OCT4_ARATH
ID OCT4_ARATH Reviewed; 526 AA.
AC Q9LHQ6;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Organic cation/carnitine transporter 4;
DE Short=AtOCT4;
GN Name=OCT4; Synonyms=4-Oct; OrderedLocusNames=At3g20660; ORFNames=F3H11.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY [LARGE SCALE ANALYSIS].
RX PubMed=16607029; DOI=10.1104/pp.105.074708;
RA Bock K.W., Honys D., Ward J.M., Padmanaban S., Nawrocki E.P., Hirschi K.D.,
RA Twell D., Sze H.;
RT "Integrating membrane transport with male gametophyte development and
RT function through transcriptomics.";
RL Plant Physiol. 140:1151-1168(2006).
RN [6]
RP SUBCELLULAR LOCATION, INDUCTION BY ABIOTIC STRESS, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=18710496; DOI=10.1186/1756-0500-1-43;
RA Kuefner I., Koch W.;
RT "Stress regulated members of the plant organic cation transporter family
RT are localized to the vacuolar membrane.";
RL BMC Res. Notes 1:43-43(2008).
CC -!- FUNCTION: High affinity carnitine transporter involved in the active
CC cellular uptake of carnitine. Also transports organic cations (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:18710496};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18710496}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in siliques, and, to a lower
CC extent, in stems, leaves, flowers and siliques. Present in pollen. In
CC the stems of secondary inflorescences present in the phloem cells and
CC xylem parenchyma cells. {ECO:0000269|PubMed:16607029,
CC ECO:0000269|PubMed:18710496}.
CC -!- INDUCTION: During drought stress treatment.
CC {ECO:0000269|PubMed:18710496}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AP002034; BAB02242.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76409.1; -; Genomic_DNA.
DR EMBL; BT010744; AAR23714.1; -; mRNA.
DR EMBL; AK176424; BAD44187.1; -; mRNA.
DR RefSeq; NP_188702.2; NM_112957.3.
DR AlphaFoldDB; Q9LHQ6; -.
DR SMR; Q9LHQ6; -.
DR STRING; 3702.AT3G20660.1; -.
DR TCDB; 2.A.1.19.43; the major facilitator superfamily (mfs).
DR PaxDb; Q9LHQ6; -.
DR PRIDE; Q9LHQ6; -.
DR ProteomicsDB; 250865; -.
DR EnsemblPlants; AT3G20660.1; AT3G20660.1; AT3G20660.
DR GeneID; 821613; -.
DR Gramene; AT3G20660.1; AT3G20660.1; AT3G20660.
DR KEGG; ath:AT3G20660; -.
DR Araport; AT3G20660; -.
DR TAIR; locus:2083425; AT3G20660.
DR eggNOG; KOG0255; Eukaryota.
DR HOGENOM; CLU_001265_33_5_1; -.
DR InParanoid; Q9LHQ6; -.
DR OMA; YYMFAQV; -.
DR OrthoDB; 464838at2759; -.
DR PhylomeDB; Q9LHQ6; -.
DR PRO; PR:Q9LHQ6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LHQ6; baseline and differential.
DR Genevisible; Q9LHQ6; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042631; P:cellular response to water deprivation; IEP:UniProtKB.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Ion transport; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..526
FT /note="Organic cation/carnitine transporter 4"
FT /id="PRO_0000415360"
FT TOPO_DOM 1..52
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..153
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..179
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..240
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..360
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 412..418
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 440..451
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..475
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 497..526
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT BINDING 201..208
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 526 AA; 57502 MW; 24850869B1DC772B CRC64;
MESPEDRNGN DVRQPLLEKI PVKKEAEGEE RLCIDEMLQR YCGEFGRWQL KHFVLTCIAW
ALEAFHTMVM IFADQEPEWR CVGSDCRVGS LNCELDPSSW EWTAGKGSST VSEWGLICGD
KYKVGLVQAL FFAGCMIGAG VFGHLSDSKL GRKGSLTVVC IINAIFGIAT AFSPNYWTYV
VLRFLTGFST GGVGLTAFVL ATEPIGPSKR GVAGMSTFYF FSAGIAVLSG IAYVFRSWRE
LFIVSSLPSL LFLLIVIPFI SESPRWYLVR GKVDEAMKLM HSIAKTNGRH IPAGVTLALD
DDVENNNGER NTAVEGSLKD VILSPLMRMR LVISVAISFT VSIVYYGLSL NVGNLKTNLY
LNVFVNAVSE MPAFAITAVL LDKYGRKPLS IGTQWFSCVF CLVGFSVWGA GPWKSVRMVS
GVLGIFGMAG TYNLLFIYIA ELFPTVVRNA ALGCATQAAQ MGAILAPFVV VLGEELPFGV
FAVCGLVGGG LAFYLPETLN KPLYDTMFGM HEAESESNRE RGEVIC
