteria.
DR InParanoid; P45946; -.
DR OMA; CTAMVLM; -.
DR PhylomeDB; P45946; -.
DR BioCyc; BSUB:BSU25790-MON; -.
DR BioCyc; MetaCyc:BSU25790-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015104; F:antimonite transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0015105; F:arsenite transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015699; P:antimonite transport; IBA:GO_Central.
DR GO; GO:0015700; P:arsenite transport; IBA:GO_Central.
DR GO; GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR004706; Arsenical-R_Acr3.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR PANTHER; PTHR43057; PTHR43057; 1.
DR Pfam; PF01758; SBF; 1.
DR PIRSF; PIRSF005508; Acr3; 1.
DR TIGRFAMs; TIGR00832; acr3; 1.
PE 3: Inferred from homology;
KW Arsenical resistance; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..346
FT /note="Arsenite resistance protein ArsB"
FT /id="PRO_0000201482"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 152
FT /note="F -> S (in Ref. 1; BAA06969 and 2; BAA12433)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 346 AA; 38312 MW; 05739D0B768A8644 CRC64;
MKRLSFLDRY LTIWIFLAMA LGIGLGFIFP SFVGGLNKLQ VGTTSIPLAI GLVLMMYPPL
AKVRYEEIGR VFKDIKVLIL SLVQNWIIGP TLMFILAIIF LPDKPEYMIG LIMIGLARCI
AMVIVWNDLS KGDTEYAAGL VAFNSIFQML FFSVYAYIFV TVIPQWLGME GAVVNITMAE
VAKSVFIYLG VPFIAGMVTR YIFVKVKGKE WYEKVFIPKI SPITLIALLF TIIVMFSLKG
DVIVSLPLDV VRVAIPLLIY FVLMFFVSFF LGKKIGANYA VTTTLAFTAG SNNFELAIAV
AVGVFGIHSG AAFAAVIGPL VEVPVMIALV KVALWFQRKY FGSHSM
