OCTL_DROME
ID OCTL_DROME Reviewed; 567 AA.
AC Q95R48; Q9VCA3;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Organic cation transporter-like protein;
GN Name=Orct2; ORFNames=CG13610;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Probably transports organic cations. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAF56270.1; -; Genomic_DNA.
DR EMBL; AY061615; AAL29163.1; -; mRNA.
DR RefSeq; NP_001262907.1; NM_001275978.2.
DR RefSeq; NP_651238.2; NM_142981.3.
DR AlphaFoldDB; Q95R48; -.
DR SMR; Q95R48; -.
DR BioGRID; 67818; 3.
DR IntAct; Q95R48; 3.
DR STRING; 7227.FBpp0083982; -.
DR GlyGen; Q95R48; 4 sites.
DR PaxDb; Q95R48; -.
DR EnsemblMetazoa; FBtr0084598; FBpp0083982; FBgn0086365.
DR EnsemblMetazoa; FBtr0334869; FBpp0306892; FBgn0086365.
DR GeneID; 42890; -.
DR KEGG; dme:Dmel_CG13610; -.
DR CTD; 42890; -.
DR FlyBase; FBgn0086365; Orct2.
DR VEuPathDB; VectorBase:FBgn0086365; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000167832; -.
DR HOGENOM; CLU_001265_33_4_1; -.
DR InParanoid; Q95R48; -.
DR OMA; MGYDEVI; -.
DR OrthoDB; 704438at2759; -.
DR PhylomeDB; Q95R48; -.
DR Reactome; R-DME-112311; Neurotransmitter clearance.
DR Reactome; R-DME-181430; Norepinephrine Neurotransmitter Release Cycle.
DR Reactome; R-DME-197264; Nicotinamide salvaging.
DR Reactome; R-DME-200425; Carnitine metabolism.
DR Reactome; R-DME-2161517; Abacavir transmembrane transport.
DR Reactome; R-DME-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-DME-549127; Organic cation transport.
DR Reactome; R-DME-561048; Organic anion transport.
DR Reactome; R-DME-9749641; Aspirin ADME.
DR BioGRID-ORCS; 42890; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42890; -.
DR PRO; PR:Q95R48; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0086365; Expressed in head epidermis primordium (Drosophila) and 33 other tissues.
DR ExpressionAtlas; Q95R48; baseline and differential.
DR Genevisible; Q95R48; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015101; F:organic cation transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0015695; P:organic cation transport; ISS:UniProtKB.
DR GO; GO:0030307; P:positive regulation of cell growth; IMP:FlyBase.
DR GO; GO:0040018; P:positive regulation of multicellular organism growth; IMP:FlyBase.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..567
FT /note="Organic cation transporter-like protein"
FT /id="PRO_0000220515"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..127
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..244
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..371
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..427
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449..462
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 484..489
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 511..567
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 304..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 546..567
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 306..324
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..567
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 58..60
FT /note="SYD -> IYE (in Ref. 3; AAL29163)"
FT /evidence="ECO:0000305"
FT CONFLICT 410
FT /note="A -> T (in Ref. 3; AAL29163)"
FT /evidence="ECO:0000305"
FT CONFLICT 463
FT /note="L -> M (in Ref. 3; AAL29163)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 567 AA; 63167 MW; 0D9B979469395E60 CRC64;
MGYDEAIIHL GDFGRYQKII YFLICLTSIP VAFHKLAGVF LLAKPDFRCA LPFENGSSYD
LPTHLWNLSY PENERCSYYD VDYTEEYLNG SIPRSSNETK TCSSYVYDRS KYLNSAVTEW
NLVCGRDFMA ATSDSLFMLG VLLGSIVFGQ LSDKYGRKPI LFASLVIQVL FGVLAGVAPE
YFTYTFARLM VGATTSGVFL VAYVVAMEMV GPDKRLYAGI FVMMFFSVGF MLTAVFAYFV
HDWRWLQIAL TLPGLIFMFY YWIIPESARW LLLKGRKDCA IANMQKAARF NKVEISDEAL
SELLDEGENS EEKAKQKLED QELDEGPPPS VWDLFCYPNL RRKTLLIFLD WLVTSGVYYG
LSWNTSNLGG NVLLNFVISG AVEIPAYIFL LLTLNRWGRR SILCGCLVMA GLSLLATVII
PQRMHTLIVA CAMLGKLAIT ASYGTVYIFS AEQFPTVVRN VALGAASMVA RISGMMAPFL
NFLATIWKPL PLLICGSLTL VAGLLSLLLP ETHNKPMLET IADGERFGKK TKADVYLETG
QELRAPEAQP LKGSGETNGS TIANGHK
