OCT1_CAEEL
ID OCT1_CAEEL Reviewed; 585 AA.
AC Q9U539; O02270;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 3.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Organic cation transporter 1;
DE AltName: Full=CeOCT1;
GN Name=oct-1; ORFNames=F52F12.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), AND FUNCTION.
RX PubMed=10209228; DOI=10.1016/s0005-2736(99)00020-6;
RA Wu X., Fei Y.-J., Huang W., Chancy C., Leibach F.H., Ganapathy V.;
RT "Identity of the F52F12.1 gene product in Caenorhabditis elegans as an
RT organic cation transporter.";
RL Biochim. Biophys. Acta 1418:239-244(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-87 AND ASN-98, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Bristol N2;
RX PubMed=17761667; DOI=10.1074/mcp.m600392-mcp200;
RA Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T.,
RA Taoka M., Takahashi N., Isobe T.;
RT "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis
RT elegans and suggests an atypical translocation mechanism for integral
RT membrane proteins.";
RL Mol. Cell. Proteomics 6:2100-2109(2007).
CC -!- FUNCTION: Transports organic cations such as tetraethylammonium (TEA).
CC Displays a broad substrate specificity. {ECO:0000269|PubMed:10209228}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q9U539-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q9U539-2; Sequence=VSP_011122;
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AF110415; AAF21932.1; -; mRNA.
DR EMBL; Z83228; CAB05732.2; -; Genomic_DNA.
DR EMBL; Z83228; CAC70093.1; -; Genomic_DNA.
DR PIR; T22509; T22509.
DR RefSeq; NP_001021482.1; NM_001026311.4. [Q9U539-2]
DR RefSeq; NP_492617.4; NM_060216.5. [Q9U539-1]
DR AlphaFoldDB; Q9U539; -.
DR SMR; Q9U539; -.
DR BioGRID; 38264; 1.
DR IntAct; Q9U539; 1.
DR STRING; 6239.F52F12.1a; -.
DR TCDB; 2.A.1.19.37; the major facilitator superfamily (mfs).
DR iPTMnet; Q9U539; -.
DR EPD; Q9U539; -.
DR PaxDb; Q9U539; -.
DR EnsemblMetazoa; F52F12.1a.1; F52F12.1a.1; WBGene00003842. [Q9U539-1]
DR EnsemblMetazoa; F52F12.1b.1; F52F12.1b.1; WBGene00003842. [Q9U539-2]
DR GeneID; 172841; -.
DR KEGG; cel:CELE_F52F12.1; -.
DR UCSC; F52F12.1a; c. elegans. [Q9U539-1]
DR CTD; 172841; -.
DR WormBase; F52F12.1a; CE41244; WBGene00003842; oct-1. [Q9U539-1]
DR WormBase; F52F12.1b; CE29329; WBGene00003842; oct-1. [Q9U539-2]
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000173250; -.
DR InParanoid; Q9U539; -.
DR OMA; PFIICME; -.
DR OrthoDB; 655566at2759; -.
DR PhylomeDB; Q9U539; -.
DR Reactome; R-CEL-112311; Neurotransmitter clearance.
DR Reactome; R-CEL-181430; Norepinephrine Neurotransmitter Release Cycle.
DR Reactome; R-CEL-197264; Nicotinamide salvaging.
DR Reactome; R-CEL-200425; Carnitine metabolism.
DR Reactome; R-CEL-2161517; Abacavir transmembrane transport.
DR Reactome; R-CEL-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-CEL-549127; Organic cation transport.
DR Reactome; R-CEL-561048; Organic anion transport.
DR Reactome; R-CEL-9749641; Aspirin ADME.
DR PRO; PR:Q9U539; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00003842; Expressed in adult organism and 1 other tissue.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0015101; F:organic cation transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0015695; P:organic cation transport; IMP:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..585
FT /note="Organic cation transporter 1"
FT /id="PRO_0000220513"
FT TOPO_DOM 1..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..155
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..184
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..269
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..360
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..389
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..446
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 468..476
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 498..504
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 526..585
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 544..585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 563..585
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17761667"
FT CARBOHYD 98
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17761667"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..17
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000303|PubMed:10209228"
FT /id="VSP_011122"
SQ SEQUENCE 585 AA; 65462 MW; 20DA0B3E73386B62 CRC64;
MSFQAMETFA EISQEILMSA TKPPDFDFVL EQVGNYGTYQ IVFFFIICLP TSLPSAFSAF
NIPFVVGNPP HTCHIPEGKE YLRPLTNDTQ ILSCKQYNET QINVFRAFTS APVDTYSDRI
SLVPCQNGWD YDNSTYLDSL VTEFNLVCDQ QAWIEISTTS FYVGSFIGNC LFGYVADKFG
RRRSFFVILT VLIVCGTASS FAKDIESFII LRFFTGLAFP ALFQIPFIIC MEFMGNSGRI
FSGLMTSLFF GAAMALLGVV AMFIRRWRQL TFFCNAPFAF YIIYYFFLPE SPRWSVSVGK
WADAKKQLKK IAKMNGKSNV DVDELVDSMK NHQNAAEEKE TKRSHNVTDL FKTPNLRRKT
LIVTYIWVMN AIIYNGLTLN VSNLPVDDYW SFIINGAVEL PGYFVVWPLL QCAGRRWTLA
ATMIVCGIGC VSAMFMPDGY PWLVASASFI GKFGVGSGFA VIYIFAGELY PTVVRAIGMG
MSSMVAGSGL LLAPHIVNLG KIVKILPLLI MGLMALSAGI LTFFLPETLG APLPMTIEDA
ENFGKKPEPD SGMFTQAAKK RESQPLLEPH TPMDRRRRSS RLMNI
