ID   A28_MONPZ               Reviewed;         146 AA.
AC   Q8V4U9;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Envelope protein A28 homolog;
DE   AltName: Full=Protein A30;
GN   ORFNames=A30L;
OS   Monkeypox virus (strain Zaire-96-I-16) (MPX).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=619591;
OH   NCBI_TaxID=45479; Cynomys gunnisoni (Gunnison's prairie dog).
OH   NCBI_TaxID=99825; Cynomys leucurus (White-tailed prairie dog).
OH   NCBI_TaxID=45480; Cynomys ludovicianus (Black-tailed prairie dog).
OH   NCBI_TaxID=99826; Cynomys mexicanus (Mexican prairie dog).
OH   NCBI_TaxID=99827; Cynomys parvidens (Utah prairie dog).
OH   NCBI_TaxID=30650; Gliridae (dormice).
OH   NCBI_TaxID=226685; Heliosciurus ruwenzorii (Ruwenzori sun squirrel).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Zaire-96-I-16;
RX   PubMed=11734207; DOI=10.1016/s0014-5793(01)03144-1;
RA   Shchelkunov S.N., Totmenin A.V., Babkin I.V., Safronov P.F.,
RA   Ryazankina O.I., Petrov N.A., Gutorov V.V., Uvarova E.A., Mikheev M.V.,
RA   Sisler J.R., Esposito J.J., Jahrling P.B., Moss B., Sandakhchiev L.S.;
RT   "Human monkeypox and smallpox viruses: genomic comparison.";
RL   FEBS Lett. 509:66-70(2001).
CC   -!- FUNCTION: Envelope protein required for virus entry into host cell and
CC       for cell-cell fusion (syncytium formation). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       II membrane protein {ECO:0000305}. Note=Component of the intracellular
CC       mature virion (IMV) membrane. {ECO:0000250}.
CC   -!- PTM: Contains two intramolecular disulfide bonds. They are created by
CC       the viral disulfide bond formation pathway, a poxvirus-specific pathway
CC       that operates on the cytoplasmic side of the MV membranes (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae A28 protein family.
CC       {ECO:0000305}.
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DR   EMBL; AF380138; AAL40598.1; -; Genomic_DNA.
DR   RefSeq; NP_536567.1; NC_003310.1.
DR   GeneID; 928965; -.
DR   KEGG; vg:928965; -.
DR   Proteomes; UP000101269; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039663; P:membrane fusion involved in viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR007664; Poxvirus_A28.
DR   Pfam; PF04584; Pox_A28; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Fusion of virus membrane with host membrane; Late protein;
KW   Membrane; Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral envelope protein; Viral penetration into host cytoplasm; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..146
FT                   /note="Envelope protein A28 homolog"
FT                   /id="PRO_0000099294"
FT   TRANSMEM        1..21
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        22..146
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   146 AA;  16404 MW;  BCF80B33853228A5 CRC64;
     MNSLSIFFIV VATAAVCLLF IQSYSIYENY GNIKEFNATH AAFEYSKSIG GTPALDRRVQ
     DVNDTISDVK QKWRCVVYPG NGFVSASIFG FQAEVGPNNT RSIRKFNTMR QCIDFTFSDV
     INIDIYNPCI APNINNTECQ FLKSVL