ARSB_ECOLI
ID ARSB_ECOLI Reviewed; 429 AA.
AC P0AB93; P37310; P76708; Q2M7G4;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Arsenical pump membrane protein;
DE AltName: Full=Arsenic efflux pump protein;
GN Name=arsB; Synonyms=arsF; OrderedLocusNames=b3502, JW3469;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7721697; DOI=10.1128/jb.177.8.2050-2056.1995;
RA Diorio C., Cai J., Marmor J., Shinder R., Dubow M.S.;
RT "An Escherichia coli chromosomal ars operon homolog is functional in
RT arsenic detoxification and is conserved in Gram-negative bacteria.";
RL J. Bacteriol. 177:2050-2056(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY.
RX PubMed=1535622; DOI=10.1016/s0021-9258(18)42315-0;
RA Wu J., Tisa L.S., Rosen B.P.;
RT "Membrane topology of the ArsB protein, the membrane subunit of an anion-
RT translocating ATPase.";
RL J. Biol. Chem. 267:12570-12576(1992).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in arsenical resistance. Thought to form the channel
CC of an arsenite pump (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ArsB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB18478.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X80057; CAA56362.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18478.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76527.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77792.1; -; Genomic_DNA.
DR PIR; S47723; S47723.
DR RefSeq; NP_417959.4; NC_000913.3.
DR RefSeq; WP_000922639.1; NZ_SSZK01000042.1.
DR AlphaFoldDB; P0AB93; -.
DR SMR; P0AB93; -.
DR BioGRID; 4261325; 7.
DR IntAct; P0AB93; 1.
DR STRING; 511145.b3502; -.
DR TCDB; 3.A.4.1.1; the arsenite-antimonite (arsab) efflux family.
DR PaxDb; P0AB93; -.
DR PRIDE; P0AB93; -.
DR EnsemblBacteria; AAC76527; AAC76527; b3502.
DR EnsemblBacteria; BAE77792; BAE77792; BAE77792.
DR GeneID; 66672615; -.
DR GeneID; 948011; -.
DR KEGG; ecj:JW3469; -.
DR KEGG; eco:b3502; -.
DR PATRIC; fig|511145.12.peg.3606; -.
DR EchoBASE; EB2148; -.
DR eggNOG; COG1055; Bacteria.
DR HOGENOM; CLU_043931_1_0_6; -.
DR InParanoid; P0AB93; -.
DR OMA; SWGYYFK; -.
DR PhylomeDB; P0AB93; -.
DR BioCyc; EcoCyc:ARSF-MON; -.
DR BioCyc; MetaCyc:ARSF-MON; -.
DR BRENDA; 7.3.2.7; 2026.
DR PRO; PR:P0AB93; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoliWiki.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0042960; F:antimonite secondary active transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0008490; F:arsenite secondary active transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015699; P:antimonite transport; IDA:EcoCyc.
DR GO; GO:0015700; P:arsenite transport; IDA:EcoCyc.
DR GO; GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
DR CDD; cd01118; ArsB_permease; 1.
DR InterPro; IPR000802; Arsenical_pump_ArsB.
DR Pfam; PF02040; ArsB; 1.
DR PRINTS; PR00758; ARSENICPUMP.
DR TIGRFAMs; TIGR00935; 2a45; 1.
PE 1: Evidence at protein level;
KW Arsenical resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..429
FT /note="Arsenical pump membrane protein"
FT /id="PRO_0000201467"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..45
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..97
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..120
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..177
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..227
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..310
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..429
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 429 AA; 45497 MW; 66EB1C03E479B6E9 CRC64;
MLLAGAIFVL TIVLVIWQPK GLGIGWSATL GAVLALVTGV VHPGDIPVVW NIVWNATAAF
IAVIIISLLL DESGFFEWAA LHVSRWGNGR GRLLFTWIVL LGAAVAALFA NDGAALILTP
IVIAMLLALG FSKGTTLAFV MAAGFIADTA SLPLIVSNLV NIVSADFFGL GFREYASVMV
PVDIAAIVAT LVMLHLYFRK DIPQNYDMAL LKSPAEAIKD PATFKTGWVV LLLLLVGFFV
LEPLGIPVSA IAAVGALILF VVAKRGHAIN TGKVLRGAPW QIVIFSLGMY LVVYGLRNAG
LTEYLSGVLN VLADNGLWAA TLGTGFLTAF LSSIMNNMPT VLVGALSIDG STASGVIKEA
MVYANVIGCD LGPKITPIGS LATLLWLHVL SQKNMTISWG YYFRTGIIMT LPVLFVTLAA
LALRLSFTL
