OCAD1_DROME
ID OCAD1_DROME Reviewed; 257 AA.
AC Q9W1X9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=OCIA domain-containing protein 1;
DE Short=OCAD1 {ECO:0000250|UniProtKB:Q9CRD0};
GN Name=asrij; ORFNames=CG13533;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22110713; DOI=10.1371/journal.pone.0027667;
RA Kulkarni V., Khadilkar R.J., Magadi S.S., Srivathsa M.S., Inamdar M.S.;
RT "Asrij maintains the stem cell niche and controls differentiation during
RT Drosophila lymph gland hematopoiesis.";
RL PLoS ONE 6:E27667-E27667(2011).
RN [5]
RP FUNCTION, AND SUBUNIT.
RX PubMed=24707047; DOI=10.1073/pnas.1303559111;
RA Khadilkar R.J., Rodrigues D., Mote R.D., Sinha A.R., Kulkarni V.,
RA Magadi S.S., Inamdar M.S.;
RT "ARF1-GTP regulates Asrij to provide endocytic control of Drosophila blood
RT cell homeostasis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:4898-4903(2014).
CC -!- FUNCTION: Maintains stem cell potency (PubMed:24707047). Involved in
CC endocytic pathways that mediate signaling during hematopoiesis
CC (PubMed:24707047, PubMed:22110713). {ECO:0000269|PubMed:22110713,
CC ECO:0000269|PubMed:24707047}.
CC -!- SUBUNIT: Interacts with STAT3 and ARF1 (PubMed:24707047).
CC {ECO:0000269|PubMed:24707047}.
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000269|PubMed:22110713}.
CC -!- TISSUE SPECIFICITY: Expressed in all cells of the primary lymph gland
CC lobe. {ECO:0000269|PubMed:22110713}.
CC -!- MISCELLANEOUS: Marker for all hemocyte lineages during development.
CC {ECO:0000303|PubMed:22110713}.
CC -!- MISCELLANEOUS: 'Asrij' stands for 'blood' in Sanskrit as this protein
CC is strongly expressed in blood vessels.
CC -!- SIMILARITY: Belongs to the OCIAD1 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF46923.1; -; Genomic_DNA.
DR EMBL; AY089314; AAL90052.1; -; mRNA.
DR RefSeq; NP_611733.1; NM_137889.3.
DR AlphaFoldDB; Q9W1X9; -.
DR BioGRID; 63248; 5.
DR IntAct; Q9W1X9; 3.
DR STRING; 7227.FBpp0305129; -.
DR PaxDb; Q9W1X9; -.
DR PRIDE; Q9W1X9; -.
DR DNASU; 37637; -.
DR EnsemblMetazoa; FBtr0071902; FBpp0071813; FBgn0034793.
DR GeneID; 37637; -.
DR KEGG; dme:Dmel_CG13533; -.
DR UCSC; CG13533-RA; d. melanogaster.
DR CTD; 37637; -.
DR FlyBase; FBgn0034793; asrij.
DR VEuPathDB; VectorBase:FBgn0034793; -.
DR eggNOG; ENOG502RXQR; Eukaryota.
DR GeneTree; ENSGT00530000063690; -.
DR HOGENOM; CLU_083038_0_0_1; -.
DR InParanoid; Q9W1X9; -.
DR OMA; PEMTKTY; -.
DR OrthoDB; 1322104at2759; -.
DR PhylomeDB; Q9W1X9; -.
DR BioGRID-ORCS; 37637; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 37637; -.
DR PRO; PR:Q9W1X9; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034793; Expressed in secondary oocyte and 34 other tissues.
DR ExpressionAtlas; Q9W1X9; baseline and differential.
DR Genevisible; Q9W1X9; DM.
DR GO; GO:0005769; C:early endosome; IDA:FlyBase.
DR GO; GO:0005768; C:endosome; IDA:FlyBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0005764; C:lysosome; IDA:FlyBase.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0055037; C:recycling endosome; IDA:FlyBase.
DR GO; GO:0035162; P:embryonic hemopoiesis; IEP:FlyBase.
DR GO; GO:0006897; P:endocytosis; IMP:FlyBase.
DR GO; GO:0061484; P:hematopoietic stem cell homeostasis; IMP:FlyBase.
DR GO; GO:0035170; P:lymph gland crystal cell differentiation; IMP:FlyBase.
DR GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; IMP:FlyBase.
DR GO; GO:0002786; P:regulation of antibacterial peptide production; IMP:FlyBase.
DR GO; GO:0010821; P:regulation of mitochondrion organization; IMP:FlyBase.
DR GO; GO:2000736; P:regulation of stem cell differentiation; ISS:UniProtKB.
DR InterPro; IPR040187; OCAD1/2.
DR InterPro; IPR009764; OCIA_dom.
DR PANTHER; PTHR13336; PTHR13336; 1.
DR Pfam; PF07051; OCIA; 1.
PE 1: Evidence at protein level;
KW Endosome; Reference proteome.
FT CHAIN 1..257
FT /note="OCIA domain-containing protein 1"
FT /id="PRO_0000299389"
FT DOMAIN 1..110
FT /note="OCIA"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 148..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..169
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..257
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 257 AA; 28621 MW; C07E8734E8242061 CRC64;
MDSPLNDGSH HPPPHAPHPL ADYQFSAEEV KALRECNTES FFQRSLPFGT GLGLLAYFGV
KNGYLQGHVK YGAVPKVVMG VILGYFVGKF SYQQKCAEKI MRLPNSHLGE LLRQRRQGGG
VISSITPDEN LGRAFTLAPF SPSSADVYSD EAYQPGRSTS LNLDTESRPT LSGLDDIYRP
TLDSGSMLEA ELPLEPSKPG QSYEDLRRRN REEYSKHQQS PYSRPYEPPV AVQQRPVEQA
QSEPAGRKNQ YGDSWTD
