ODO1_PETHY
ID ODO1_PETHY Reviewed; 294 AA.
AC Q50EX6;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=MYB-like transcription factor ODO1 {ECO:0000303|PubMed:15805488};
DE AltName: Full=Protein ODORANT1 {ECO:0000303|PubMed:15805488};
DE Short=PhODO1 {ECO:0000303|PubMed:22345641};
GN Name=ODO1 {ECO:0000303|PubMed:15805488};
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION DURING SCENT EMISSION.
RC STRAIN=cv. W115;
RX PubMed=15805488; DOI=10.1105/tpc.104.028837;
RA Verdonk J.C., Haring M.A., van Tunen A.J., Schuurink R.C.;
RT "ODORANT1 regulates fragrance biosynthesis in petunia flowers.";
RL Plant Cell 17:1612-1624(2005).
RN [2]
RP FUNCTION.
RX PubMed=21497087; DOI=10.1016/j.cub.2011.03.059;
RA Klahre U., Gurba A., Hermann K., Saxenhofer M., Bossolini E., Guerin P.M.,
RA Kuhlemeier C.;
RT "Pollinator choice in Petunia depends on two major genetic Loci for floral
RT scent production.";
RL Curr. Biol. 21:730-739(2011).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY EOBII.
RC STRAIN=cv. Mitchell, cv. R27, and cv. Violet 26;
RX PubMed=21585571; DOI=10.1111/j.1365-313x.2011.04644.x;
RA Van Moerkercke A., Haring M.A., Schuurink R.C.;
RT "The transcription factor EMISSION OF BENZENOIDS II activates the MYB
RT ODORANT1 promoter at a MYB binding site specific for fragrant petunias.";
RL Plant J. 67:917-928(2011).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION DURING SCENT EMISSION, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Mitchell, and cv. R27;
RX PubMed=22345641; DOI=10.1093/jxb/ers034;
RA Van Moerkercke A., Galvan-Ampudia C.S., Verdonk J.C., Haring M.A.,
RA Schuurink R.C.;
RT "Regulators of floral fragrance production and their target genes in
RT petunia are not exclusively active in the epidermal cells of petals.";
RL J. Exp. Bot. 63:3157-3171(2012).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY EOBI, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. W115;
RX PubMed=23275577; DOI=10.1105/tpc.112.105247;
RA Spitzer-Rimon B., Farhi M., Albo B., Cna'ani A., Ben Zvi M.M., Masci T.,
RA Edelbaum O., Yu Y., Shklarman E., Ovadis M., Vainstein A.;
RT "The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII,
RT regulates scent production by activating ODO1 and structural scent-related
RT genes in petunia.";
RL Plant Cell 24:5089-5105(2012).
RN [6]
RP INDUCTION BY EOBII.
RC STRAIN=cv. Mitchell, and cv. R27;
RX PubMed=22499185; DOI=10.4161/psb.19311;
RA Van Moerkercke A., Haring M.A., Schuurink R.C.;
RT "A model for combinatorial regulation of the petunia R2R3-MYB transcription
RT factor ODORANT1.";
RL Plant Signal. Behav. 7:518-520(2012).
RN [7]
RP REPRESSION BY LHY.
RX PubMed=26124104; DOI=10.1073/pnas.1422875112;
RA Fenske M.P., Hewett Hazelton K.D., Hempton A.K., Shim J.S., Yamamoto B.M.,
RA Riffell J.A., Imaizumi T.;
RT "Circadian clock gene LATE ELONGATED HYPOCOTYL directly regulates the
RT timing of floral scent emission in Petunia.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:9775-9780(2015).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Mitchell; TISSUE=Corolla;
RX PubMed=26620524; DOI=10.1104/pp.15.01646;
RA Shaipulah N.F.M., Muhlemann J.K., Woodworth B.D., Van Moerkercke A.,
RA Verdonk J.C., Ramirez A.A., Haring M.A., Dudareva N., Schuurink R.C.;
RT "CCoAOMT down-regulation activates anthocyanin biosynthesis in petunia.";
RL Plant Physiol. 170:717-731(2016).
CC -!- FUNCTION: R2R3 MYB-type transcription factor controlling the production
CC of volatile organic compounds (VOCs), including floral volatile
CC benzenoids and phenylpropanoids (FVBP), in flowers of fragrant
CC cultivars (e.g. cv. Mitchell and cv. V26) by regulating the shikimate
CC pathway, via the activation of several genes (e.g. EPSPS, ADT1, PAL1,
CC CFAT and CCoAOMT1) (PubMed:15805488, PubMed:21497087, PubMed:21585571,
CC PubMed:26620524). This scent, mostly produced in the evening and night
CC by the petals, attracts the pollinators (e.g. the night-active hawkmoth
CC pollinator Manduca sexta) (PubMed:15805488, PubMed:21497087). Promotes
CC the expression of ABCG1 in petals three hours before the onset of
CC volatile scent emission (PubMed:22345641). Anthocyanins production is
CC not controlled by ODO1 as color and scent are produced at different
CC stages of development (PubMed:15805488). Seems to trigger a negative
CC feed-back loop that represses the expression of EOBI (PubMed:23275577).
CC {ECO:0000269|PubMed:15805488, ECO:0000269|PubMed:21497087,
CC ECO:0000269|PubMed:21585571, ECO:0000269|PubMed:22345641,
CC ECO:0000269|PubMed:23275577, ECO:0000269|PubMed:26620524}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:22345641}.
CC -!- TISSUE SPECIFICITY: Restricted to the petals, with the highest
CC expression in the limb, probably in both epidermal and mesophyll cell
CC layers. {ECO:0000269|PubMed:15805488, ECO:0000269|PubMed:21585571,
CC ECO:0000269|PubMed:22345641}.
CC -!- DEVELOPMENTAL STAGE: Expressed in mature flowers and decreases upon
CC pollination. {ECO:0000269|PubMed:15805488}.
CC -!- INDUCTION: In fragrant cultivars (e.g. cv. Mitchell and cv. V26),
CC increases before the onset of volatile emission at the end of the light
CC period, peaks at night and decreases when volatile emission declines
CC early morning; this precise expression regulation of its promoter is
CC tuned by EOBI and EOBII binding and activation to a MYB binding site
CC (MBS) 5'-AAACCTAAT-3' and by LHY binding and repression to cis-
CC regulatory evening elements. {ECO:0000269|PubMed:15805488,
CC ECO:0000269|PubMed:21585571, ECO:0000269|PubMed:22345641,
CC ECO:0000269|PubMed:22499185, ECO:0000269|PubMed:23275577,
CC ECO:0000269|PubMed:26124104}.
CC -!- DISRUPTION PHENOTYPE: Reduced expression of genes involved in the
CC biosynthesis of precursors from the shikimate pathway (e.g. PAL1,
CC DAHPS, EPSPS, CCoAOMT1 and CM) thus leading to a lower accumulation of
CC volatile benzenoids but seems to not affect the production of
CC anthocyanins implicated in flower color (PubMed:15805488,
CC PubMed:26620524). Increased levels of EOBI (PubMed:23275577).
CC {ECO:0000269|PubMed:15805488, ECO:0000269|PubMed:23275577,
CC ECO:0000269|PubMed:26620524}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The life of a whiff - Issue
CC 60 of July 2005;
CC URL="https://web.expasy.org/spotlight/back_issues/060";
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DR EMBL; AY705977; AAV98200.1; -; mRNA.
DR AlphaFoldDB; Q50EX6; -.
DR SMR; Q50EX6; -.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0009423; P:chorismate biosynthetic process; IMP:UniProtKB.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Activator; Biological rhythms; DNA-binding; Nucleus; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..294
FT /note="MYB-like transcription factor ODO1"
FT /id="PRO_0000197081"
FT DOMAIN 9..61
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 62..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 128..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..152
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 294 AA; 33090 MW; 0C9FE79309EBADD8 CRC64;
MGRQPCCDKL GVKKGPWTAE EDKKLISFIL TNGQCCWRAV PKLAGLKRCG KSCRLRWTNY
LRPDLKRGLL SDAEEKLVID LHSRLGNRWS KIAARLPGRT DNEIKNHWNT HIKKKLLKMG
IDPVTHEPLK KEANLSDQPT TESDQNKENG HQQVQVVPQS TNVTAAAATS TEFDNNSSFS
SSASSSENSS CTTDESKLVF DNLSENDPLL SCLLEADTPL IDSPWEFPMS STTTVEEPKS
FDSIISNMTS WEDTFNWLSG YQEFGINDFG FDNCFNHVEL DIFKTIDNVE NRHG
