ARSB_ECO57
ID ARSB_ECO57 Reviewed; 429 AA.
AC P0AB94; P37310; P76708;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Arsenical pump membrane protein;
DE AltName: Full=Arsenic efflux pump protein;
GN Name=arsB; OrderedLocusNames=Z4904, ECs4374;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Involved in arsenical resistance. Thought to form the channel
CC of an arsenite pump (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ArsB family. {ECO:0000305}.
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DR EMBL; AE005174; AAG58634.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37797.1; -; Genomic_DNA.
DR PIR; F86021; F86021.
DR PIR; F91175; F91175.
DR RefSeq; NP_312401.1; NC_002695.1.
DR RefSeq; WP_000922639.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AB94; -.
DR SMR; P0AB94; -.
DR STRING; 155864.EDL933_4742; -.
DR EnsemblBacteria; AAG58634; AAG58634; Z4904.
DR EnsemblBacteria; BAB37797; BAB37797; ECs_4374.
DR GeneID; 66672615; -.
DR GeneID; 915771; -.
DR KEGG; ece:Z4904; -.
DR KEGG; ecs:ECs_4374; -.
DR PATRIC; fig|386585.9.peg.4570; -.
DR eggNOG; COG1055; Bacteria.
DR HOGENOM; CLU_043931_1_0_6; -.
DR OMA; SWGYYFK; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015105; F:arsenite transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
DR CDD; cd01118; ArsB_permease; 1.
DR InterPro; IPR000802; Arsenical_pump_ArsB.
DR Pfam; PF02040; ArsB; 1.
DR PRINTS; PR00758; ARSENICPUMP.
DR TIGRFAMs; TIGR00935; 2a45; 1.
PE 3: Inferred from homology;
KW Arsenical resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..429
FT /note="Arsenical pump membrane protein"
FT /id="PRO_0000201468"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..45
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..97
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..120
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..177
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..227
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..310
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..429
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 429 AA; 45497 MW; 66EB1C03E479B6E9 CRC64;
MLLAGAIFVL TIVLVIWQPK GLGIGWSATL GAVLALVTGV VHPGDIPVVW NIVWNATAAF
IAVIIISLLL DESGFFEWAA LHVSRWGNGR GRLLFTWIVL LGAAVAALFA NDGAALILTP
IVIAMLLALG FSKGTTLAFV MAAGFIADTA SLPLIVSNLV NIVSADFFGL GFREYASVMV
PVDIAAIVAT LVMLHLYFRK DIPQNYDMAL LKSPAEAIKD PATFKTGWVV LLLLLVGFFV
LEPLGIPVSA IAAVGALILF VVAKRGHAIN TGKVLRGAPW QIVIFSLGMY LVVYGLRNAG
LTEYLSGVLN VLADNGLWAA TLGTGFLTAF LSSIMNNMPT VLVGALSIDG STASGVIKEA
MVYANVIGCD LGPKITPIGS LATLLWLHVL SQKNMTISWG YYFRTGIIMT LPVLFVTLAA
LALRLSFTL
