OBLD_EMEVA
ID OBLD_EMEVA Reviewed; 1473 AA.
AC A0A1V1GB10;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2017, sequence version 1.
DT 03-AUG-2022, entry version 19.
DE RecName: Full=ABC-type transporter oblD {ECO:0000303|PubMed:27116000};
DE AltName: Full=Ophiobolin biosynthesis cluster protein D {ECO:0000303|PubMed:27116000};
GN Name=oblD {ECO:0000303|PubMed:27116000};
OS Emericella variicolor (Aspergillus stellatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=1549217;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=GF10;
RX PubMed=27116000; DOI=10.1021/acs.orglett.6b00552;
RA Narita K., Chiba R., Minami A., Kodama M., Fujii I., Gomi K., Oikawa H.;
RT "Multiple oxidative modifications in the ophiobolin biosynthesis: P450
RT oxidations found in genome mining.";
RL Org. Lett. 18:1980-1983(2016).
CC -!- FUNCTION: ABC-type transporter; part of the gene cluster that mediates
CC the biosynthesis of the sesterterpenes ophiobolins, fungal phytotoxins
CC with potential anti-cancer activities (PubMed:27116000). Acts as a
CC specific transporter involved in ophiobolins secretion (By similarity).
CC {ECO:0000250|UniProtKB:M2UCE5, ECO:0000269|PubMed:27116000}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:27116000};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCG family.
CC PDR (TC 3.A.1.205) subfamily. {ECO:0000305}.
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DR EMBL; LC127211; BAX09285.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V1GB10; -.
DR SMR; A0A1V1GB10; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR CDD; cd03233; ABCG_PDR_domain1; 1.
DR CDD; cd03232; ABCG_PDR_domain2; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013525; ABC_2_trans.
DR InterPro; IPR029481; ABC_trans_N.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR043926; ABCG_dom.
DR InterPro; IPR034001; ABCG_PDR_1.
DR InterPro; IPR034003; ABCG_PDR_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010929; PDR_CDR_ABC.
DR Pfam; PF01061; ABC2_membrane; 2.
DR Pfam; PF19055; ABC2_membrane_7; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR Pfam; PF14510; ABC_trans_N; 1.
DR Pfam; PF06422; PDR_CDR; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Glycoprotein; Membrane; Nucleotide-binding;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1473
FT /note="ABC-type transporter oblD"
FT /id="PRO_0000451175"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 514..534
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 560..580
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 589..609
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 622..642
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 733..753
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1153..1173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1189..1209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1234..1254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1277..1297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1312..1332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1425..1445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 115..370
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 808..1050
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 844..851
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 294
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 319
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1371
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1470
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 1473 AA; 164454 MW; DBC253FFFAC5552F CRC64;
MTDFNAIDLD EGTSKGSPSP EKRDAEVQQL AKQYTQQSAS SIYGQNPFCA DAGSALDPNG
DNFSARAWCK AMLQLQTEDD KAHPPRTLGV AFGNLNVHGF GSDTDYQKSV GNVWLQALGL
LRKALPGQRQ RKIDILHGLD GLVEAGEMLV VLGPPGSGCS TFLKTIAGET HGFYIDKASH
LNYQGATAKH IANDFRGEAI YTAEVDVHFP RLTVGDTLFF AARARAPRHI PGGVTINEYA
AHMRDVIMAM FGISHTRDTI IGNDFVRGVS GGERKRVTIA EASLSMAPLQ CWDNSTRGLD
SANAIEFCKT LRMQADINNT TACVSLYQAP QAAYDNFDKV LVLYKGRQIY FGPTATAKQY
FLDMGFECPD RQTDADFLTS MTSPKERVVR PGSEHQVPHT PDEFAARWKD STERARLLGA
IEAYNAKFSD GQQLDKFRQS RRAQQAKIQR VSSPYTLSYT QQIQLCLWRG WLRLKADPSI
TISSLFGNSI VALILGSMFY DLNGDTSSFF QRGALLFFAV LINALGCGLE MLTLYAQRGI
VEKHARYALY HPSSEAFASM LMDLPYKILN TIIFNLILYF MTNLRREAGA FFFFLFVNFI
LTLTMSMFFR SIASLTRTLV QALPAAAIFI LGLTMYTGFA IPTDYMLGWA KWIRFINPIN
FGFESLMVNE FHNRDFPCVN FVPSGLGYAD IGSGNQVCST VGSVAGQGFV NGDAYVESLY
DYTNSHKWRN VGIIFVFMIG LMFVYLAATE LILEKRSKGE ILVFRHGHSA LTGKSHDDEE
DAGGRTVAMA EKTESDDLAI IERQTAIVQW KDVCYDIKVG KENRRILDHI DGWVKPGTLT
ALMGVSGAGK TTLLDALATR TTMGVISGEI LVDGKQRDES FQRKTGYAQQ QDLHLSTSTV
REALTFSAVL RQPAHVPRQE KVDYVTEVIK LLDMTEYADA VIGVPGEGLN VEQRKRLTIG
VELAARPALL LFLDEPTSGL DSQTSWSILD LLDKLKKNGQ AILCTIHQPS AMLFQRFDRL
LFLKSGGQTV YFGDVGENSH ILIDYFVRNG GRPCPPAANP AEWMLEVIGA APGSHTDIDW
FSTWRKSPEY ARVQEHLQEL KLERSQLTDL ARTESGQKRQ NKADYREFAA PFYEQLCEVQ
YRVFQQLWRS PMYIYSKTVL CSSSALFVGF SLFRSPNSIQ GCQNQLFSIF MLLTIFGQLI
QQIMPHFVAQ RDLYEVRERP AKTYSWKAFM VSNILVELPW NSLMSLIMFL CWYYPVGLYR
NAEPTDAVTL RGAQMWLFIW AFLLFSSTFA HFMIAAFDSA ENAGNMGNLL YILCLLFCGV
LATPDQFPGF WIFMYRVSPF TYLVSGMLGV GLANTHVTCA ANEYLHFDPV NGSTCAEYME
AYRTTMGGYV LNGDATSDCS FCPMDDTNAY LATVSANYGD VWRNFGIMWV YIVFNAVAAC
ALYWWVRVPK GKKEAVETGA GAEAQKKTQN GSA
