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OBLC_ASPCL
ID   OBLC_ASPCL              Reviewed;         482 AA.
AC   A1C8C5;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=FAD-dependent oxidoreductase oblC {ECO:0000303|PubMed:27116000};
DE            EC=1.21.-.- {ECO:0000269|PubMed:27116000};
DE   AltName: Full=Ophiobolin biosynthesis cluster protein C {ECO:0000303|PubMed:27116000};
DE   Flags: Precursor;
GN   Name=oblC {ECO:0000303|PubMed:27116000}; ORFNames=ACLA_076870;
OS   Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 /
OS   NRRL 1 / QM 1276 / 107).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=344612;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
RN   [2]
RP   FUNCTION.
RX   PubMed=23324037; DOI=10.1021/ol303408a;
RA   Chiba R., Minami A., Gomi K., Oikawa H.;
RT   "Identification of ophiobolin F synthase by a genome mining approach: a
RT   sesterterpene synthase from Aspergillus clavatus.";
RL   Org. Lett. 15:594-597(2013).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=27116000; DOI=10.1021/acs.orglett.6b00552;
RA   Narita K., Chiba R., Minami A., Kodama M., Fujii I., Gomi K., Oikawa H.;
RT   "Multiple oxidative modifications in the ophiobolin biosynthesis: P450
RT   oxidations found in genome mining.";
RL   Org. Lett. 18:1980-1983(2016).
CC   -!- FUNCTION: FAD-dependent oxidoreductase; part of the gene cluster that
CC       mediates the biosynthesis of the sesterterpenes ophiobolins, fungal
CC       phytotoxins with potential anti-cancer activities (PubMed:23324037,
CC       PubMed:27116000). The first step of the pathway is performed by the
CC       sesterterpene synthase oblA that possesses both prenyl transferase and
CC       terpene cyclase activity, converting isopentenyl diphosphate and
CC       dimethylallyl diphosphate into geranylfarnesyl diphosphate (GFPP) and
CC       further converting GFPP into ophiobolin F, respectively
CC       (PubMed:23324037). Other sesterterpenoids (C(25) terpenoids) are found
CC       as minor products of oblA (PubMed:23324037). It is expected that
CC       ophiobolin F is then oxidized to ophiobolin A via ophiobolin C and
CC       ophiobolin B intermediates by the combined action of the cytochrome
CC       P450 monooxygenase oblB and the FAD-dependent oxidoreductase oblC
CC       (Probable). Although oblB catalyzes multistep oxygenations at C5 and
CC       C21/C7 in a relatively efficient manner, it is unable to convert
CC       ophiobolin F to ophiobolin C and produces instead several unexpected
CC       derivatives (PubMed:27116000). {ECO:0000269|PubMed:23324037,
CC       ECO:0000269|PubMed:27116000, ECO:0000305|PubMed:27116000}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:B8NI10};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000305|PubMed:27116000}.
CC   -!- SIMILARITY: Belongs to the beta-cyclopiazonate dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; DS027045; EAW14646.1; -; Genomic_DNA.
DR   RefSeq; XP_001276072.1; XM_001276071.1.
DR   AlphaFoldDB; A1C8C5; -.
DR   SMR; A1C8C5; -.
DR   EnsemblFungi; EAW14646; EAW14646; ACLA_076870.
DR   GeneID; 4708208; -.
DR   KEGG; act:ACLA_076870; -.
DR   VEuPathDB; FungiDB:ACLA_076870; -.
DR   eggNOG; ENOG502R1TU; Eukaryota.
DR   HOGENOM; CLU_028280_0_0_1; -.
DR   OMA; EWHSHAP; -.
DR   OrthoDB; 569857at2759; -.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000006701; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Glycoprotein; Oxidoreductase; Reference proteome;
KW   Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..482
FT                   /note="FAD-dependent oxidoreductase oblC"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5002632992"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        165
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        238
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        337
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   482 AA;  52840 MW;  596381939B513157 CRC64;
     MHLSFGSLFA CLLASSTAVA SAPPSNFDVA NFKSADIIKR DVAVIGGGSA GTYSAISLKD
     KGKSVILIEK KGRLGGHAET YIDPATGTPV DMGVLVFHNI TVVKDYFKRF DIPLNRADDF
     MKSEFYFDFR TGRAVTPTFT PQAEEVSAAF AAYLAQYSKY PGLRNGTILP SPVPEELYMP
     FGKFVEKYGI QAAIPSMYYF NPGVGDILSN PVIEQFRYWS SDMVRSVATG FLGPVRRNVS
     ELYTRAEAEL FQSSSLLLSS EVVHALRADG ESGVMLIVRT PAGRKLILAK KLLIAFPPKL
     DFLAPFDLSA TEKSLFGKYI DAGYYVGLVK NSGLPNNTSI SNAAQGHTDF TFPYLPAAYS
     FAPSAVPGLQ LVTYATGQTT KSLPLSDEAV KADIINTIKR IQKQNPDKFT STEPEFVTFA
     SHAPYNLQVR AEDIRDGFYD KLYTLQGQRN THWTGAAWKG EDSSLLWTYS EEIVVPQVIE
     GL
 
 
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