ID   OCLP1_APIME             Reviewed;          74 AA.
AC   H9KQJ7;
DT   22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Omega-conotoxin-like protein 1;
DE            Short=OCLP1;
DE   Flags: Precursor;
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DH4;
RG   Honey bee genome project;
RA   Zhang L., Deng J., Wu Y.-Q., Kovar C., Aqrawi P., Bandaranaike D.,
RA   Blankenburg K., Chen D., Denson S., Dinh H., Firestine M., Gross S.,
RA   Han Y., Hernandez B., Holder M., Jackson L., Javaid M., Jing C., Jones J.,
RA   Joshi V., Kamau G., Korchina V., Lee S., Lorensuhewa L., Mata R.,
RA   Mathew T., Mims S., Ngo R., Nguyen L., Okwuonu G., Ongeri F., Osuji N.,
RA   Pham C., Puazo M., Qu C., Quiroz J., Raj R., Rio Deiros D., Santibanez J.,
RA   Scheel M., Scherer S., Vee V., Wang M., Xin Y., Richards S., Reid J.G.,
RA   Newsham I., Worley K.C., Muzny D.M., Gibbs R.;
RL   Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   TISSUE SPECIFICITY, FUNCTION, AND BIOASSAY.
RX   PubMed=17433819; DOI=10.1016/j.jmb.2007.02.106;
RA   Kaplan N., Morpurgo N., Linial M.;
RT   "Novel families of toxin-like peptides in insects and mammals: a
RT   computational approach.";
RL   J. Mol. Biol. 369:553-566(2007).
RN   [3]
RP   TISSUE SPECIFICITY, AND POSSIBLE ANTIBACTERIAL ACTIVITY.
RX   PubMed=22442369; DOI=10.1242/jeb.065276;
RA   Gatschenberger H., Gimple O., Tautz J., Beier H.;
RT   "Honey bee drones maintain humoral immune competence throughout all life
RT   stages in the absence of vitellogenin production.";
RL   J. Exp. Biol. 215:1313-1322(2012).
RN   [4]
RP   IDENTIFICATION.
RC   STRAIN=DH4;
RG   EnsemblMetazoa;
RL   Submitted (AUG-2012) to UniProtKB.
CC   -!- FUNCTION: The impact of this protein on the neuronal activity of the
CC       honeybee brain is not known. It does not affect apparent movement or
CC       hatching of blowfly larvae. However, when injected into fish, it
CC       induces a strong reversible paralytic effect. In addition, the presence
CC       of this small peptide in the hemolymph of adult drones together with
CC       its induction after bacterial infection suggests that this peptide
CC       exhibits antibacterial activity. This peptide may act by inhibiting ion
CC       channels. {ECO:0000269|PubMed:17433819}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in brain. Is also found in
CC       hemolymph. {ECO:0000269|PubMed:17433819, ECO:0000269|PubMed:22442369}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
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DR   RefSeq; XP_006560077.1; XM_006560014.1.
DR   AlphaFoldDB; H9KQJ7; -.
DR   PaxDb; H9KQJ7; -.
DR   EnsemblMetazoa; XM_006560014; XP_006560077; LOC725074.
DR   GeneID; 725074; -.
DR   KEGG; ame:725074; -.
DR   HOGENOM; CLU_180299_0_0_1; -.
DR   InParanoid; H9KQJ7; -.
DR   OMA; CGRHGDP; -.
DR   OrthoDB; 1591435at2759; -.
DR   PhylomeDB; H9KQJ7; -.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Antibiotic; Antimicrobial; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Reference proteome; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..74
FT                   /note="Omega-conotoxin-like protein 1"
FT                   /id="PRO_0000425185"
FT   DISULFID        24..38
FT                   /evidence="ECO:0000250"
FT   DISULFID        31..43
FT                   /evidence="ECO:0000250"
FT   DISULFID        37..50
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   74 AA;  8298 MW;  0219086BFCA7206B CRC64;
     MSKFILLVCI LLLTTNIVSA ASKCGRHGDS CVSSSDCCPG TWCHTYANRC QVRITEEELM
     KQREKILGRK GKDY