ODFP4_MOUSE
ID ODFP4_MOUSE Reviewed; 290 AA.
AC Q8VI88; Q6P8Z7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Outer dense fiber protein 4;
DE AltName: Full=Outer dense fiber of sperm tails protein 4;
DE AltName: Full=Testis-specific protein oppo 1;
GN Name=Odf4; Synonyms=Oppo1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Testis;
RX PubMed=12079992; DOI=10.1095/biolreprod67.1.1;
RA Nakamura Y., Tanaka H., Koga M., Miyagawa Y., Iguchi N.,
RA Egydio de Carvalho C., Yomogida K., Nozaki M., Nojima H., Matsumiya K.,
RA Okuyama A., Nishimune Y.;
RT "Molecular cloning and characterization of Oppo 1: a haploid germ cell-
RT specific complementary DNA encoding sperm tail protein.";
RL Biol. Reprod. 67:1-7(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the outer dense fibers (ODF) of spermatozoa
CC which could be involved in sperm tail structure, sperm movement and
CC general organization of cellular cytoskeleton.
CC {ECO:0000269|PubMed:12079992}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VI88-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VI88-2; Sequence=VSP_028117, VSP_028118;
CC -!- TISSUE SPECIFICITY: Expressed in testis. {ECO:0000269|PubMed:12079992}.
CC -!- DEVELOPMENTAL STAGE: Expressed from late pachytene stage and expression
CC persists during embryonic development. {ECO:0000269|PubMed:12079992}.
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DR EMBL; AB074438; BAB83865.1; -; mRNA.
DR EMBL; AL603662; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC060992; AAH60992.1; -; mRNA.
DR CCDS; CCDS24872.1; -. [Q8VI88-1]
DR RefSeq; NP_665689.1; NM_145746.2. [Q8VI88-1]
DR AlphaFoldDB; Q8VI88; -.
DR STRING; 10090.ENSMUSP00000040050; -.
DR iPTMnet; Q8VI88; -.
DR PhosphoSitePlus; Q8VI88; -.
DR PaxDb; Q8VI88; -.
DR PRIDE; Q8VI88; -.
DR ProteomicsDB; 294268; -. [Q8VI88-1]
DR ProteomicsDB; 294269; -. [Q8VI88-2]
DR Ensembl; ENSMUST00000038932; ENSMUSP00000040050; ENSMUSG00000032921. [Q8VI88-1]
DR Ensembl; ENSMUST00000130271; ENSMUSP00000134383; ENSMUSG00000032921. [Q8VI88-2]
DR GeneID; 252868; -.
DR KEGG; mmu:252868; -.
DR UCSC; uc007joj.2; mouse. [Q8VI88-1]
DR CTD; 146852; -.
DR MGI; MGI:2182079; Odf4.
DR VEuPathDB; HostDB:ENSMUSG00000032921; -.
DR eggNOG; ENOG502SXVG; Eukaryota.
DR GeneTree; ENSGT01030000234855; -.
DR HOGENOM; CLU_062754_0_0_1; -.
DR InParanoid; Q8VI88; -.
DR OMA; RWPVDVS; -.
DR OrthoDB; 1432634at2759; -.
DR PhylomeDB; Q8VI88; -.
DR TreeFam; TF338228; -.
DR BioGRID-ORCS; 252868; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Odf4; mouse.
DR PRO; PR:Q8VI88; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8VI88; protein.
DR Bgee; ENSMUSG00000032921; Expressed in spermatocyte and 21 other tissues.
DR ExpressionAtlas; Q8VI88; baseline and differential.
DR Genevisible; Q8VI88; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031514; C:motile cilium; IDA:MGI.
DR GO; GO:0001520; C:outer dense fiber; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; Membrane;
KW Phosphoprotein; Reference proteome; Spermatogenesis; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..290
FT /note="Outer dense fiber protein 4"
FT /id="PRO_0000304723"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 247..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..265
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 28
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 174..178
FT /note="VTLIF -> GAPQL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028117"
FT VAR_SEQ 179..290
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028118"
FT CONFLICT 16
FT /note="Missing (in Ref. 3; AAH60992)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 290 AA; 33177 MW; BB59B7FBFF83D520 CRC64;
MEPDLNEEES ERIRTSRNRR SLEHRRNSLL PFQWKATNNS RWMAQVVASE FSLVAFLLLL
VMVFSKKWLY PSKSRFHQRY PQNVTKRVYT SIHSMSTGLL YICVSKSCPS SDNGEDNFKM
WTIHPVFGVA KISFTLAIGL GFVLTTWLHL PYLPCLQRMP FFGLIGIILS FCEVTLIFLT
LLLFPVNLWI YELRKNISVP IGWSYFIGWL VLILYFTCGI LCYLNHKNYW SLIMSSTTIN
TACSSLGPES LVSPSQTPSS QENSQESPKD DQKPSSPDKV VSPPQPDTTG
