OCT2_ARATH
ID OCT2_ARATH Reviewed; 527 AA.
AC O64515;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Organic cation/carnitine transporter 2;
DE Short=AtOCT2;
GN Name=OCT2; Synonyms=2-Oct; OrderedLocusNames=At1g79360;
GN ORFNames=YUP8H12R.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY [LARGE SCALE ANALYSIS].
RX PubMed=16607029; DOI=10.1104/pp.105.074708;
RA Bock K.W., Honys D., Ward J.M., Padmanaban S., Nawrocki E.P., Hirschi K.D.,
RA Twell D., Sze H.;
RT "Integrating membrane transport with male gametophyte development and
RT function through transcriptomics.";
RL Plant Physiol. 140:1151-1168(2006).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=18710496; DOI=10.1186/1756-0500-1-43;
RA Kuefner I., Koch W.;
RT "Stress regulated members of the plant organic cation transporter family
RT are localized to the vacuolar membrane.";
RL BMC Res. Notes 1:43-43(2008).
RN [6]
RP TISSUE SPECIFICITY [LARGE SCALE ANALYSIS].
RX PubMed=18702670; DOI=10.1111/j.1365-313x.2008.03652.x;
RA Chaudhuri B., Hoermann F., Lalonde S., Brady S.M., Orlando D.A., Benfey P.,
RA Frommer W.B.;
RT "Protonophore- and pH-insensitive glucose and sucrose accumulation detected
RT by FRET nanosensors in Arabidopsis root tips.";
RL Plant J. 56:948-962(2008).
CC -!- FUNCTION: High affinity carnitine transporter involved in the active
CC cellular uptake of carnitine. Also transports organic cations (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:18710496};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18710496}.
CC -!- TISSUE SPECIFICITY: Weakly expressed in roots, including tips and
CC initiation site of lateral roots, siliques and flowers, especially in
CC pollen and stigma. {ECO:0000269|PubMed:16607029,
CC ECO:0000269|PubMed:18702670, ECO:0000269|PubMed:18710496}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AC002986; AAC17057.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36234.1; -; Genomic_DNA.
DR PIR; T01019; T01019.
DR RefSeq; NP_178054.1; NM_106584.3.
DR AlphaFoldDB; O64515; -.
DR SMR; O64515; -.
DR STRING; 3702.AT1G79360.1; -.
DR PaxDb; O64515; -.
DR PRIDE; O64515; -.
DR ProteomicsDB; 250863; -.
DR EnsemblPlants; AT1G79360.1; AT1G79360.1; AT1G79360.
DR GeneID; 844274; -.
DR Gramene; AT1G79360.1; AT1G79360.1; AT1G79360.
DR KEGG; ath:AT1G79360; -.
DR Araport; AT1G79360; -.
DR TAIR; locus:2207360; AT1G79360.
DR eggNOG; KOG0255; Eukaryota.
DR HOGENOM; CLU_001265_33_5_1; -.
DR InParanoid; O64515; -.
DR OMA; QMRRNTL; -.
DR OrthoDB; 762280at2759; -.
DR PhylomeDB; O64515; -.
DR PRO; PR:O64515; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O64515; baseline and differential.
DR Genevisible; O64515; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Ion transport; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..527
FT /note="Organic cation/carnitine transporter 2"
FT /id="PRO_0000415358"
FT TOPO_DOM 1..27
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 28..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..119
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171..176
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..195
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..201
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..230
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348..356
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..385
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 407..415
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 437..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..469
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 470..475
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 497..527
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT BINDING 190..197
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 527 AA; 57750 MW; AB4B70C2314C53D6 CRC64;
MAEPTQPLLT DSNSSSPRSL DDTIESYIGS FGWAQFLQAA LVSFSGVFDA QQTFISVFTD
SEPTWHCTDS NSICHESISN ICILPKTAWS WDYSPHVSVI SEWGLQCAGS FVKGLPESSF
FVGCLIGGLV LSTLADSSLG RKNMLFLSCL VMAISTMLTV FSPNIWVYAV LRFVNGFGRA
TIGTCALVLS TELVGKKWRG RVGIMSFFGF MLGFLSLPLM AYMNRGSSWR ILYAWTSIPT
IIYCVLVRFF VCESPRWLFV RGRREEAISI LKRVASIPST DVSSGGAISM SFSSLPFEED
EEKPSTNVNI FTTMKVLVEK RWALKRLSAV MAIAFGIGLV YYGMPLALSN LDFNIYLSAA
FNALMDLPAN LITLFLVDKL SRRNALIGFT ALGGVSSVLI FALHNMRIGN HGALQLALEL
ISYFSACSAF NMEMIYTIEL FPTCVRNSAI AMARQALVLG GVFSPIMVAA GRKNAFWSFG
LFGLAIGLLG LFAVGLPETR GSDLCDTMDE EECKDRRSKV AVNNVIA
