OCT6_ARATH
ID OCT6_ARATH Reviewed; 521 AA.
AC Q9SA36;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Organic cation/carnitine transporter 6;
DE Short=AtOCT6;
GN Name=OCT6; Synonyms=6-Oct; OrderedLocusNames=At1g16370; ORFNames=F3O9.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION, INDUCTION BY ABIOTIC STRESS, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=18710496; DOI=10.1186/1756-0500-1-43;
RA Kuefner I., Koch W.;
RT "Stress regulated members of the plant organic cation transporter family
RT are localized to the vacuolar membrane.";
RL BMC Res. Notes 1:43-43(2008).
CC -!- FUNCTION: High affinity carnitine transporter involved in the active
CC cellular uptake of carnitine. Also transports organic cations (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:18710496};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18710496}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and stems. In the stem of
CC secondary inflorescences, localized to the phloem. Also present in
CC flowers, specifically in the stamen, in the filaments and the
CC connective, and restricted to major veins in leaves.
CC {ECO:0000269|PubMed:18710496}.
CC -!- INDUCTION: During drought and salt stress treatments.
CC {ECO:0000269|PubMed:18710496}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AC006341; AAD34689.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29443.1; -; Genomic_DNA.
DR EMBL; AK229354; BAF01217.1; -; mRNA.
DR PIR; H86298; H86298.
DR RefSeq; NP_173087.1; NM_101503.4.
DR AlphaFoldDB; Q9SA36; -.
DR SMR; Q9SA36; -.
DR STRING; 3702.AT1G16370.1; -.
DR TCDB; 2.A.1.19.39; the major facilitator superfamily (mfs).
DR PaxDb; Q9SA36; -.
DR PRIDE; Q9SA36; -.
DR ProteomicsDB; 250866; -.
DR EnsemblPlants; AT1G16370.1; AT1G16370.1; AT1G16370.
DR GeneID; 838207; -.
DR Gramene; AT1G16370.1; AT1G16370.1; AT1G16370.
DR KEGG; ath:AT1G16370; -.
DR Araport; AT1G16370; -.
DR TAIR; locus:2032790; AT1G16370.
DR eggNOG; KOG0255; Eukaryota.
DR HOGENOM; CLU_001265_33_5_1; -.
DR InParanoid; Q9SA36; -.
DR OMA; MFLAFEF; -.
DR OrthoDB; 396963at2759; -.
DR PhylomeDB; Q9SA36; -.
DR PRO; PR:Q9SA36; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SA36; baseline and differential.
DR Genevisible; Q9SA36; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0071472; P:cellular response to salt stress; IEP:UniProtKB.
DR GO; GO:0042631; P:cellular response to water deprivation; IEP:UniProtKB.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Glycoprotein; Ion transport; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..521
FT /note="Organic cation/carnitine transporter 6"
FT /id="PRO_0000415362"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..177
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..182
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..200
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..213
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..241
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348..356
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..385
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 407..412
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 434..447
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 469..473
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 474..494
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 495..521
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT BINDING 200..207
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 521 AA; 58351 MW; 33FFB80C620A616E CRC64;
MADPISEPLL SHLTDDSGVN EKTRLEALTF DKIVEQSLSD FGFWQFFQIS LVGLALLFDA
QQIFITVYTD AYPTWHCLNH TICDPSASDI CKLPRSAWEW DGGSQGKSVI SEFGLECSSS
LLRGMPSSAF YIGAIVGGFF LALIPDDSLG RKKLVLFSTF AMSITSISVI FSTNVWIYTF
LKFIIGFSRS QTWSYALVLI SERVSTRWRP RATMIPFTLF VLGFMSLSGI AFLAQDSSWR
YLYLYTSVPA VFYCIFLYLF ALESPRWLHM QGKDKEAIDV LTKMSPKEKA YLESVVSKLP
LKQENFEQAP TYSIKDFFFR KWAFRRILVV MIIMFGLGIS YYGVPLAARD IDVNIYLSET
LNALVELPTF VITPILLERF NRRSSVLVNT LLGGASGVLC FVLSILGKTE IAFAFELGTF
FCARIGFNLM AVFMVEMFPT CVRSSATMMF RQALVVGGAC CPLIASIGRY IPSVSFAIFG
IAMSGLGMFV LILPETKGLS LCDSMEEQEK RDQAVNTSHV C
