OE64M_ARATH
ID OE64M_ARATH Reviewed; 603 AA.
AC F4KCL7; Q9FY73;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Outer envelope protein 64, mitochondrial;
DE AltName: Full=Mitochondrial outer membrane protein 64;
DE Short=mtOM64;
DE AltName: Full=Translocon at the outer membrane of chloroplasts 64-V;
DE Short=AtTOC64-V;
GN Name=OM64; Synonyms=TOC64-V; OrderedLocusNames=At5g09420;
GN ORFNames=T5E8.220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=14741350; DOI=10.1016/s0014-5793(03)01457-1;
RA Chew O., Lister R., Qbadou S., Heazlewood J.L., Soll J., Schleiff E.,
RA Millar A.H., Whelan J.;
RT "A plant outer mitochondrial membrane protein with high amino acid sequence
RT identity to a chloroplast protein import receptor.";
RL FEBS Lett. 557:109-114(2004).
RN [4]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17655652; DOI=10.1111/j.1365-313x.2007.03207.x;
RA Aronsson H., Boij P., Patel R., Wardle A., Toepel M., Jarvis P.;
RT "Toc64/OEP64 is not essential for the efficient import of proteins into
RT chloroplasts in Arabidopsis thaliana.";
RL Plant J. 52:53-68(2007).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [6]
RP 3D-STRUCTURE MODELING.
RX PubMed=19198901; DOI=10.1007/s00894-008-0449-y;
RA Mirus O., Bionda T., von Haeseler A., Schleiff E.;
RT "Evolutionarily evolved discriminators in the 3-TPR domain of the Toc64
RT family involved in protein translocation at the outer membrane of
RT chloroplasts and mitochondria.";
RL J. Mol. Model. 15:971-982(2009).
CC -!- FUNCTION: Chaperone receptor mediating Hsp90-dependent protein
CC targeting to mitochondria. {ECO:0000250}.
CC -!- INTERACTION:
CC F4KCL7; Q9LHE5: TOM40-1; NbExp=2; IntAct=EBI-2124066, EBI-2124038;
CC F4KCL7; O80413: 103627788; Xeno; NbExp=2; IntAct=EBI-2124066, EBI-2362258;
CC F4KCL7; Q07185: AOX1; Xeno; NbExp=2; IntAct=EBI-2124066, EBI-2123914;
CC F4KCL7; Q7DM06; Xeno; NbExp=2; IntAct=EBI-2124066, EBI-2124012;
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000305};
CC Single-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and flower buds. Detected in
CC leaves. {ECO:0000269|PubMed:14741350, ECO:0000269|PubMed:17655652}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:17655652}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC05468.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL391712; CAC05468.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91391.1; -; Genomic_DNA.
DR RefSeq; NP_196504.2; NM_120979.3.
DR PDB; 6HPG; X-ray; 2.00 A; A/B/C/D/E/F=483-603.
DR PDB; 6Q3Q; X-ray; 2.00 A; A/B=483-603.
DR PDBsum; 6HPG; -.
DR PDBsum; 6Q3Q; -.
DR AlphaFoldDB; F4KCL7; -.
DR SMR; F4KCL7; -.
DR BioGRID; 16079; 9.
DR IntAct; F4KCL7; 5.
DR STRING; 3702.AT5G09420.1; -.
DR iPTMnet; F4KCL7; -.
DR SwissPalm; F4KCL7; -.
DR PaxDb; F4KCL7; -.
DR PRIDE; F4KCL7; -.
DR ProteomicsDB; 239016; -.
DR EnsemblPlants; AT5G09420.1; AT5G09420.1; AT5G09420.
DR GeneID; 830801; -.
DR Gramene; AT5G09420.1; AT5G09420.1; AT5G09420.
DR KEGG; ath:AT5G09420; -.
DR Araport; AT5G09420; -.
DR TAIR; locus:2184757; AT5G09420.
DR eggNOG; KOG1124; Eukaryota.
DR eggNOG; KOG1211; Eukaryota.
DR HOGENOM; CLU_009600_17_1_1; -.
DR InParanoid; F4KCL7; -.
DR OMA; TGENSHY; -.
DR OrthoDB; 447457at2759; -.
DR PRO; PR:F4KCL7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KCL7; baseline and differential.
DR Genevisible; F4KCL7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IMP:CACAO.
DR GO; GO:0006626; P:protein targeting to mitochondrion; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 3.90.1300.10; -; 1.
DR InterPro; IPR023631; Amidase_dom.
DR InterPro; IPR036928; AS_sf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF01425; Amidase; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR SUPFAM; SSF75304; SSF75304; 1.
DR PROSITE; PS50005; TPR; 3.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Protein transport; Reference proteome;
KW Repeat; TPR repeat; Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..603
FT /note="Outer envelope protein 64, mitochondrial"
FT /id="PRO_0000414024"
FT TRANSMEM 16..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 488..521
FT /note="TPR 1"
FT REPEAT 523..555
FT /note="TPR 2"
FT REPEAT 556..589
FT /note="TPR 3"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT HELIX 484..500
FT /evidence="ECO:0007829|PDB:6HPG"
FT HELIX 504..517
FT /evidence="ECO:0007829|PDB:6HPG"
FT HELIX 522..534
FT /evidence="ECO:0007829|PDB:6HPG"
FT HELIX 538..551
FT /evidence="ECO:0007829|PDB:6HPG"
FT HELIX 556..568
FT /evidence="ECO:0007829|PDB:6HPG"
FT HELIX 572..585
FT /evidence="ECO:0007829|PDB:6HPG"
FT HELIX 590..600
FT /evidence="ECO:0007829|PDB:6HPG"
SQ SEQUENCE 603 AA; 65912 MW; 6D4191B24EB9205F CRC64;
MSNTLSLIQS NASNPKVWVV IGVTVAGIVI LAETRKRRIR ALREEDFGAF LDRFELLPFP
PPPPPAAKQS LSGLTFSISD AFDVKDYITG FGCPQWKKTH EAAEKTAVVV TTLLKNGATC
VGKTIMDELG FGIIGENKHY GTPINPLMPD NVPGGCSSGS AVSVGAELVD FSLGIDTTGG
VRVPAAFCGI LGFRPSQGTV SSVGVLPNSQ SLETVGWFAS DPSVLCQVGH ALLNLSAVTH
RRQRSLIFAD DLFELSDIPK QKSVQVVRKA IENLSGYKTP KHVNVGQYVA SNVPSLAEFC
EQSGKSQNSA STLRALSSVM LAIQRHEFKT NHEEWWQTCK SFLGPRFSND VVTALKSKNE
SIKSLYRVKN EMRATIQSLL KEDGILVIPT VADPPPRLNT KRNKSLNEFL DRTYALSCIA
SMSGCCQVTI PLGEHGDRPI SVSLLTYYGG DKFLLDTTLD VYASLQDQAK LASNLAPVSD
TNGNMEASEV MKEKGNAAYK GKQWNKAVNF YTEAIKLNGA NATYYCNRAA AFLELCCFQQ
AEQDCTKAML IDKKNVKAYL RRGTARESLV RYKEAAADFR HALVLEPQNK TAKVAEKRLR
KHI
