OBSL1_MOUSE
ID OBSL1_MOUSE Reviewed; 1804 AA.
AC D3YYU8; F7AD47; Q80WA6;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Obscurin-like protein 1;
GN Name=Obsl1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Limb;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Core component of the 3M complex, a complex required to
CC regulate microtubule dynamics and genome integrity. It is unclear how
CC the 3M complex regulates microtubules, it could act by controlling the
CC level of a microtubule stabilizer. Acts as a regulator of the Cul7-
CC RING(FBXW8) ubiquitin-protein ligase, playing a critical role in the
CC ubiquitin ligase pathway that regulates Golgi morphogenesis and
CC dendrite patterning in brain. Required to localize CUL7 to the Golgi
CC apparatus in neurons (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the 3M complex, composed of core components CUL7,
CC CCDC8 and OBSL1. Interacts with CCDC8. Interacts with CUL7; the
CC interaction is direct. Interacts with FBXW8. Interacts (via N-terminal
CC Ig-like domain) with TTN/titin (via C-terminal Ig-like domain); the
CC interaction is direct (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O75147}.
CC Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O75147}. Golgi
CC apparatus {ECO:0000250|UniProtKB:O75147}. Note=Colocalizes with CUL7 at
CC the Golgi apparatus in neurons. {ECO:0000250|UniProtKB:O75147}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=D3YYU8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=D3YYU8-2; Sequence=VSP_055905, VSP_055906, VSP_055907;
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DR EMBL; AC115011; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC051643; AAH51643.1; -; mRNA.
DR CCDS; CCDS15077.2; -. [D3YYU8-1]
DR RefSeq; NP_849215.3; NM_178884.5.
DR AlphaFoldDB; D3YYU8; -.
DR SMR; D3YYU8; -.
DR BioGRID; 221115; 2.
DR STRING; 10090.ENSMUSP00000109197; -.
DR iPTMnet; D3YYU8; -.
DR PhosphoSitePlus; D3YYU8; -.
DR MaxQB; D3YYU8; -.
DR PaxDb; D3YYU8; -.
DR PeptideAtlas; D3YYU8; -.
DR PRIDE; D3YYU8; -.
DR ProteomicsDB; 294060; -. [D3YYU8-1]
DR ProteomicsDB; 294061; -. [D3YYU8-2]
DR Antibodypedia; 52509; 16 antibodies from 7 providers.
DR DNASU; 98733; -.
DR GeneID; 98733; -.
DR KEGG; mmu:98733; -.
DR UCSC; uc007bpn.2; mouse. [D3YYU8-1]
DR UCSC; uc007bpp.3; mouse. [D3YYU8-2]
DR CTD; 23363; -.
DR MGI; MGI:2138628; Obsl1.
DR VEuPathDB; HostDB:ENSMUSG00000026211; -.
DR eggNOG; KOG0613; Eukaryota.
DR HOGENOM; CLU_000630_0_0_1; -.
DR InParanoid; D3YYU8; -.
DR OrthoDB; 15947at2759; -.
DR PhylomeDB; D3YYU8; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR BioGRID-ORCS; 98733; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Obsl1; mouse.
DR PRO; PR:D3YYU8; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; D3YYU8; protein.
DR ExpressionAtlas; D3YYU8; baseline and differential.
DR Genevisible; D3YYU8; MM.
DR GO; GO:1990393; C:3M complex; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0007030; P:Golgi organization; ISO:MGI.
DR GO; GO:0007156; P:homophilic cell adhesion via plasma membrane adhesion molecules; IBA:GO_Central.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:0050775; P:positive regulation of dendrite morphogenesis; ISO:MGI.
DR GO; GO:0034067; P:protein localization to Golgi apparatus; ISO:MGI.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; ISS:UniProtKB.
DR GO; GO:0010842; P:retina layer formation; IBA:GO_Central.
DR GO; GO:0007416; P:synapse assembly; IBA:GO_Central.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 19.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR Pfam; PF07679; I-set; 13.
DR SMART; SM00409; IG; 17.
DR SMART; SM00408; IGc2; 14.
DR SUPFAM; SSF48726; SSF48726; 17.
DR SUPFAM; SSF49265; SSF49265; 1.
DR PROSITE; PS50853; FN3; 1.
DR PROSITE; PS50835; IG_LIKE; 13.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Disulfide bond; Golgi apparatus;
KW Immunoglobulin domain; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..1804
FT /note="Obscurin-like protein 1"
FT /id="PRO_0000430250"
FT DOMAIN 12..100
FT /note="Ig-like 1"
FT DOMAIN 128..225
FT /note="Ig-like 2"
FT DOMAIN 241..330
FT /note="Ig-like 3"
FT DOMAIN 339..425
FT /note="Ig-like 4"
FT DOMAIN 517..615
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 720..800
FT /note="Ig-like 5"
FT DOMAIN 804..891
FT /note="Ig-like 6"
FT DOMAIN 902..982
FT /note="Ig-like 7"
FT DOMAIN 986..1075
FT /note="Ig-like 8"
FT DOMAIN 1078..1165
FT /note="Ig-like 9"
FT DOMAIN 1176..1261
FT /note="Ig-like 10"
FT DOMAIN 1266..1442
FT /note="Ig-like 11"
FT DOMAIN 1536..1621
FT /note="Ig-like 12"
FT DOMAIN 1625..1694
FT /note="Ig-like 13"
FT DOMAIN 1702..1798
FT /note="Ig-like 14"
FT REGION 17..19
FT /note="Interaction with TTN"
FT /evidence="ECO:0000250"
FT REGION 85..94
FT /note="Interaction with TTN"
FT /evidence="ECO:0000250"
FT REGION 227..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 10
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75147"
FT DISULFID 33..84
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 149..209
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 267..319
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 362..412
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 738..788
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 829..879
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 920..970
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 1011..1061
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 1103..1153
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 1195..1245
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 1289..1430
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 1558..1608
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 1..478
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_055905"
FT VAR_SEQ 985..1004
FT /note="EPPVRIIYPQDEVTLHAVSL -> GVGLSQPPESPEDNPEPQEC (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_055906"
FT VAR_SEQ 1005..1804
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_055907"
FT CONFLICT 786
FT /note="F -> I (in Ref. 2; AAH51643)"
FT /evidence="ECO:0000305"
FT CONFLICT 858
FT /note="K -> E (in Ref. 2; AAH51643)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1804 AA; 197937 MW; FDD14572E8FC10B2 CRC64;
MKAGSGDQGS PPCFLRFPRP VRVVSGAEAE LKCVVLGEPP PTVVWEKGGQ QLVASERLSF
PEDGAEHGLL LSGALPTDAG VYVCRARNAA GEAYAAAAVT VLEPPAPEPE PESSECPLPT
PGTGEGAPKF LTGPQSQWVL RGEEVVLTCQ VGGLPEPKLY WEKDGMALDE VWDSSHFKLE
PGRGASDEGA SLTLRILAAR LPDSGVYVCH ARNAHGHAQA GALLQVHQPR ESPPQDPDEN
PKPVLEPLKG APKTFWVNEG KHAKFRCYVM GKPEPEIEWH LEGRPLLPDR RRLMYRDRDG
GFVLKVLYCQ AKDRGLYVCA ARNSAGQTLS AVQLHVKEPR LRFTRPLQDV EGREHGIVVL
ECKVPNSRIP TAWFREDQRL LPCRKYEQIE EGAVRRLVIH KLKADDDGVY LCEMRGRVRT
VANVTVKGPI LKRLPRKLDV LEGENAVLLV ETQEAGVQGC WSRDGEDLPD TCQSSCGHMH
ALVLPGVTRE DAGEITFSLG NSRTTTLLRV KCVKHSPPGP PVMVEMFKGQ KNKVLLTWKP
PEPPPETSFI YRLERQEVGS DDWIQCFSIE KAGAVEVPGD CVPTEGDYHF RICTVSEHGR
SPHVVFNGSA HLVPTARLVS GLEDVQVYDG EDAVFSLDLS AIIQGSWFLN GEQLQSNEPE
GQVEPGALRY RIEQKGLQHR LILQAVKHRD SGALVGFSCP GVQDSAALTI QESSVHILSP
QDKVSLTFTT SERVVLTCEL SRVDFPATWY KDGQKVEESE SLIVKTEGRK HRLILPEAQV
RDSGEFECRT EGVSAFFGVT VQDPPVHIVN PQEHVFVHAI TSECVRLTCE VDREDTTVHW
YKDGQEVEES DIIVLENKGP HHRLVLPAAR PSDGGEFQCV AGDERAYFTV TITDVFSWIV
YPSSEVHVAA VRLERVVLTC ELCRPWAEVR WTKDGEEVVE SPALLLEKED TIRRLVLPSV
QLEDSGEYLC EIHDESASFT ITVTEPPVRI IYPQDEVTLH AVSLECVVLT CELSREDAPV
RWYKDGLEVE ESEALVLQSD GPRRRLVLPA AQPEDGGEFV CDAGDDSAFF TVTVTAPPER
IVHPAARSLD LQFGAPGHVE LRCEVAPAGS QVRWYKDGLE VEVSDALQLG AEGPARTLTL
PHAQPEDAGE YVCETRDEAV TFNVSLAELP VQFLAPEAAP NPLCVVPGEP VVLSCELSRA
SAQVFWSHNG SPVQQGEGLE LRAEGPRRIL CIQAADLAHT GVYTCQSGAS PGAPSLSFNV
QVAELPPVKL VSELTPLTVH EGDDATFQCE VSPPDAEVTW LRNGAVITAG PQLEMVQNGS
SRTLIIRGCQ LKDAGTVTAR AGAADTSARL HVRETELLFL RRLQDVRAEE GQDVHLEVET
GRVGAAGTVR WIRGGEPLPL DSRLTTAQDG HVHRLSIHGV LLTDQGTYGC ESRHDRTLAR
LSVRPRQLRE LRPLEDVTVH EGGSATFQLE LSQEGVTGEW AQGGVRLHPG PKCHIQSEGR
THRLVLSGLG LADSGCVSFT ADTLRCAARL TVREVPVTIV QGPQDLEVTE GDTATFECEL
SQTLADVIWE KDGQALSLSP RLRLQALGTR RLLLLRRCCS SDAGTYSCVV GTARSEPARL
TVREREVSVL RELRSVSARE GDGATFECTV SETEITGRWE LGGRALRPGG RVRIRQEGKK
HILVLSELRT EDTGEVCFQA GPAQSLARLE VEALPLQMCR RPPREKTVLV NRRAVLEVTV
SRPGGHVCWM REGVELCPGN KYETRRHGTT HSLVIHDVRP EDQGTYSCQA GQDSADTQLL
VDGD
